Dataset: bacteria_FCM_lab
Data Citation:
Landry, M. (2014) Heterotrophic (non-pigmented) bacteria abundances determined by flow cytometry (post-cruise/on-shore analysis) from samples collected on R/V Melville cruise MV1008 in the Costa Rica Dome in 2010 (CRD FLUZiE project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2014-05-30 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.516204.1 [access date]
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This dataset is licensed under Creative Commons Attribution 4.0.
If you wish to use this dataset, it is highly recommended that you contact the original principal investigators (PI). Should the relevant PI be unavailable, please contact BCO-DMO (info@bco-dmo.org) for additional guidance. For general guidance please see the BCO-DMO Terms of Use document.
DOI:10.1575/1912/bco-dmo.516204.1
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Spatial Extent: N:10.2998 E:-86.735 S:6.6219 W:-92.9871
Temporal Extent: 2010-06-24 - 2010-07-24
Project:
Costa Rica Dome FLUx and Zinc Experiments
(CRD FLUZiE)
Program:
Principal Investigator:
Michael R. Landry (University of California-San Diego, UCSD-SIO)
Contact:
Karen E. Selph (University of Hawaiʻi at Mānoa, SOEST)
BCO-DMO Data Manager:
Shannon Rauch (Woods Hole Oceanographic Institution, WHOI BCO-DMO)
Version:
1
Version Date:
2014-05-30
Restricted:
No
Validated:
Yes
Current State:
Final no updates expected
Heterotrophic (non-pigmented) bacteria abundances determined by flow cytometry (post-cruise/on-shore analysis) from samples collected on R/V Melville cruise MV1008 in the Costa Rica Dome in 2010 (CRD FLUZiE project)
Abstract:
Non-pigmented bacteria abundances from preserved (0.5% paraformaldehyde) frozen samples run on a Beckman-Coulter Altra flow cytometer after staining with Hoechst 33342 DNA stain and excitation with 200 mW UV and 1W 488 nm laser light. Bacteria discriminated based on DNA content (450±40 nm), as well as forward and 90° side light scatter parameters. Samples were collected on the MV1008 cruise in the Costa Rica Dome (CRD) region of the Eastern Tropical Pacific Ocean.