©2026 Biological and Chemical Oceanography Data Management Office.This dataset represents the shotgun metagenome samples used in Zhao et al., ISME J 2025. Samples for metagenomic sequencing were collected from the oxygen minimum zone in the Eastern Tropical Pacific Ocean during the R/V Sally Ride (SR2114) cruise, December 2021 – January 2022. Sea water was collected from all nine depths for all five sampling stations reported in the Zhao et al., paper (See Table for details). Collections were made using Niskin bottles on a rosette containing a conductivity–tem...
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Samples for metagenomic sequencing were collected from the Eastern Tropical North Pacific (ETNP) Oxygen Minimum Zone (OMZ) on R/V Sally Ride (SR2114) from December 2021 to January 2022. Sea water for metagenomes was collected from all nine depths for all five sampling stations. Collections were made using Niskin bottles on a rosette containing a conductivity–temperature–depth profiler (Sea-Bird SBE 911plus), as described in Tsementzi et al., 2016.
DNA was extracted from biomass on collecting filters using the MoBio Power Soil kit (MoBio Inc. Carlsbad, CA, USA) and libraries were prepared for metagenomic sequencing using the Illumina DNA library prep kit with unique dual indexing according to manufacturer's instructions, except that the protocol was terminated after isolation of cleaned double stranded libraries. An equimolar mixture of the libraries was sequenced on an Illumina NovaSeq 6000 instrument at the Molecular Evolution Core, Georgia Institute of Technology.
Konstantinidis, K. (2026). Sample information and accession numbers for metagenome samples collected in the Eastern Tropical Pacific Oxygen Minimum Zone on R/V Sally Ride cruise SR2114 during December 2021 to January 2022. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2026-05-18 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.998771.1 [access date]
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This dataset is licensed under Creative Commons Attribution 4.0.
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