©2020 Biological and Chemical Oceanography Data Management Office.Funded by the U.S. National Science Foundation
©2020 Biological and Chemical Oceanography Data Management Office.A fully automated Segmented Flow Analysis (SFA) system, ideal for water and seawater analysis. It comprises a modular system which integrates an autosampler, peristaltic pump, chemistry manifold and detector. The sample and reagents are pumped continuously through the chemistry manifold, and air bubbles are introduced at regular intervals forming reaction segments which are mixed using glass coils. The AA3 uses segmented flow analysis principles to reduce inter-sample dispersion, and can analyse up to 100 samples per hour using stable LED light sources.
| Dataset Name | PI-Supplied Description | PI-Supplied Name |
|---|---|---|
| Alkalinity and dissolved inorganic carbon (DIC) from sea water samples collected at coral reefs from equatorial central Pacific in 1973, 2012, 2015, and 2018 | Some water samples were also analyzed to determine nitrate, ammonium, phosphate, and silicate concentrations at the Woods Hole Oceanographic Institution Nutrient Analytical Facility (https://web.whoi.edu/nutrient/) using a four channel segmented flow AA3 HR Autoanalyzer. | Four channel segmented flow AA3 HR Autoanalyzer |
| Seawater biogeochemistry inshore and offshore the Bermuda coral reef from the Bermuda coral reef platform and adjacent Sargasso Sea extending to Bermuda Atlantic Time Series-station from 2014-2018 | SEAL AutoAnalyzer III for inorganic nutrients. | SEAL AutoAnalyzer III |
| Bottle data from R/V Endeavor cruise EN665 in the Gulf of Maine, conducted April 7-12, 2021 | Nutrients were determined at the WHOI Nutrient Analytical Facility on a SEAL Analytical AA3 HR following established procedures. | SEAL Analytical AA3 HR |
| Measurements of C–P lyase phosphate thresholds in Stutzerimonas frequens cultures (North Pacific Methane Project) | Phosphorus concentrations were measured on 50 mL aliquots of culture medium using standard colorimetric methods with a SEAL Analytical AA3 autoanalyzer. | SEAL Analytical AA3 |
| Impacts of submarine groundwater discharge on benthic community composition and functional diversity on coral reefs in Mo'orea, French Polynesia from Aug 2021 to Jun 2022 | The samples were brought to the S-LAB at the University of Hawai‘i, where they were analyzed on a Seal Analytical AA3 HR Nutrient Analyzer (N+N: detection limit [DL] = 0.009 and coefficient of variation [CV] = 0.3%; PO43−: DL = 0.011 and CV = 0.2%; SiO32−: DL = 0.03 and CV = 0.5%). | Seal Analytical AA3 HR Nutrient Analyzer |
| Dissolved Nutrient Analyses for CTD water rosette samples and AUV Sentry/SUPR samples in the hydrothermal plumes emanating from the Main Endeavor region of the Juan de Fuca Ridge collected from R/V Atlantis cruise AT50-15 from Aug 28 to Sep 15 2023 | These samples were analyzed at the UC San Diego Oceanographic Data Facility using standard methods for dissolved nutrients (silicate, orthophosphate, nitrate, nitrite, and ammonium) in seawater. | |
| Carbonate chemistry analyses (total alkalinity, DIC, and d13C of DIC) from discrete bottle samples and CTD data from 12 stations sampled during R/V Endeavor cruise EN669 in the Gulf of Maine during August 2021 | Nutrients were determined at the WHOI Nutrient Analytical Facility on a SEAL Analytical AA3 HR following established procedures. | SEAL Analytical AA3 HR |
| Hydrography, nutrients, and nitrogen uptake rates from SPIROPA cruise TN368 in July 2019 | Samples to determine nitrate, phosphate, and silicate concentrations were filtered (0.4 μm, polycarbonate) directly from Niskin bottles into acid‐washed polyethylene bottles and stored frozen until analysis via standard colorimetric methods at the WHOI Nutrient Analytical Facility using a SEAL Analytical AA3 HR discrete chemistry analyzer. | SEAL Analytical AA3 HR discrete chemistry analyzer |
