This dataset relates to both the growth (relative fluorescence) and growth rates datasets as these were the strains and conditions used to generate the growth data. Supplemental file 998934_v1_G_huxleyi_strain_growth_conditions.csv identifies the strain ID, the media type the strain was grown in, and the incubator temperature. The primary data file, 998934_v1_G_huxleyi_relative_fluorescence_units.csv, identifies the daily relative fluorescence units (in vivo chlorophyll a) as a proxy for biomass for the individual strains. Finally, the growth rates data file, 998934_v1_G_huxleyi_strain_exponential_daily_growth_rates.csv, identifies the exponential growth rate for all the strains over the linear portion of the growth curve.