Dataset: Pseudo-nitzschia diversity and viral titers

Samples were collected on foot from shore. Temperature and salinity were measured with a handheld YSI device. Nutrients were measured at the UW Marine Chemistry Lab following the protocols of the WOCE Hydrographic Program using a Seal Analytical AA3.

Approximately 15 L of surface water was filtered through triplicate 3.0 and 0.2 μm 147 mm polyethersulfone filters (Millepore) with a peristaltic pump within 2 h of sampling. The filters were frozen at −80◦C for molecular analysis. The viruses in the filtrate were precipitated by adding iron chloride (1 g L− 1 ) and incubating for 12 h at 13◦ C in the dark.

Pseudo-nitzschia species diversity was assessed by extracting DNA from the 3.0 μm filters and performing ARISA with Pseudo-nitzschia-specific primers. Abundances are expressed as a percent of the total Pseudo-nitzschia community detected.

Viral titer was assessed by incubating viral communities concentrated from each sample in a series of 10-fold dilutions of with 8 independent Pseudo-nitzschia isolates. Cultures were monitored for death via chlorophyll-a fluorescence. The infectious units were determined
based on MPN tables.

Full details of these methods are in Carlson et al. (2016).

Location:
Puget Sound: Penn Cove, Washington (48.2397, −122.6795) and
Washington coast: Grays Harbor, Washington (46.7625, −124.0898)