Project: Characterization of subsurface extracellular enzymes and the organisms that produce them using metatranscriptomics and bottom-up metaproteomics

Acronym/Short Name:Sediment Metaproteomics
Project Duration:2018-06 -2019-05
Geolocation:White Oak River Estuary sediments 34 44.490'N, 77 07.44W

Description

Project description from C-DEBI (https://www.darkenergybiosphere.org/award/characterization-of-subsurface-extracellular-enzymes-and-the-organisms-that-produce-them-using-metatranscriptomics-and-bottom-up-metaproteomics/)

Approximately 150 Pg of organic carbon resides in subsurface sediments (Ciais et al. 2013). Understanding the preservation, transformation, and remineralization of this carbon is a prerequisite to understanding the global carbon cycle. Extracellular enzymes are required by subsurface heterotrophic microbes to access complex molecules from this reservoir (Arnosti 2011) and thus, influence carbon turnover. Identifying which microbes produce which extracellular enzymes and under what conditions has remained a challenge. Here, it is proposed to apply bottom-up metaproteomics, metatranscriptomics, and shotgun-metagenomics on subsurface microbe communities from the White Oak River estuary to help close this knowledge gap.

This project was funded by a C-DEBI graduate fellowship.


No datasets

People

Lead Principal Investigator: Taylor Royalty
University of Tennessee Knoxville (UTK)

Contact: Andrew Steen
University of Tennessee Knoxville (UTK)


Programs

Center for Dark Energy Biosphere Investigations [C-DEBI]


Data Management Plan

C-DEBIDataManagementPlan_2015.pdf (87.30 KB)
02/09/2025