©2026 Biological and Chemical Oceanography Data Management Office.This dataset describes assembled genomes of single-cell amplified genomes (SAGs) used in Zhao et al., ISME 2025. Water samples were collected during conductivity–temperature–depth (CTD) casts from an oxygen minimum zone in the Eastern Tropical Pacific Ocean, where the SAR11 make up ~20% of the total microbial community, during the R/V Atlantis cruise AT50-08. Sorting was performed on 4 April 2023 (within 2 months from the date the first sample was collected) and SAGs were generated with the modi...
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Water samples were collected using Niskin bottles on a rosette containing a conductivity–temperature–depth profiler (Sea-Bird SBE 911plus). The water was prepared by cryopreservation according to the protocol recommended by the Bigelow Single Cell Genomics Center (SCGC). Sorting was performed on 4 April 2023 (within <2 months from the date the first sample was collected) and SAGs were generated with the modified genomic DNA amplification technique, WGA-Y, which enables a substantially improved average genome recovery from single cells (service S-202). In total, 105 SAGs with Cp values <3 h were randomly selected for sequencing.
Genome assembly and draft annotation were performed by SCGC as described in the center’s webpage https://scgc.bigelow.org/capabilities/service-description/.
Konstantinidis, K. (2026). Single-cell amplified genomes (SAGs) collected during CTD casts in the Eastern Tropical Pacific Ocean on R/V Atlantis cruise AT50-08 during February-March 2023. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2026-06-10 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.998887.1 [access date]
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This dataset is licensed under Creative Commons Attribution 4.0.
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