©2026 Biological and Chemical Oceanography Data Management Office.Funded by the U.S. National Science Foundation
©2026 Biological and Chemical Oceanography Data Management Office.This dataset contains BioSample/SRA accession identifiers and links for metatranscriptomic samples from seawater homarine-addition incubation experiments conducted during R/V Rachel Carson cruise RC0078 in June 2022 in the Salish Sea near the Strait of Juan de Fuca. Treatments included homarine-amended and unamended control seawater used to identify microorganisms and genes responsive to homarine.
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CTD data were collected in the Salish Sea on the R/V Rachel Carson, cruise RC0078 in June 2022.
Seawater was transferred to acid-clean, blue-tinted, 5-L polycarbonate carboys for acclimation in a deck incubator with flow-through surface seawater. A set of three carboys were supplied with 0.1 mM carbon as homarine and three other unamended carboys served as control group. We incubated samples for 3.5 hours and proceeded to concentrate the microbial fraction by filtering each through a 47 mm-diameter, 0.22 µm pore-size polycarbonate membrane with a peristaltic pump. The filters were flash-frozen in liquid nitrogen at sea and were transferred to -80°C storage in the lab.
Sosa, O. A., Ingalls, A. E., Heal, K. (2026). Sequence data accession numbers originating from seawater homarine addition experiments conducted on R/V Rachel Carson RC0078 cruise in the Salish Sea in June 2022. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2026-04-22 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/997408 [access date]
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