Dataset: brooded coral larvae 3 - carbonate chemistry

Name: Seawater carbonate chemistry from experiment on brooded coral larval, March 2011, Taiwan (Cumbo et al, JEMBE, 2013) (MCR LTER & Climate Coral Larvae projects)
Published: 2014-10-07
Keywords: oceans

Cite This Dataset

Spatial Extent: N:21.93817 E:120.74602 S:21.93817 W:120.74602

Temporal Extent: 2011-03-02 - 2011-03-18

Project:

Moorea Coral Reef Long-Term Ecological Research site (MCR LTER)

The ecophysiological basis of the response of coral larvae and early life history stages to global climate change (Climate_Coral_Larvae)

Program:

Long Term Ecological Research network (LTER)

Principal Investigator:

Peter J. Edmunds (California State University Northridge, CSUN)

Co-Principal Investigator:

Dr Vivian R Cumbo (California State University Northridge, CSUN)

Tung-Yung Fan (National Museum of Marine Biology and Aquarium, NMMBA)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2014-10-07

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Seawater carbonate chemistry from experiment on brooded coral larval, March 2011, Taiwan (Cumbo et al, JEMBE, 2013) (MCR LTER & Climate Coral Larvae projects)

Abstract:

The physiological development of brooded larvae from the pocilloporid corals Pocillopora damicornis in southern Taiwan under elevated temperature and pCO2 was examined. These data include seawater carbonate chemistry from experiments on the physiological development of brooded coral larvae conducted in March 2011. Reported parameters include temperature, salinity, pH, total alkalinity, pCO2, bicarbonate and carbonate concentration, and the saturation state (omega) for aragonite. These data were published in Cumbo et al, JEMBE, 2013.

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Methods & Sampling

Treatments were created in eight 150 L tanks, each filled with 120 L of filtered (1 um) seawater that was changed partially (20%) every day (at ~17:00 h). Tanks were individually heated (300 Wheaters, Taikong Corporation) and chilled (Aquatech Ac11 or Shyeh Duwai Enterprise), with the temperature regulated using programmable, digital controllers (±0.1 °C, AquaControllers, Neptune Systems). Illumination was provided by metal halide (Phillips 150 W 10,000 k) and fluorescent (39 W, Phillips T5 460 nm) bulbs to create a mean light intensity of 268± 17 µmol quanta m-2 s-1 (±SE, n=64). The light intensity was selected to approximate that found at the collection depth of the parent colonies in March.

Treatments were created by blending CO2 with air, and continually assessing the mixture through an Infra Red gas analyzer (S151, Qubit Systems), which dynamically adjusted the flow of CO2 to maintain desired levels. The gas mixture was supplied through an air stone to four of the eight tanks, with others supplied with compressed air (i.e., ambient pCO2). The conditions in the tanks were analyzed for pH, salinity, temperature, and total alkalinity (TA) using standard operating procedures (Dickson et al., 2007), and the program CO2SYS (Lewis and Wallace, 1998) to calculate DIC parameters. The gas mixing technology and the methods for seawater analyses essentially are identical to those we have used before (see Dufault et al., 2012; Edmunds, 2011). In the present analysis, the calculated TA values of certified reference materials supplied by Dr. Andrew Dickson, Scripps Institute of Oceanography (batch no. 98 and 107), were determined within a mean of 1% of the certified value.

The incubation system created target temperatures of ~24.00 °C versus 30.50 °C, and target pCO2 values of ambient versus 86.1 Pa, and an irradiance of ~268 µmol quanta m-2 s-1. Conditions in the tanks were measured at least daily using a certified digital thermometer (Model 15-077-8, Fisher Scientific,±0.05 °C), a cosine-corrected quantum light meter (Li-Cor LI-192 attached to an LI-1400), and a sample ofwater withdrawn from each tank for pH, salinity, and TA analysis.

The 'ambient' and 'high' pCO2 levels: 49.4 Pa versus 86.2 Pa
The 'ambient' and 'high' temperatures: 24.00 °C [ambient] versus 30.49 °C

Data also available from PANGAEA: doi:10.1594/PANGAEA.823582

Data Processing Description

BCO-DMO processing notes:

- added conventional header with dataset name, PI name, version date, reference information
- renamed parameters to BCO-DMO standard
- added lab, lat, lon columns
- columns not served: temp out, press out, pH out, fugacity, CO2 out, B Alk out, OH out, P Alk out Si Alk out, Revelle, Omega-Ca, xCO2 out.
- reformatted date from m.d.yyyy to yyyy-mm-dd
- removed "Tank " from data in tank column

Data Files

File
brood3_chem.csv (Comma Separated Values (.csv), 3.41 KB) MD5:1a91974447bd440798d22ba04dc2a513 Primary data file for dataset ID 535163

Supplemental Files

File
Biological data for "brooded coral larvae expt. 3" datasets filename: Cumbo_etal_2012_JEMBE_data1_BCODMO.xls (Octet Stream, 154.50 KB) MD5:e6c5e6012df9bfc581b9f769f7e52a98 Original biological data for Cumbo et al 2013 including respiration raw data, respiration by mg protein, symbiont densities, protein content, % mortality
Tank physical data filename: Cumbo_etal_2012_JEMBE_Tank_Parameters_BCODMO.xlsx (Octet Stream, 57.05 KB) MD5:0170402805d7c1fe4784a51d2b26fb66 Tank physical data for "brooded coral larvae 3" experiment including seawater chemistry, light and temperature data.

