Water samples were collected for DNA extraction using sampling bottles deployed on the CTD rosette. At each depth, 2-liter (L) samples were filtered through 0.22-micrometer (µm) filters (Millipore, Darmstadt, Germany) using a peristaltic pump and frozen until extraction. DNA was extracted following Beman et al. (2012) and sent for metagenome sequencing in the Vincent J. Coates Genome Sequencing Laboratory (GSL) at the University of California, Berkeley. For each sample, 250 ng of genomic DNA was sheared and libraries were prepared using the KAPA HyperPrep Kit (Kapa Biosystems, Wilmington, MA, USA). Samples were pooled into a single lane and sequenced via 150- cycle paired-end sequencing on the Illumina HiSeq 4000 platform (Illumina, Inc., San Diego, CA, USA).