Dataset: Synechococcus Si uptake: phosphorous

Name: Synechococcus silica uptake rates from cultures with and without added orthophospate from laboratory experiments at the Dauphin Island Sea Lab from August of 2013 to September of 2014 (Si_in_Syn project)
Published: 2017-12-04
License: http://creativecommons.org/licenses/by/4.0/
Keywords: oceans

Cite This Dataset

Spatial Extent: N:41.19 E:-65.6 S:22.495 W:-124.1668

Project:

Understanding the Role of Picocyanobacteria in the Marine Silicate Cycle (Si_in_Syn)

Principal Investigator:

Dr Jeffrey W Krause (Dauphin Island Sea Lab, DISL)

Co-Principal Investigator:

Dr Mark Brzezinski (University of California-Santa Barbara, UCSB)

Contact:

Dr Jeffrey W Krause (Dauphin Island Sea Lab, DISL)

BCO-DMO Data Manager:

Amber York (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version Date:

2017-12-04

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Data URL:

https://www.bco-dmo.org/dataset/674286/data

Synechococcus silica uptake rates from cultures with and without added orthophospate from laboratory experiments at the Dauphin Island Sea Lab from August of 2013 to September of 2014 (Si_in_Syn project)

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Description

This dataset contains silica uptake rates from Synechococcus cyanobacteria cultures grown in Sargasso Sea water with and without added orthophosphate. Experiments took place at the Krause Lab at Dauphin Island Sea Laboratory (30.2501,-88.0788) between August of 2013 to September of 2014.

These results are also presented in the following paper:

Brzezinski, M. A., Krause, J. W., Baines, S. B., Collier, J. L., Ohnemus, D. C. and Twining, B. S. (2017), Patterns and regulation of silicon accumulation in Synechococcus spp.. J. Phycol., 53: 746–761. doi:10.1111/jpy.12545

Acquisition Description

Monocultures of four Synechococcus clones (1333, 1334, 2515, 2370) were used to examine variation and controls on Si quotas and rates of Si accumulation. Cultures were procured from the National Center for Marine Algae and Microbiota (NCMA) at the Bigelow Laboratory for Ocean Sciences in Boothbay Harbor, Maine. Many of these clones are also available in other culture collections and have various strain names; here we will refer to each by their NCMA strain number (a.k.a. CCMP number).

Cultures were kept in surface Sargasso seawater (ambient concentrations ~1 μM Si(OH)4, ~0.1 uM PO4) with added orthophosphate (f/2 level, 36 μM) as well as the same seawater without added orthophosphate.

Each incubation was terminated by filtration and processed for measurement of 32Si activity following Krause et al. (2011). After aging for 120 days, secular equilibrium between 32Si and its daughter 32P was achieved and 32Si activity was determined using gas-flow proportional counting using GM 25-5 multicounters (Risø National Laboratory, Technical University of Denmark).

Full details of culturing and experimental methods are described in Brzezinski et al. (in review). (as of 05 Jan 2017)

Processing Description

BCO-DMO Data Manager Processing Notes:
* added a conventional header with dataset name, PI name, version date
* modified parameter names to conform with BCO-DMO naming conventions
* blank values replaced with no data value 'nd'
* latitude and longitude of Dauphin Island Sea Lab added to dataset
* Si uptake rate limited to 5 decimal places

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1335012

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
Krause_DISL_2013-2015		2013-03-01	lab Dauphin_Island_Sea_Lab		data info

Instruments

GM multicounter

(more info)

Short Name:

Generic Description

A gas flow multicounter (GM multicounter) is used for counting low-level beta doses. GM multicounters can be used for gas proportional counting of 32Si to 32P. For more information about GM multicounter usage see Krause et. al. 2011 .

PI supplied instrument name: GM 25-5 multicounter

Dataset-specific description

Manufactured by Riso Laboratories

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
clone_id	Synechococcus clone identifier (NCMA strain and CCMP number)	unitless	sample
clone_lat	Latitude of the clone collection site	decimal degrees	lat
clone_lon	Longitude of the clone collection site	decimal degrees	lon
treatment	Shorthand code for additions to culture medium (e.g. N means added nitrate)	unitless	treatment
uptake_rate	Silica uptake rate	reciprocal hours (h-1)	Si
treatment_descrip	Description of what additions were made to culture medium	untiless	treatment

Database

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Dataset: Synechococcus Si uptake: phosphorous