©2026 Biological and Chemical Oceanography Data Management Office.Funded by the U.S. National Science Foundation
©2026 Biological and Chemical Oceanography Data Management Office.This dataset corresponds to the raw sequences of the single-cell amplified genomes (SAGs) collected for the MicroPro project in the Eastern Tropical North Pacific oxygen minimum zone in February and March of 2023 aboard R/V Atlantis on cruise AT50-08B. It includes genome identifiers and associated metadata (e.g., sample IDs, collection context) and links each genome to its corresponding accession in the National Center for Biotechnology Information's Short Read Archive (NCBI SRA).
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Water samples (see data file for sampling depths and coordinates) were prepared by cryopreservation according to the protocol recommended by the Bigelow Single Cell Genomics Center (https://scgc.bigelow.org/wp-content/uploads/2018/06/Sample_cryopreservation_glyTE.pdf).
Briefly, small volumes of whole water (1 milliliter (ml)) were aliquoted under Helium (He) atmosphere using a glove box. Redox-Sensor Green (1 microliter (uL)) was added to the aliquots and left to incubate in the dark at in situ temperatures for 30 minutes. At the end of the incubation, glyTE (100 uL) was added and they were subsequently frozen at -80 degrees Celsius.
Sorting was performed on 4 April 2023 (within <2 months from the date the first sample was collected) and SAGs were generated with the modified genomic DNA amplification technique, WGA-Y, which enables a substantially improved average genome recovery from single cells (service S-202).
Pachiadaki, M. G. (2026). Genomic data collected from R/V Atlantis cruise AT50-08 in the Eastern Tropical North Pacific during February-March 2023. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2026-05-28 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/999321 [access date]
Terms of Use
This dataset is licensed under Creative Commons Attribution 4.0.
If you wish to use this dataset, it is highly recommended that you contact the original principal investigators (PI). Should the relevant PI be unavailable, please contact BCO-DMO (info@bco-dmo.org) for additional guidance. For general guidance please see the BCO-DMO Terms of Use document.