Pleurochrysis carterae CCMP645 cells in mid-exponential growth phase were transferred (10% v/v) into flasks containing either F/2 (Guillard, 1975) or L1 (Guillard and Hargraves, 1993) media, in duplicate. Host abundance in each flask was monitored by flow cytometry (FCM) on a BD FACScan(TM) System for a 106-day period. The frequency of sampling varied throughout the period of the study as indicated in the excel data file. At each sampling time point 1 ml volume sample was collected from each flask and preserved in 0.5% paraformaldehyde, then snap frozen in liquid nitrogen and stored at -80C until ready for FCM analysis. Flow cytometry sample acquisition time was one minute for all samples. FCM analysis was carried out as described in Marie et al. 2005.
References
Guillard, Robert RL. "Culture of phytoplankton for feeding marine invertebrates." Culture of marine invertebrate animals. Springer US, 1975. 29-60.
Guillard, R. R. L., and P. E. Hargraves. "Stichochrysis immobilis is a diatom, not a chrysophyte." Phycologia 32.3 (1993): 234-236. https://doi.org/10.2216/i0031-8884-32-3-234.1
Marie, Dominique, Nathalie Simon, and Daniel Vaulot. "Phytoplankton cell counting by flow cytometry." Algal culturing techniques (2005): 253-267.
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