Dataset: AT37-06 Sediment Geochemistry Summary

Location: Guaymas Basin, Gulf of California, 27 00.00 N, -111 20.00 W

Sediment samples were collected by the DSV Alvin using PVC push cores.  Upon arrival at the surface the cores were described and cataloged prior to being sectioned into discrete depth intervals.  Porewater was separated from the sediment by centrifugation.  Porewater and sediment samples were preserved and analyzed as follows: 

1) Nutrients (DOC, TDN, NOx, NO2, NH4, PO4, TDP): Porewater sample was filtered through a pre-rinsed 0.2 um regenerated cellulose Target2 syringe filter (Thermo Scientific, Prod. No. F25047), collected into an HDPE bottle and stored frozen at -20°C until analysis.  Individual analytes were analyzed as follows:
DOC was determined using high temperature catalytic combustion and an NDIR detector following the method described in Sugimura and Suzuki, 1988.  
TDN was determined using high temperature combustion and a chemiluminescence detector following the method described in Watanabe et. al, 2007.
NOx was determined using chemical reduction and a nitric oxide detector following the method described by Garside, 1982.  
NO2 was determined using the colorimetric method described by Bendschneider and Robinson, 1952 as reproduced by Parsons, Marta, and Lalli, 1984.
NH4 was determined using the colorimetric method described by Solorzano, 1969.
PO4 was determined using the colorimetric method described by Strickland and Parsons, 1972.
TDP was determined using the colorimetric method described by Solorzano and Sharp, 1980.

2) C1-C3 (CH4, C2H6, C3H8): Sediment sample was collected into a glass serum vial, preserved with 1M NaOH, crimp-sealed with a butyl rubber stopper and stored at room temperature until analysis.  C1-C3 was determined by headspace analysis using an SRI 8610C gas chromatograph equipped with a flame ionization detector and SRI Hayesep D 6’x1/8” column (Prod. No. 8600-PKDB).

3) H2S: Porewater sample was collected into a 15 mL centrifuge tube containing 2M zinc acetate and stored at 5°C until analysis.  H2S was determined using the colorimetric method described by Cline, 1969.

4) SO4: Porewater sample was collected into a 7 mL scintillation vial, acidified with 10 uL concentrated hydrochloric acid per mL sample and sealed with a PTFE lined cap.  SO4 determination was made after Cl removal using a Dionex OnGuard Ag cartridge (Prod. No. 039637).  Sample analysis was performed using KOH eluent supplied by a Dionex EGC III KOH Eluent Generator Cartridge (Prod. No. 074532), Dionex CR-ATC Continuously Regenerated Trap Column (Prod. No. 060477), Dionex AERS 500 Electronically Regenerated Suppressor (Prod. No. 082541), Dionex IonPac AG19 Guard Column (Prod. No. 062888), Dionex IonPac AS19 Analytical Column (Prod. No. 062886) and Dionex CRD 200 RFIC Carbonate Removal Device (Prod. No. 062986).

5) TPN: The pressed sediment sample (mud-cake) was dried at 60°C and then homogenized via grinding. TPN was determined by the method described by Gordan, Jr., 1969 as reproduced by Sharp, 1974. Samples were analyzed on a ThermoFinnigan FlashEA 1112 series NC Soil Analyzer. 

6) TPC: The pressed sediment sample (mud-cake) was dried at 60°C and then homogenized via grinding. TPC was determined by the method described by Gordan, Jr., 1969 as reproduced by Sharp, 1974. Samples were analyzed on a ThermoFinnigan FlashEA 1112 series NC Soil Analyzer. 

7) POC: The pressed sediment sample (mud-cake) was acidified with 1 N HCl, dried at 60°C, and then homogenized via grinding. POC was determined by the method described by Gordan, Jr., 1969 as reproduced by Sharp, 1974. Samples were analyzed on a ThermoFinnigan FlashEA 1112 series NC Soil Analyzer. 

8) Calculated Values (NO3, DIN, DON, DOP):  These values were calculated as follows:
NO3 = NOx - NO2
DIN = NOx + NH4
DON = TDN - DIN
DOP= TDP - PO4