Methods on EN667:
One liter (L) samples were collected via CTD rosette for particulate lipid, carbohydrate, and organic carbon samples. Samples were filtered onto 47-millimeter (mm) 0.2-micrometer (um) Durapore filters (lipids, carbs; Millipore) and 25 mm 0.7 um GF/F filters (POC; Whatman), flash frozen in liquid nitrogen, and stored in LN2 headspace until extraction.
Lipid samples were processed and analyzed according to Holm et al. (2022), and quantified via Holm et al. (2024). Per-liter particulate lipid values are reported in peak area and femtomoles per liter. All values for all compounds were blank-corrected using the average of five 1-L filtered seawater blanks collected from the sea surface on EN667.
Methods on SR2310:
One liter samples were collected via CTD rosette for particulate lipid, carbohydrate, and organic carbon samples. Samples were filtered onto 47 mm 0.2 um Durapore filters (lipids, carbs; Millipore) and 25 mm 0.7 um GF/F filters (POC; Whatman), flash frozen in liquid nitrogen, and stored in LN2 headspace until extraction.
Lipid samples were processed and analyzed according to Holm et al. (2022), and quantified via Holm et al. (2024). Per-liter particulate lipid values are reported in peak area and femtomoles per liter. All values for all compounds were blank-corrected using the average of twenty 0.2 um Durapore filters processed and analyzed alongside sample filters.
Methods on AE2320:
Two liter samples were collected via CTD rosette for particulate lipid, carbohydrate, and organic carbon samples. Samples were filtered onto 47 mm 0.2 um Durapore filters (lipids, carbs; Millipore) and 25 mm 0.7 um GF/F filters (POC; Whatman), flash frozen in liquid nitrogen, and stored in LN2 headspace until extraction.
Lipid samples were processed and analyzed according to Holm et al. (2022), and quantified via Holm et al. (2024). Per-liter particulate lipid values are reported in peak area and femtomoles per liter. All values for all compounds were blank-corrected using the average of seven 2-L filtered seawater blanks collected from the surface on AE2320.