Dataset: Alcanivorax sp. 97CO-6

Project accession number
PIXQ01000000

Species name
Alicanivorax sp. 97CO-6

Description of types of sequences:
The draft genome of strain 97CO-6, which contains 3,253,423 bp, with a G+C content of 54.53%, as well as 2,931 protein-coding genes and 42 tRNAs.

Locations where species were collected
Alcanivorax sp. Strain 97CO-6 was isolated from a crude oil-consuming bacterial consortium, enriched from Yellow Sea sediments from China

Sequencing and analysis methods
Whole-genome shotgun sequencing of the Alcanivorax sp. 97CO-6 genome was done using Solexa paired-end sequencing technology by Shanghai Majorbio Bio-Pharm Technology Co., Ltd (Shanghai, China). A total of 13,445,638 paired-end reads (180-bp and 800-bp libraries) were generated to reach a 398-fold depth of coverage using the Illumina HiSeq 2000 platform (Illumina, Inc., San Diego, CA, USA). The reads were assembled using SOAPdenovo version 1.05. The resulting genome sequence of Alcanivorax sp. 97CO-6 comprises  43 contigs (N90 = 78,739), with 3,253,423 bp and an average G+C content of 54.53%. Gene annotation was carried out using the NCBI Prokaryotic Genome Annotation Pipeline (PGAP) (https://www.ncbi.nlm.nih.gov/genomes/static/Pipeline.html), which was followed by manual editing. The annotation of the genome includes 2,931 candidate protein-encoding genes (with an average size of 960 bp), yielding a coding intensity of 86.50%. A total of 2,118 proteins could be assigned to clusters of orthologous (COG) groups; 42 tRNAs for all 20 amino acids and one 16S-23S-5S rRNA operon were identified using tRNAscan version 1.23 (8) and rRNAmmer version 1.2 (9), respectively.

All of the above information is from the published article “Genome Sequence of a Marine Alkane Degrader, Alcanivorax sp. Strain 97CO-6.” Xiafei Yin, Xiao Luan, Angela Xu, Qian Li, Zhisong Cui, and David L. Valentine, Genome Announcements 2018 6 (8): e00087-18 doi: 10.1128/genomeA.00087-18