Dataset: Sequence data accession numbers originating from seawater homarine addition experiments conducted on R/V Rachel Carson RC0078 cruise in the Salish Sea in June 2022

CTD data were collected in the Salish Sea on the R/V Rachel Carson, cruise RC0078 in June 2022.

Seawater was transferred to acid-clean, blue-tinted, 5-L polycarbonate carboys for acclimation in a deck incubator with flow-through surface seawater. A set of three carboys were supplied with 0.1 mM carbon as homarine and three other unamended carboys served as control group. We incubated samples for 3.5 hours and proceeded to concentrate the microbial fraction by filtering each through a 47 mm-diameter, 0.22 µm pore-size polycarbonate membrane with a peristaltic pump. The filters were flash-frozen in liquid nitrogen at sea and were transferred to -80°C storage in the lab.

The samples were sequenced at SeqCenter in Pittsburgh, PA. Samples were DNAse treated with Invitrogen DNAse (RNAse free). Library preparation was performed using Illumina’s Stranded Total RNA Prep Ligation with Ribo-Zero Plus kit and 10bp IDT for Illumina indices. Sequencing was done on a NovaSeq 6000 giving 2x51bp reads. Demultiplexing, quality control, and adapter trimming was performed with bcl-convert (v4.0.3, Illumina).