Dataset: Specific Dynamic Action of larval grunion - Experiments 2a and 2b

Name: Differences in mean oxygen consumption of fed and unfed larvae used to understand the metabolic cost of digestion, Specific Dynamic Action (SDA), under ocean acidification and warming treatments - Experiments 2a and 2b
Published: 2024-04-09
Keywords: Specific Dynamic Action, ocean acidification, ocean warming, oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.924613.1

nearshore waters of Southern California

Temporal Extent: 2021-08-09 - 2022-04-27

Project:

RUI: Evaluating selection via ocean acidification and evolutionary responses of two coastal fishes (OA and natural selection)

Principal Investigator:

Darren Johnson (California State University Long Beach, CSULB)

BCO-DMO Data Manager:

Shannon Rauch (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2024-04-09

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Differences in mean oxygen consumption of fed and unfed larvae used to understand the metabolic cost of digestion, Specific Dynamic Action (SDA), under ocean acidification and warming treatments - Experiments 2a and 2b

Abstract:

These data include the differences in mean oxygen consumption of fed and unfed larvae used to understand the metabolic cost of digestion, Specific Dynamic Action (SDA), under ocean acidification and warming treatments. Data was collected in the summers of 2021 and 2022 using a microplate reader system that uses optical fluorescence to measure dissolved oxygen concentrations in water. Knowing the energetic cost of digestion under future climate change is important as studies, particularly on larval fish, begin to investigate how energy budgets will change. These data help us to understand an important part of daily metabolic costs and how that cost might change under ocean acidification and warming. Data were collected by Emma Siegfried and Dr. Darren Johnson at California State University, Long Beach. These data are from experiments 2a and 2b. Experiment 2a contains delta VO2 values to describe the SDA curve for a single feeding under 4 experimental treatments (low temperature & low CO2; low temperature & high CO2; high temperature & low CO2; high temperature & high CO2). In experiment 2a, food ration was increased at high temperatures. Experiment 2b contains delta VO2 values to describe the SDA curve for a single feeding under the same 4 experimental treatments, except that food ration was held constant across all temperature and CO2 treatments.

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Archival Copy

Version Date	Archive	Persistent Identifier	Date Assigned
2024-04-09	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.924613.1	2024-04-09

Methods & Sampling

To measure oxygen consumption, individual larvae were placed within chambers of a closed microplate reader system that uses optical fluorescence to measure oxygen concentration (PreSens, Germany). After a 5-minute acclimation period, the change in dissolved oxygen was measured every 30 seconds over 20 minutess. The microplate reader had 2 blocks of 24 wells.

In Experiment 1 (see Related Datasets), 20 fish larvae were measured per run. In Experiment 2, 40 fish were measured per run. For all chambers, the investigators ran a linear regression of oxygen concentration on time (n = 40 measurements). The respiration rate for each fish (VO2, expressed in milligrams of O2 per individual per hour (mg O2 ind-1 h-1)) was calculated as:

VO2 = V (S – B)

where S is the slope describing the change in O2 concentration for individual chambers with the fish,
B is the average slope for the four chambers with no fish (both in units of milligrams O2 per liter per hour (mg O2 L-1 h-1) and inferred to be per individual since each well held only one larvae), and
V is the volume of water in the chamber (1.500 × 10-3 liters (L)).

Note the displacement volume of grunion larvae in this study was <2.5 × 10-6 L and thus negligible in these calculations. Fish were assigned to each chamber at random, and larvae were used once in the respiration measurements and then humanely euthanized.

To describe the duration and magnitude of the Specific Dynamic Action (SDA) response, the investigators first calculated delta.VO2, the difference between the mean rates of oxygen consumption for the pair of fed and non-fed larvae.

These data were summarized by Siegfried and Johnson (2023) and plotted in figures 1, 3, and 5.

Data Processing Description

For all chambers, the investigators ran a linear regression of oxygen concentration on time to obtain the respiration rate of each fish. Respiration rates, referred to as VO2, were expressed in mg O2 ind-1 h-1. Average VO2 values were then calculated for groups of fed and non-fed larvae of the same age and average size, and experiencing the same seawater conditions. The difference in average VO2 values (referred to as delta VO2) provides a measure of the average energetic cost of digestion at a single time post feeding.

BCO-DMO Processing

- Imported original files "Expt 2a summary file.xlsx" and "Expt 2b summary file.xlsx" into the BCO-DMO system.
- Concatenated the data from both files into a single dataset.
- Renamed fields to comply with BCO-DMO naming conventions.
- Marked "#VALUE!" as a missing data value (missing data are blank/empty in the final CSV file).
- Saved the final file as "924613_v1_sda_expts2.csv".

Data Files

File
924613_v1_sda_expts2.csv (Comma Separated Values (.csv), 15.33 KB) MD5:6bf9b19ee4070b5fe669ea9c06e4e5cb Primary data file for dataset ID 924613, version 1

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1948975

Deployments

None available yet

Instruments

plate reader

Supplied Name: closed microplate reader system

Supplied Description:

Oxygen consumption was measured within a closed microplate reader system that uses optical fluorescence to measure oxygen concentration (Loligo Systems, Denmark and PreSens, Germany).

Instrument Type

Generic Name: plate reader

Generic Description:

Plate readers (also known as microplate readers) are laboratory instruments designed to detect biological, chemical or physical events of samples in microtiter plates. They are widely used in research, drug discovery, bioassay validation, quality control and manufacturing processes in the pharmaceutical and biotechnological industry and academic organizations. Sample reactions can be assayed in 6-1536 well format microtiter plates. The most common microplate format used in academic research laboratories or clinical diagnostic laboratories is 96-well (8 by 12 matrix) with a typical reaction volume between 100 and 200 uL per well. Higher density microplates (384- or 1536-well microplates) are typically used for screening applications, when throughput (number of samples per day processed) and assay cost per sample become critical parameters, with a typical assay volume between 5 and 50 µL per well. Common detection modes for microplate assays are absorbance, fluorescence intensity, luminescence, time-resolved fluorescence, and fluorescence polarization. From: http://en.wikipedia.org/wiki/Plate_reader, 2014-09-0-23.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
experiment_ID	Experiment ID; either 2a or 2b	unitless	exp_id
DPH	Age in days post hatching	days	age
deltaVO2	difference in average oxygen consumption rates between fed and unfed larvae	milligrams O2 per liter per hour	O2_consum
deltaVO2_SE	Standard error of the difference in average oxygen consumption rates between fed and unfed larvae	milligrams O2 per liter per hour	O2_consum
temperature	Temperature	degrees Celsius	temp
OA_treat	short description of the combinations of temperature and pCO2	unitless	treatment
pCO2	Partial pressure of carbon dioxide in seawater	microatmospheres	pCO2
time_elapsed	Time after feeding, in hours	hours	time_elapsed

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Dataset: Specific Dynamic Action of larval grunion - Experiments 2a and 2b