| Water nutrients measured at artificial reefs in Kāneʻohe Bay, Oʻahu in 2022 and 2023 as part of a reef halo dynamics study | Seal Analytical AA3 HR Nutrient Autoanalyzer: This instrument is a fully automated/computerized analyzing system for nutrients in environmental waters. It is a five-channel segmented-flow continuous analyzer consisting of a sampler, a pump, five-reagent mixing and reaction manifolds and five photometers. S-LAB utilizes the AA3 to simultaneously measure dissolved inorganic nitrate, nitrite, ammonium, phosphate, and silicate at low μM levels. Detection limits offered by this state-of-the-art instrument fulfill the detection needs of researchers wishing to analyze oligotrophic (low nutrient concentration) seawater, while also providing the capability of analyzing fresh and brackish waters, including soil water, sediment pore water, and groundwater. (Description from https://www.soest.hawaii.edu/S-LAB/equipment/slab_autoanalyzer.htm | Seal Analytical AA3 HR Nutrient Autoanalyzer |
| Nutrient and chlorophyll analyses from surface samples collected underway on board container ships during basin-wide transects of the North Pacific Ocean from Hong Kong to Long Beach, CA from 2009-2012 | Seal Analytical AA3 | |
| Pseudo-nitzschia spp. presence-absence and environmental data in Narragansett Bay in Rhode Island, USA and the Northeast U.S. Shelf (NES-LTER transect) from 2018-2023 | Manufactured by SEAL Analytical, Mequon, WI, USA | AA3 HR Autoanalyzer |
| Reef organism growth from in-situ experiments with submarine groundwater discharge (SGD) exposure treatments conducted in Mo'orea, French Polynesia in 2022 and 2023 | Seal Analytical AA3 HR Nutrient Analyzer at the University of Hawaii S-Lab : (N+N: detection limit [DL] = 0.009 and coefficient of variation [CV] = 0.3%; PO 3-: DL = 0.011 and CV = 0.2%; SiO 2-: DL = 0.03 and CV = 0.5%) was used to process all the nutrient samples | Seal Analytical AA3 HR |
| Carbon export from San Dieguito Lagoon from samples for seawater carbonate biogeochemistry between April 2014 and January 2015 | Nutrient samples were taken according to best practices and were filtered using a 0.4 µm filter and immediately frozen until analysis. Samples were analyzed using US Environmental Protection Agency methods for ammonium (method G-171-96, detection limit 0.034 µmol/L), nitrite+nitrate (method G-172-96, detection limit 0.010 µmol/L), silicate (method G-177-96, detection limit 0.016 µmol/L), and phosphate (method G-297-03, detection limit 0.025 µmol/L). Please see http://seal-analytical.com/Products/AA3SFAAnalyzer/tabid/59/Default.aspx for a complete instrument description. | SEAL AutoAnalyzer 3 four-channel segmented flow analyzer |
| CTD Bottle Data from R/V Sally Ride cruise SR2114 in the Eastern Tropical North Pacific, December 2021–January 2022 (N-loss in the ETNP ODZ project) | Nitrate (NO₃⁻), nitrite (NO₂⁻), phosphate (PO₄³⁻), and silicate (SiO₄⁴⁻) concentrations were measured at sea with a Seal AQ400 discrete autoanalyzer following standard chemical methods. | Seal AQ400 Discrete Autoanalyzer |
| TEP and CSP particle concentrations from samples collected from Station ALOHA to 31ºN in the North Pacific in June 2021 on the R/V Kilo Moana cruise KM2108 | Seal Analytical AA3 HR Nutrient Autoanalyzer | |
| Concentration measurements of water column phosphate, nitrate and nitrite, dissolved organic phosphorus, methane, and ethylene from samples collected during the R/V Neil Armstrong cruise AR16 in the western North Atlantic Ocean in May 2017 | SEAL Analytics autoanalyzer model 3 HR |