Dataset: C and N isotopes

Name: Carbon and Nitrogen isotopes in an experimental benthic chamber, Maunalua, O’ahu, Hawai’i, 2015-2016
Published: 2020-08-27
Keywords: macroalgae, Productivity, isotope enrichment, coral reef, invasive algae, phytoplankton, oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.822239.1

Spatial Extent: N:21.275824 E:-157.760472 S:21.27273 W:-157.76335

Temporal Extent: 2015-10-09 - 2016-11-03

Project:

Collaborative Research: Dissolved organic matter feedbacks in coral reef resilience: The genomic & geochemical basis for microbial modulation of algal phase shifts (Coral DOM2)

Principal Investigator:

Craig E. Nelson (University of Hawaiʻi at Mānoa, HIMB)

Contact:

Florybeth F. La Valle (University of Hawaiʻi at Mānoa, HIMB)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2020-08-27

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Carbon and Nitrogen isotopes in an experimental benthic chamber, Maunalua, O’ahu, Hawai’i, 2015-2016

Abstract:

Carbon and Nitrogen isotopes in an experimental benthic chamber, Maunalua, O’ahu, Hawai’i, 2015-2016

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Archival Copy

Version Date	Archive	Persistent Identifier (PID)	Date PID Issued
2020-08-27	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.822239.1	2020-09-09

Description

This dataset carbon and nitrogen isotope ratios from experiments conducted at the University of Hawaii, Manoa in 2015.

Experiment CRANE (Coral Reef Acclimation to Nutrient Enrichment) identifies a month-long mesocosm incubation study designed to understand the response of the coral reef community to long-term nutrient exposure.

Methods & Sampling

Parameter
Method

Final_mass_algae_g
All of the algal biomass was taken from the benthos covered by the benthic chamber at the end of the experiment. Algae was taken back to the lab, sorted by species, cleaned of sediment and epiphytes with DI water. Algae was dried at 60 degrees Celsius for 3 days and a final dry weight was taken by species.

L_filtered
0.5-1.14L of seawater was taken out of the benthic chamber and filtered through a 0.2 um previously combusted glass fiber filter.

Weight_mg
Sample is weighed during processing using a microbalance.

C, N
Algal and phytoplankton samples were dried for 3 days at 60 degrees Celsius. Analysis of samples for carbon [ug C] and nitrogen [ug N], and isotopic composition [d13C (permil vs. PDB) and d15N (permil vs. AIR) ] was done using the following instrumentation: Costech ECS 4010 Elemental Combustion System/Zero Blank Autosampler /ThermoFinnigan MAT Conflo IV/ThermoFinnigan DeltaXP.

Data Processing Description

BCO-DMO Processing:
- modified parameter names to conform with BCO-DMO naming conventions

Data Files

File
cn_isotopes.csv (Comma Separated Values (.csv), 11.21 KB) MD5:8f8671037448b8c6ccd2b7d9aaf52e84 Primary data file for dataset ID 822239

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1538393

Deployments

None available yet

Instruments

Laboratory Autosampler

Supplied Name: Costech ECS 4010 Elemental Combustion System/Zero Blank Autosampler /ThermoFinnigan MAT Conflo IV/ThermoFinnigan DeltaXP

Supplied Description:

For analysis of samples for carbon [_g C] and nitrogen [_g N], and isotopic composition [d13C (â vs. PDB) and d15N (â vs. AIR) ].

Instrument Type

Generic Name: Laboratory Autosampler

Generic Description:

Laboratory apparatus that automatically introduces one or more samples with a predetermined volume or mass into an analytical instrument.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Exp_ID	Identification for experiment; corresponds to Exp ID in La Valle_Wailupe productivity macroalgae and phytoplankton_2015-2016.xlsb	NA	exp_id
sample_id	Identification for isotope sample (algal tissue or phytoplankton on a filer).	NA	sample
species	Macroalgal species or phytoplankton designation	NA	taxon
Ambient_or_Enriched	Ambient refers to a sample taken before the enrichment at the beginning of the experiment. Enriched refers to a sample taken at the end of the experiment.	NA	sample_descrip
Final_mass_algae_g	Final dry weight of algal species in the benthic chamber. No value for phytoplankton.	gram (g)	mass
L_filtered	Sea water volume filtered.	Liter (L)	vol_filt
Weight_mg	weight of sample given by lab	milligram (mg)	weight
ug_N	concentration of nitrogen sample given by lab	micrograms of Nitrogen (ug N)	N
d15N_permil_vs_AIR	delta 15N of sample given by lab; permil vs. air	unitless	d15N
ug_C	concentration of carbon sample given by lab	micrograms of Carbon (ug C)	C
d13C_permil_vs_PDB	delta 13C of sample given by lab; permil vs. PDB	unitless	d13C

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Database

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Dataset: C and N isotopes