Dataset: FeOB oxygen consumption pyrrhotite

These data were collected by placing each strain in a 100 mL serum vial with 6 mL of their standard, published media with 200 mg pyrrhotite powder as a source of Fe(II).  The headspace was filled with a gas mix of 8% oxygen/10% carbon dioxide/82% nitrogen by using bottled gas mixes and a regulator to flush the headspace without over pressurization.  Prior to sealing the serum vials, a Presens OPTODE dot (sensor) was placed inside the vial, allowing non-invasive gas sampling of the changes in O2 in the headspace.  A Presens four channel system was used to measure changes in oxygen concentration in real-time in each bottle.  A total of four channels were measured during each experiment: channels 1 through 3 are the biological treatments and channel 4 was a kill control (microbes were by placing on a heat block at 100 degrees C for 5 minutes). After 14 days of incubation at 20˚C, vial headspace were replaced with the original starting gas mix. Incubation then continued for 7 more days.