©2026 Biological and Chemical Oceanography Data Management Office.Funded by the U.S. National Science Foundation
©2026 Biological and Chemical Oceanography Data Management Office.This dataset describes assembled genomes of single-cell amplified genomes (SAGs) used in Zhao et al., ISME 2025.The abstract of this manuscript follows: Surveys of microbial communities (metagenomics) or isolate genomes have revealed sequence-discrete species. That is, members of the same species show >95% average nucleotide identity (ANI) of shared genes among themselves vs.<83% ANI to members of other species while genome pairs showing between 83% and 95% ANI are comparatively rare. In these...
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Water samples were collected using Niskin bottles on a rosette containing a conductivity–temperature–depth profiler (Sea-Bird SBE 911plus). The water was prepared by cryopreservation according to the protocol recommended by the Bigelow Single Cell Genomics Center (SCGC). Sorting was performed on 4 April 2023 (within <2 months from the date the first sample was collected) and SAGs were generated with the modified genomic DNA amplification technique, WGA-Y, which enables a substantially improved average genome recovery from single cells (service S-202). In total, 105 SAGs with Cp values <3 h were randomly selected for sequencing.
Genome assembly and draft annotation were performed by SCGC as described in the center’s webpage https://scgc.bigelow.org/capabilities/service-description/.
Konstantinidis, K. (2026). SAGs from the Pacific Ocean OMZ. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2026-05-19 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/998887 [access date]
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This dataset is licensed under Creative Commons Attribution 4.0.
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