Dataset: Data and code from an examination of growth rates of cyanobacteria co-cultured with a heterotrophic bacterium, Alteromonas, under either present-day or predicted future pCO2 conditions

Contains growth rates for experiments done at the end of the experiment comparing the ancestral and evolve phytoplankton organisms. Each row represents a single transfer cycle as in the above description. Column headings are: Strain: which phytoplankton strain was measured -- Prochlorococcus MIT9312, Synechococcus CC9311, Thalassiosira oceanica CCMP1005, or Emiliania huxleyi CCMP371. Evol...

Contains data on representative growth curves of the four main phytoplankton used in the evolution experiment. This data was used to produce Figure S22A-D

Contains the day-by-day flow cytometry data collected during the evolution phase of the Long Term Phytoplankton Evolution experiment that forms the basis of the manuscript. Each row represents the results of a single transfer cycle of a single evolving lineage; i.e., the beginning and ending cell densities, dates, and so forth. These data were used to generate main text Figure 1 Lu, et al. (2025)....

Contains chemical data about the medium batches used for the evolution experiment. This data was used to produce Figure S20.

Contains the data from experiments where ancestral and evolved Prochlorococcus were grown with different strains of Alteromonas EZ55 (or axenically). These data were used to generate main text Figure 4B of Lu, et al. (2025). Column headings are as follows: LTPE: LTPE strain designation of the Prochlorococcus strain in the experiment. Pro: Whether the Prochlorococcus strain was ancestral (...

Contains the data from experiments measuring the impact of different EZ55 strains or axenic culture on mortality of Prochlorococcus strains. These data were used to make Figure 4A Lu, et al. (2025). Column headings are as follows: LTPE: LTPE Strain designation of the Prochlorococcus strain in the experiment. Pro: Whether the Prochlorococcus strain was ancestral (Anc), evolved at 400 ppm p...

Contains data on the change in pH over the time between culture inoculation and culture transfer during the evolution experiment (see Methods, Lu, et al. 2025). This data was used to produce Figure S22E.

Contains data where the pH was re-measured after some number of days, as described in the Methods (Lu et al 2025). This data was used to produce Figure S21.

Contains the code necessary to run the statistical analyses described in the text (Lu, et al., 2025).

Explanation of the files in this dataset, how the data files are organized, and how to run the code to replicate the analyses we used in our manuscript (Lu, et al., 2025).