Dataset: Sea star sample information: Dutch Harbor, AK

Name: Sea star sample information from Dutch Harbor, AK from September 12-14th, 2015 (Sea Star Microbiology project)
Published: 2017-10-13
Keywords: biota, oceans

Cite This Dataset

Spatial Extent: N:53.902823 E:-166.465081 S:53.856984 W:-166.5631

Temporal Extent: 2015-09-12 - 2015-09-14

Project:

Microbial ecology of sea star wasting disease (Sea Star Microbiology)

Principal Investigator:

Ian Hewson (Cornell University, Cornell)

Contact:

Ian Hewson (Cornell University, Cornell)

BCO-DMO Data Manager:

Amber D. York (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version Date:

2017-10-13

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Sea star sample information from Dutch Harbor, AK from September 12-14th, 2015 (Sea Star Microbiology project)

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Description

This dataset includes sea star sampling information collected for a survey of sea star-associated densovirus (SSaDV) prevalence and load in asymptomatic animals from near Dutch Harbor, Unalaska, AK from September 12-14th, 2015. The data include sample number, species, size, collection time, latitude, and longitude.

Methods & Sampling

Methodology: A previous survey (March 2015, before currently funded work) established that among 66 asteroids collected from Dutch Harbor, AK, none were positive for SSaDV. We again surveyed this population during aquarium specimen collection in September 2015 to determine the presence/load of SSaDV.

Sampling and analytical procedures: Animals were collected by hand or by SCUBA diver at 3 locations near Dutch Harbor, AK in September 2015. Body wall tissues were biopsied on site to determine the presence/load of SSaDV. Biopsy punches were immediately placed in 100% EtOH before transport back to the lab at Cornell University. At the same time, 68 live asteroids (n = 8 Pycnopodia helianthoides, n = 20 Solaster stimpsonii, n = 20 Evasterias troscheli, n = 20 Crossaster papposus) were collected by SCUBA diver at Dead Man’s Bend, placed into shipping containers, and then sent back to the lab at Cornell University. Unfortunately, all except 6 Evasterias troscheli and 3 Solaster stimpsonii died in transit due to Alaska Airlines and Fedex delays. Size was measured by measuring the diameter of the individual (longest arm to longest arm) visually using a ruler.

SSaDV abundance/load will be determined by qPCR following the approach of Hewson et al., 2014 (PNAS).

Data Processing Description

BCO-DMO Data Manager Processing Notes:
* added a conventional header with dataset name, PI name, version date
* modified parameter names to conform with BCO-DMO naming conventions

Data Files

File
DutchHarbor.csv (Comma Separated Values (.csv), 1.92 KB) MD5:ce5e0f0d37727c8beedcfb2b7307b40d Primary data file for dataset ID 719906

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1537111

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
SeaStarMicrobiology_Hewson			shoreside Alaska	Ian Hewson (Principal Investigator)	data info

Instruments

None available yet

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Sample_No	Sample identifier	unitless	sample
Location	Location name	unitless	site
Site	Site name at location	unitless	site
Species	Species name	unitless	species
Size	Diameter of the individual (longest arm to longest arm) visually using a ruler.	centimeters (cm)	length
Depth	Collection depth	meters (m)	depth
Date_Collected	Collection date in format yyyy-mm-dd	unitless	date
Lat	Collection latitude	decimal degrees	lat
Long	Collection longitude	decimal degrees	lon

Status message

Database

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Dataset: Sea star sample information: Dutch Harbor, AK