Amplicon sequences related to this dataset are available via NCBI BioProject PRJNA672129, and metagenome and metatranscriptome sequences are available via BioProject PRJNA779602. MAGs are associated with the same BioProject and are individually accessible via BioSamples SAMN23474158 - SAMN23474187. In addition, GenBank accessions are listed for each MAG in Table S4. Protocols, metadata, and additional data are provided in a Zenodo-archived GitHub repository, Brazelton-Lab/Brazelton-2022-Lost-City-fluids-MAGs: Final, published versions of text included. Citations and links to these resources can be found within the Related Datasets section of this metadata page.