Dataset: Nematostella ocean acidification experiment: Larval heat tolerance

Name: Larval heat tolerance of Nematostella vectensis following parental exposure to ocean acidification during lab experiments conducted in spring 2022.
Published: 2024-03-24
Keywords: oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.920813.1

Laboratory at the University of Pennsylvania

Temporal Extent: 2022-01-26 - 2022-05-02

Project:

Influence of environmental pH variability and thermal sensitivity on the resilience of reef-building corals to acidification stress (Coral Resilience)

Principal Investigator:

Katie Barott (University of Pennsylvania, Penn)

Scientist:

Kristen Brown (University of Pennsylvania, Penn)

Kelsey Speer (University of Pennsylvania, Penn)

Student:

Benjamin Glass (University of Pennsylvania, Penn)

Angela Schmitt (University of Pennsylvania, Penn)

Contact:

Benjamin Glass (University of Pennsylvania, Penn)

BCO-DMO Data Manager:

Karen Soenen (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2024-03-24

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Larval heat tolerance of Nematostella vectensis following parental exposure to ocean acidification during lab experiments conducted in spring 2022.

Abstract:

Ocean acidification (OA) resulting from anthropogenic CO2 emissions is impairing the reproduction of marine organisms. While parental exposure to OA can protect offspring via carryover effects, this phenomenon is poorly understood in many marine invertebrate taxa. We examined how parental exposure to acidified (pH 7.40) versus ambient (pH 7.72) seawater influenced reproduction and offspring performance across six gametogenic cycles (13 weeks) in the estuarine sea anemone Nematostella vectensis. This dataset pertains to the performance of larvae following parental exposure to ocean acidification, specifically larval heat tolerance.

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Archival Copy

Version Date	Archive	Persistent Identifier	Date Assigned
2024-03-24	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.920813.1	2024-03-24

Description

Data generated as part of a Nematostella ocean acidfication experiment published in Glass et al., 2023. (see Related Publications). Related Zenodo datasets provides further analysis and plotting of the BCO-DMO dataset here. (see Related Dataset).

Methods & Sampling

Nematostella vectensis (Stephenson, 1935) anemones were collected from a salt marsh in Brigantine, New Jersey in the fall of 2020. Females were identified by inducing spawning, and 14 individuals that released eggs were chosen as the genotype pool for this experiment. Each female was then horizontally bisected through the body column using a razor blade, resulting in two genotypically identical individuals that were divided between the two experimental groups (ambient and acidic).

A clonal male population, also originating from the United States Atlantic coast, was obtained from the laboratory of Dr. Katerina Ragkousi (Amherst College) in the spring of 2021. The male population size was increased via bisection, resulting in a total of 20 genetically identical males for the experiment (N=10 per treatment).

All anemones were kept in 12 parts per thousand (ppt) artificial seawater (ASW; Instant Ocean Reef Crystals^Ⓡ reef salt, Spectrum Brands, Blacksburg, VA, USA) at pH 7.7–8.1 and 18°C. The animals were maintained in a dark incubator (Boekel Scientific, Feasterville-Trevose, PA, USA) and fed approximately every other day with Artemia nauplii. The experiment was performed approximately 1–1.5 years after animal collection.

Data Processing Description

Larvae from each parental treatment were individually pipetted into polymerase chain reaction (PCR) strip tubes (N=32 larvae treatment⁻¹ temperature⁻¹). Larvae were then exposed to one of a range of peak temperatures between 39–43°C in 0.5-degree increments. MiniAmp thermal cyclers (Thermo Fisher Scientific, Waltham, MA, USA) were used for heat ramps, which were programmed as follows: (1) 1 min at 25°C; (2) 4 min at 30°C; (3) 4 min at 38°C; (4) 1 h at the peak temperature (39–43°C); (5) 4 min at 38°C; (6) 4 min at 30°C; (7) infinite hold at 22°C.

Strip tubes containing larvae were capped, randomly assigned to positions in the thermal cyclers for the heat ramp, and then removed and uncapped as soon as the cool-down ramp was complete. Following uncapping, tubes were placed in the dark at 18°C for 48 h, then larvae were transferred with a multichannel pipette to a 96-well plate and examined for survival (no clear disintegration of tissue) under a dissecting microscope. The percentage of larvae surviving after exposure to each peak temperature was calculated as the number of larvae surviving divided by the total number of larvae exposed to each temperature.

For heat tolerance data, the percentage of larvae surviving after exposure to each peak temperature was calculated as the number of larvae surviving divided by the total number of larvae exposed to each temperature

BCO-DMO Processing

* Adjusted parameter names to comply with database requirements

Data Files

File
920813_v1_larvalheattolerance.csv (Comma Separated Values (.csv), 334 bytes) MD5:ba6e9a36f6142ce8bed1ecf18324012b Primary data file for dataset ID 920813, version 1

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1923743

Deployments

None available yet

Instruments

Thermal Cycler

Supplied Name: MiniAmp thermal cyclers (Thermo Fisher Scientific, Waltham, MA, USA)

Supplied Description:

MiniAmp thermal cyclers (Thermo Fisher Scientific, Waltham, MA, USA) for thermal tolerance heat ramps

Instrument Type

Generic Name: Thermal Cycler

Generic Description:

A thermal cycler or "thermocycler" is a general term for a type of laboratory apparatus, commonly used for performing polymerase chain reaction (PCR), that is capable of repeatedly altering and maintaining specific temperatures for defined periods of time. The device has a thermal block with holes where tubes with the PCR reaction mixtures can be inserted. The cycler then raises and lowers the temperature of the block in discrete, pre-programmed steps. They can also be used to facilitate other temperature-sensitive reactions, including restriction enzyme digestion or rapid diagnostics.

(adapted from http://serc.carleton.edu/microbelife/research_methods/genomics/pcr.html)

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Temperature	Temperature at which larvae were treated for 1-hour as a heat shock	degrees Celsius (°C)	temperature
Treatment	Experimental treatment into which anemones were placed (ambient or acidic seawater pH)	unitless	treatment
Percent_surviving_larvae	The percent of larvae surivivng 48 hours after the heat shock	percentage (%)	relative_abund

Database

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Dataset: Nematostella ocean acidification experiment: Larval heat tolerance