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-0844785

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
lab_Edmunds_NMMBA		2010-03-18	Natl Museum Mar. Bio. and Aquar. Taiwan	Peter J. Edmunds (Lead Principal Investigator)	data info

Instruments

Aquarium chiller

Supplied Description:

Aquatech Ac11 or Shyeh Duwai Enterprise

Instrument Type

Generic Name: Aquarium chiller

Generic Description:

Immersible or in-line liquid cooling device, usually with temperature control.

Gas Analyzer

Supplied Description:

Infra Red gas analyzer (S151, Qubit Systems)

Instrument Type

Generic Name: Gas Analyzer

Generic Description:

Gas Analyzers - Instruments for determining the qualitative and quantitative composition of gas mixtures.

Immersion heater

Supplied Description:

300 Wheaters, Taikong Corporation

Instrument Type

Generic Name: Immersion heater

Generic Description:

Submersible heating element for water tanks and aquaria.

LI-COR LI-192 PAR Sensor

Supplied Name: LI-COR LI-192 light sensor

Supplied Description:

cosine-corrected quantum light meter (Li-Cor LI-192 attached to an LI-1400)

Instrument Type

Generic Name: LI-COR LI-192 PAR Sensor

Acronym: LI-COR LI-192 PAR

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0120/

Generic Description:

The LI-192 Underwater Quantum Sensor (UWQ) measures underwater or atmospheric Photon Flux Density (PPFD) (Photosynthetically Available Radiation from 360 degrees) using a Silicon Photodiode and glass filters encased in a waterproof housing. The LI-192 is cosine corrected and features corrosion resistant, rugged construction for use in freshwater or saltwater and pressures up to 800 psi (5500 kPa, 560 meters depth). Typical output is in um s-1 m-2. The LI-192 uses computer-tailored filter glass to achieve the desired quantum response. Calibration is traceable to NIST. The LI-192 serial numbers begin with UWQ-XXXXX. LI-COR has been producing Underwater Quantum Sensors since 1973.

These LI-192 sensors are typically listed as LI-192SA to designate the 2-pin connector on the base of the housing and require an Underwater Cable (LI-COR part number 2222UWB) to connect to the pins on the Sensor and connect to a data recording device.

The LI-192 differs from the LI-193 primarily in sensitivity and angular response.
193: Sensitivity: Typically 7 uA per 1000 umol s-1 m-2 in water. Azimuth: < ± 3% error over 360° at 90° from normal axis. Angular Response: < ± 4% error up to ± 90° from normal axis.
192: Sensitivity: Typically 4 uA per 1000 umol s-1 m-2 in water. Azimuth: < ± 1% error over 360° at 45° elevation. Cosine Correction: Optimized for underwater and atmospheric use.
(www.licor.com)

Water Temperature Sensor

Supplied Name: Water Temp Sensor

Supplied Description:

certified digital thermometer (Model 15-077-8, Fisher Scientific,±0.05 °C)

Instrument Type

Generic Name: Water Temperature Sensor

Acronym: Water Temp Sensor

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/134/

Generic Description:

General term for an instrument that measures the temperature of the water with which it is in contact (thermometer).

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
lab	laboratory	unitless	laboratory
lat	latitude; north is positive	decimal degrees	lat
lon	longitude; east is positive	decimal degrees	lon
date	local date	yyyy-mm-dd	date
tank	tank id number	tank	tank
treatment	temperature: AT=ambient (24 C) or HT=high (30.49 C); pCO2 treatment: AC=ambient (419-470 uatm) or HC=high (604-742 uatm)	unitless	treatment
sal	salinity	PSU scale	sal
treatment_temp	target temperature	degrees Celsius	temp
temp_tank	temperature reading in the tank	degrees Celsius	temp
TA	total alkalinity	umol/kg	TALK
pH	pH: The measure of the acidity or basicity of an aqueous solution	pH scale	pH
pCO2	partial pressure of carbon dioxide by computation from pH and alkalinity	uatm	pCO2
bicarbonate	concentration of bicarbonate ion ([HCO3]-) in seawater	umol/kg seawater	bicarbonate
carbonate	concentration of carbonate ion ([CO3]2-)	umol/kg seawater	carbonate
omega_Ara	the saturation state of seawater with respect to aragonite	unitless	OM_ar

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Dataset: brooded coral larvae 3 - carbonate chemistry