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Instrument: Electron Microscope

Acronym:

External Identifier: skos:broadMatch: http://vocab.nerc.ac.uk/collection/L05/current/LAB07/

Description

Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of electrons behaving as waves.

Datasets (16)

Dataset Name	PI-Supplied Description	PI-Supplied Name
Alteromonas macleodii vesicle diameter measured during Cryo-EM micrograph experiment	The grids were observed on an FEI Tecnai F20 electron microscope (Thermo Fisher Scientific) operated at 200 kV with a typical magnification of ~34,500 × and 1-3 μm defocus.	FEI Tecnai F20 electron microscope
Microbial cell abundance and carbon, nitrogen, and sulfur content from venting fluids and microbial mats at Axial Seamount; from R/V Atlantis cruise AT18-08 in the Axial Seamount, Juan de Fuca Ridge in 2011	Subsamples of flocculent material were viewed under phase contrast, fixed and stained with DAPI for cell counting via epiflourescent microscopy, or fixed for scanning electron microscopy (SEM) with elemental detection system (EDS) on a Zeiss Supra 40VP.	Zeiss Supra 40VP
Carbonate data in experimental treatments of Emiliania huxleyi, 2011-2012 (E Hux Response to pCO2 project)	FEI Quanta 450 Scanning Electron Microscope	SEM
SEM based morphology of Emiliania huxleyi, 2011-2012 (E Hux Response to pCO2 project)	FEI Quanta 450 Scanning Electron Microscope	SEM
Estimated frequency of lytic viral infection from samples collected in the Eastern Tropical North Pacific oxygen minimum zone region (ETNP OMZ) on R/V New Horizon cruise NH1315 during June 2013		Philips CM12 transmission electron microscope (TEM)
Morphotypes, capsid widths, and tail lengths of viruses from samples collected in the Eastern Tropical North Pacific oxygen minimum zone region (ETNP OMZ) on R/V New Horizon cruise NH1315 during June 2013	Epifluorescence microscope (Axio Imager. D2, Zeiss, Jena, Germany)	Phillips CM12 transmission electron microscope
Results from shipboard high-pressure incubations of diffuse flow vent fluids collected from the Crab Spa and Alvinella sites at East Pacific Rise during the AT26-10 expedition, Jan. 2014 (Microbial Communities at Deep-Sea Vents project)	JSM-6500F field emission scanning electron microscope (JEOL)
SEM micrographs of selected common Mud Patch benthic forams from R/V Endeavor EN524 in the continental shelf off New England; 40.43 N 70.5 W from May 2013 (OA, Hypoxia and Warming project)		Scanning Electron Microscope
Light-dark calcification rates of Pleurochrysis carterae analyzed at Bigelow Laboratory in 2013 (OA Copes Coccoliths project)	Carl Zeiss Microscopy, LLC, Thornwood, NY, USA	Zeiss Supra25 field emission SEM
Pteropod shell dissolution in natural and high-CO2 environments from samples collected on RRS James Clark Ross cruise JR177 in the Scotia Sea, Southern Ocean from 2007-2008	SEM was done using a JEOL JSM 5900LV fitted with a tungsten filament at an acceleration voltage of 15 kV and a working distance of about 10 mm.	Scanning Electron Microscope
Experimental rearing of longfin squid under ocean acidification conditions from June 2011 (OA Squid Rearing project)	Zeiss NTS Supra 40VP with a field emissions source for the electrons and a JEOL JSM 35CF.	Microscope-Electron
Size and porosity of squid statolith from laboratory study from June to August 2011 (OA Squid Rearing project)	Zeiss NTS Supra 40VP with a field emissions source for the electrons and a JEOL JSM 35C	Microscope-Electron
Sulfate reduction rates at Main Endeavor grotto chimney from samples collected on RV Atlantis (AT18-08) during Jason II dives in the Juan de Fuca Ridge from July to August 2011	Tubeworm and macrofauna subsamples were imaged.	Zeiss model EVO Scanning Electron Microscope
Transmission electron micrographs of Pleurochrysis carterae CCMP645 cell thin sections from laboratory experiments at Bigelow Laboratory for Ocean Sciences, Maine from 2015-2016	Phillips/FEI EM201 transmission electron microscope operated at 100 kV.	Transmission Electron Microscope (TEM)
Transmission electron micrographs of virions from Pleurochrysis carterae CCMP645 culture supernatant from laboratory experiments at the Bigelow Laboratory for Ocean Sciences, Maine from 2015-2016	FEI Technai TEM at magnifications 6,000x – 250,000x at 100 kV	Transmission Electron Microscope (TEM)
Infectivity assays and ultrastructural characterization of Thalassia testudinum agroinfected with Turtle grass virus X (TGVX) in laboratory aquaria, February-May 2024	Transmission Electron Microscopy (TEM): JEOL JEM-1400 – used for imaging of virus-like particles and cytopathology at 80 kV.	JEOL JEM-1400