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Instrument: Ultra high-performance liquid chromatography

Acronym: UHPLC

External Identifier:

Description

Ultra high-performance liquid chromatography: Column chromatography where the mobile phase is a liquid, the stationary phase consists of very small (< 2 microm) particles and the inlet pressure is relatively high.

Datasets (18)

Dataset Name	PI-Supplied Description	PI-Supplied Name
Peptides associated with scaffold-derived metaproteomic proteins from samples taken during R/V Atlantic Explorer cruise AE1913 from the Sargasso Sea to Northeast US shelf waters in June of 2019		Dionex UltiMate 3000 RSLCnano LC system
Protein identification FASTA file (scaffold-derived metaproteomic proteins) from samples taken during R/V Atlantic Explorer cruise AE1913 from the Sargasso Sea to Northeast US shelf waters in June of 2019		Dionex UltiMate 3000 RSLCnano LC system
Scaffold-derived metaproteomic exclusive and total spectral counts associated with proteins from samples taken during R/V Atlantic Explorer cruise AE1913 from the Sargasso Sea to Northeast US shelf waters in June of 2019		Dionex UltiMate 3000 RSLCnano LC system
Benthic microalgal photopigment concentrations from nearshore shelf waters off Charleston, SC from June 2018 to August 2021	Shimadzu 10AD HPLC equipped with a monomeric (Rainin Microsorb-MV, 0.46 x 10 cm, 3 µm) and a polymeric (Vydac 201TP54, 0.46 x 25 cm, 5 µm) reverse-phase C18 column in series.	Shimadzu 10AD HPLC
Biochemical data on dissolved organic matter composition from Galveston Bay collected on five one-day trips aboard R/V Trident following Hurricane Harvey from September 4 to 28, 2017		Agilent Infinity 1260 series UHPLC system
Dissolved B vitamin and vitamer concentrations in surface seawater collected during the Malaspina Circumnavigation Expedition from December 2010 to July 2011	Thermo TSQ Altis Plus triple quadrupole mass spectrometer, coupled to a Vanquish Flex UHPLC system	Vanquish Flex UHPLC system
Sampling and accession information for extracellular reef seawater metabolites collected from the Jardines de la Reina reef-system, Cuba in November of 2017	Mass spectrometry samples were analyzed on a Vanquish UHPLC system (Thermo Fisher Scientific) coupled via heated electrospray ionization (H-ESI) to an ultrahigh resolution tribrid mass spectrometer, the Orbitrap Fusion Lumos (Thermo Fisher Scientific).	Vanquish UHPLC system (Thermo Fisher Scientific)
Pseudo-nitzschia spp. presence-absence and environmental data in Narragansett Bay in Rhode Island, USA and the Northeast U.S. Shelf (NES-LTER transect) from 2018-2023	Manufactured by Agilent Technologies, Santa Clara, CA, USA	1290 Infinity II UHPLC system
Biogeochemistry, metabolomics, and metagenomics of Florida's Coral Reef from sampling conducted over 15 days in June 2019	Ultrahigh performance liquid chromatography system (Vanquish UHPLC, Thermo ScientificTM) coupled with an Orbitrap Fusion Lumos Tribid mass spectrometer (Thermo ScientificTM) for untargeted metabolomics	Vanquish UHPLC, Thermo ScientificTM
Oceanographic data from 12 monthly cruises off the Israeli coast in the east Mediterranean conducted between Feb 2018 and Jan 2019 at a continental shelf station (~140m depth) and an open-deep water station.	ACQUITY UPLC system (Waters) equipped with a photodiode array detector and a C8 column (1.7 μm particle size, 2.1 mm internal diameter, 50 mm column length, ACQUITY UPLC BEH, 186002877)	ACQUITY UPLC system (Waters)
Results of incurrent/excurrent water samples taken from three sponge species from the Florida Keys (USA) over two or three days using a modified version of the vacuSIP in January 2022	Targeted metabolomics were measured using the ultra-performance liquid chromatography (Accela Open Autosampler and Accela 1250 Pump, Thermo Scientific) coupled to a heated electrospray ionization source (H-ESI) and a triple quadrupole mass spectrometer (TSQ Vantage, Thermo Scientific) operated under selected reaction monitoring (SRM) mode at the Woods Hole Oceanographic Institution (WHOI).	ultra-performance liquid chromatography (Accela Open Autosampler and Accela 1250 Pump, Thermo Scientific)
Effects of sponge excurrent seawater on coral reef picoplankton composition in the Florida Keys January 2022	Targeted metabolomics were measured using the ultra-performance liquid chromatography (Accela Open Autosampler and Accela 1250 Pump, Thermo Scientific) coupled to a heated electrospray ionization source (H-ESI) and a triple quadrupole mass spectrometer (TSQ Vantage, Thermo Scientific) operated under selected reaction monitoring (SRM) mode at the Woods Hole Oceanographic Institution (WHOI).	Accela Open Autosampler and Accela 1250 Pump, Thermo Scientific
Dissolved organic matter and nutrient data from water samples collected in Dec 2020 from six sponge species at two coral reef sites in the Florida Keys	Untargeted metabolomics were measured using the ultra-high performance liquid chromatography system (Vanquish UHPLC, Thermo Scientific™) coupled with an Orbitrap Fusion Lumos Tribid mass spectrometer (Thermo Scientific™).	Vanquish UHPLC
Dissolved organic matter and nutrient data from water samples collected in Dec 2020 from six sponge species at two coral reef sites in the Florida Keys	Targeted metabolomics were measured using the ultra-performance liquid chromatography (Accela Open Autosampler and Accela 1250 Pump, Thermo Scientific) coupled to a heated electrospray ionization source (H-ESI) and a triple quadrupole mass spectrometer (TSQ Vantage, Thermo Scientific) operated under selected reaction monitoring (SRM) mode at the Woods Hole Oceanographic Institution (WHOI).	Accela Open Autosampler and Accela 1250 Pump, Thermo Scientific
Targeted metabolomics data from coral and sea anemone larvae exposed to normoxic or hypoxic conditions in lab and field experiments performed in USA and Bermuda during 2023-2024	Quantitation of metabolites was achieved using multiple reaction monitoring of calibration solutions and study samples on an Agilent 1290 Infinity UHPLC/6495 triple quadrupole mass spectrometer at the University of Pennsylvania Metabolomics Core (RRID:SCR_022381). Raw data were processed using Mass Hunter quantitative analysis software.	Agilent 1290 Infinity UHPLC
Metabolomic data collected during a thermal stress experiment using reef building corals collected in Kāne'ohe Bay, O'ahu, Hawai'i.		Vanquish Horizon UHPLC system (Thermo Fisher Scientific, Waltham, MA)
Untargeted metabolomic data collected during a thermal stress experiment using reef building corals collected in Kāne'ohe Bay, O'ahu, Hawai'i.		anquish Horizon UHPLC system (Thermo Fisher Scientific, Waltham, MA)
Surface water and N.digitalis inhalent/exhalent water sample nutrient and bacterioplankton metadata of Looe Key and Wonderland Reef conducted in 10-15 December 2023	Targeted metabolomics were measured using the ultra-performance liquid chromatography (Accela Open Autosampler and Accela 1250 Pump, Thermo Scientific) coupled to a heated electrospray ionization source (H-ESI) and a triple quadrupole mass spectrometer (TSQ Vantage, Thermo Scientific) operated under selected reaction monitoring (SRM) mode at the Woods Hole Oceanographic Institution (WHOI).	Accela Open Autosampler and Accela 1250 Pump, Thermo Scientific)