©2026 Biological and Chemical Oceanography Data Management Office.Sponge exhalent water was collected from Looe Key reef, coral exudates water was collected from a coral incubation at Mote Marine lab, and ambient reef water was collected from Looe Key Reef surface water. All seawater was 0.2 um filtered to use as media in 2L bottles for an incubation experiment. Inoculum from reef surface water (1.6 um filtered) was used for the incubation. Bottles were incubated in the dark for 48 hours and samples were taken at the start (T0) and end (T48) for nutrient and microbiome analysis.
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Seawater sampled from the incubation ‘soup’ or bottles was used for multiple nutrient analyses and DNA extraction.
The rest of the seawater was filtered through an Omnipore 0.2 um filter using a peristaltic pump and acid and milliQ water rinsed Pharmed BP tubing and Teflon filter holders. The filter was stored at -80C until DNA extraction.
The NCBI dataset is the microbiome profiling data of the incubation experiment. These are the reads of 16S rRNA genes for each sample in the incubation experiment. Bottle incubations were set up as described in the methods for dataset 963872. The seawater from the bottles were filtered at T0 and at T48 and the filters were used for DNA extraction, amplification of the v4 region of the 16S rRNA gene, and Illumina sequencing. Additional details available through the NCBI accession link.
Incubation experiment.
Fiore, C. L., Easson, C. G., Apprill, A., Reigel, A. M., Fortier, K., Moore, D. (2025). Microbiome profiling of bacterioplankton communities on sponge excurrent water, coral exudate water, and surface reef water.. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-06-17 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.964182.1 [access date]
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This dataset is licensed under Creative Commons Attribution 4.0.
If you wish to use this dataset, it is highly recommended that you contact the original principal investigators (PI). Should the relevant PI be unavailable, please contact BCO-DMO (info@bco-dmo.org) for additional guidance. For general guidance please see the BCO-DMO Terms of Use document.