Bugula neritina colonies were collected by hand off the sides of floating docks around Morehead City, NC (latitude: 34.7, longitude: -76.8). Larvae from individual colonies were collected and settled into 10 mL of filtered seawater with (treated) or without (control) 100 ug/mL of gentamicin. Larvae were allowed to settle overnight. The control and treated seawater was changed the next morning for the next 3 days. The plates were transported to Georgia Tech where they were placed in 2.5 gallon tanks with artificial seawater maintained in environmental chambers at 16 degrees C and 22 degrees C. They were fed ad libitum an artificial diet of the phytoplankton Rhodomonas lens for approximately one month. After the 1-month period, the size of the colonies was measured by counting the number of bifurcations in each colony. A Leica stereomicroscope was used while counting the number of colony bifurcations. Samples of larvae, juvenile, and the grown colonies were placed in RNAlater for symbiont quantification analysis by quantitative PCR (currently in progress).