Dataset: DOC uptake rates by coccolithophores and scintillation counts from field experiments in the North Atlantic during R/V Endeavor cruise EN616 in July 2018

ValidatedFinal no updates expectedDOI: 10.26008/1912/bco-dmo.887562.1Version 1 (2023-03-22)Dataset Type:Cruise ResultsDataset Type:experimental

Principal Investigator: William M. Balch (Bigelow Laboratory for Ocean Sciences)

Co-Principal Investigator: Stephen D. Archer (Bigelow Laboratory for Ocean Sciences)

Co-Principal Investigator: David T. Drapeau (Bigelow Laboratory for Ocean Sciences)

Co-Principal Investigator: Jelena Godrijan (Bigelow Laboratory for Ocean Sciences)

BCO-DMO Data Manager: Dana Stuart Gerlach (Woods Hole Oceanographic Institution)


Project: Coccolithophore Mixotrophy (Cocco-Mix)


Abstract

Field experiments were performed in the NW Atlantic during R/V Endeavor cruise EN616 in July 2018 to determine the potential uptake of dissolved organic carbon (DOC) by coccolithophores to compare with the bicarbonate uptake associated with standard photosynthesis and calcification. Waters from the continental shelf and slope, plus Sargasso Sea were sampled. Natural phytoplankton populations were incubated with radiolabeled dissolved organic carbon compounds (14C-acetate, 14C-mannitol, and 14C-...

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This dataset is part of a larger study with the following goals:

  •  Goal #1: measure the mixotrophic uptake and assimilation of 14C-acetate, 14C-mannitol and 14C-glycerol as a carbon source by natural assemblages of coccolithophores and compare it to their autotrophic uptake and assimilation of DIC. These three organics were chosen due to their high potential for significant osmotrophy by coccolithophores as seen in previous culture studies (Godrijan et al. (2020) and related dataset: https://www.bco-dmo.org/dataset/858771). The design of these experiments used radiochemical and single cell/flow cytometer methods to distinguish osmotrophy by coccolithophores from that of other naturally-occurring microalgae.
  • Goal #2: test for the fixation of 14C-labeled organics into both POC and PIC fractions in natural populations of coccolithophores, in order to examine the potential role of coccolithophore osmotrophy in the biological carbon pump and alkalinity carbon pump paradigms.

Related Datasets

IsRelatedTo

Dataset: Coccolithophore birefringence from polarized microscopy
Balch, W. M., Archer, S. D., Drapeau, D. T., Godrijan, J. (2023) Coccolithophore counts from polarized microscopy birefringence measurements of samples collected in the Northwest Atlantic during R/V Endeavor cruise EN616 in July 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-02-05 doi:10.26008/1912/bco-dmo.887863.1
IsRelatedTo

Dataset: FlowCAM imaging cytometer data from EN616 cruise
Balch, W. M., Archer, S. D., Drapeau, D. T., Godrijan, J. (2023) FlowCAM enumeration of phytoplankton classes from samples taken during R/V Endeavor cruise EN616 in July 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-01-27 doi:10.26008/1912/bco-dmo.887787.1
IsSupplementedBy

Dataset: Data for ambient concentrations of three DOC compounds (acetate, glycerol, mannitol)
Balch, W. M., Archer, S. D., Drapeau, D. T., Godrijan, J. (2023) Ambient concentrations of acetate, glycerol, and mannitol measured from samples collected during R/V Endeavor cruise EN616 in the northwest Atlantic in July 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-02-26 doi:10.26008/1912/bco-dmo.887851.1
IsSupplementedBy

Dataset: EN616 CTD hydrography
Balch, W. M., Archer, S. D., Drapeau, D. T., Godrijan, J. (2023) Hydrography and environmental conditions measured with CTD at nine stations during R/V Endeavor cruise EN616 in July 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-02-24 doi:10.26008/1912/bco-dmo.887800.1

Related Publications

Results

Balch, W. M., Drapeau, D. T., Poulton, N., Archer, S. D., Cartisano, C., Burnell, C., & Godrijan, J. (2023). Osmotrophy of dissolved organic compounds by coccolithophore populations: Fixation into particulate organic and inorganic carbon. Science Advances, 9(21). https://doi.org/10.1126/sciadv.adf6973
Methods

Balch, W. M., Drapeau, D. T., & Fritz, J. J. (2000). Monsoonal forcing of calcification in the Arabian Sea. Deep Sea Research Part II: Topical Studies in Oceanography, 47(7–8), 1301–1337. https://doi.org/10.1016/s0967-0645(99)00145-9
Methods

Paasche, E., & Brubak, S. (1994). Enhanced calcification in the coccolithophorid Emiliania huxleyi (Haptophyceae) under phosphorus limitation. Phycologia, 33(5), 324–330. https://doi.org/10.2216/i0031-8884-33-5-324.1
Related Research

Godrijan, J., Drapeau, D., & Balch, W. M. (2020). Mixotrophic uptake of organic compounds by coccolithophores. Limnology and Oceanography, 65(6), 1410–1421. doi:10.1002/lno.11396
Related Research

Hewes, C. D., & Holm-Hansen, O. (1983). A method for recovering nanoplankton from filters for identification with the microscope: The filter-transfer-freeze (FTF) technique. Limnology and Oceanography, 28(2), 389–394. Portico. https://doi.org/10.4319/lo.1983.28.2.0389