Dataset: Genomic data collected from R/V Atlantis cruise AT50-08 in the Eastern Tropical North Pacific during February-March 2023

Water samples (see data file for sampling depths and coordinates) were prepared by cryopreservation according to the protocol recommended by the Bigelow Single Cell Genomics Center (https://scgc.bigelow.org/wp-content/uploads/2018/06/Sample_cryopreservation_glyTE.pdf).

Briefly, small volumes of whole water (1 milliliter (ml)) were aliquoted under Helium (He) atmosphere using a glove box. Redox-Sensor Green (1 microliter (uL)) was added to the aliquots and left to incubate in the dark at in situ temperatures for 30 minutes. At the end of the incubation, glyTE (100 uL) was added and they were subsequently frozen at -80 degrees Celsius.

Sorting was performed on 4 April 2023 (within <2 months from the date the first sample was collected) and SAGs were generated with the modified genomic DNA amplification technique, WGA-Y, which enables a substantially improved average genome recovery from single cells (service S-202).