Dataset: Experimental_coral_treatments

Corals of three species (Porites lobata, Pocillopora cf. meandrina, and Acropora hyacinthus) were collected on the reef crest (GPS location given in the metadata), fragmented, and allowed to equilibrate for >24 hours at Gump research station in shaded flowing seawater tables. Fragments from a single coral head were exposed to all treatments and indicated by the same replicate letter.  Coral fragments were exposed to slightly elevated bicarbonate (+1 millimolar) and Ammonium (0.5 millimolar NH4) for 24 hours prior to being sampled (T0 controls) and then exposed to to treatments including Control (no modification), Nutrient (2 millimolar NO3), Temperature (+3oC), or coral exudate (2 millimolar dissolved organic carbon + particulate organic carbon created by bleaching co-occurring con-specific coral head).  Corals were kept in individual recirculating water baths. At specified time points (6,12, 24, 48 hours) corals were sacrificed and water from the water baths was sampled for microbial community composition by filtering 0.5 liters of water through a 0.2 micron sterivex filter (Sterivex-GP 0.22 micromolar, SVGP01050, Millipore) after which they were frozen. Coral exudate blank clogged the sterivex and so much less water was filtered. Coral pieces were placed in Zymo DNA/RNA shield. Initial processing of the corals included bead beating and then frozen at -80 and shipped back to either Rice University or Oregon State University (OSU) for extraction using the ZYMO quick-DNA extraction kit.  DNA was extracted using either the Zymo quick-DNA  extraction kit (corals) or the Quigen Powerwater kit (water samples) prior to DNA amplification following manufacturer protocols. DNA was amplified (at OSU) following the Earth Microbiome Project protocols (using the updated primers of 515f (Parada et al. 2016) and 806r (Apprill et al. 2015).  Due to co-amplification of eukaryotic 12S rRNA genes, DNA was size selected using Blue Pippin (Sage Scientific) prior to sequencing to minimize 12S sequencing. While we used dual indexed primers during sequencing, reverse read quality scores were not acceptable and only forward reads were used and uploaded to the SRA.

All data associated with this submission has been made available through the NCBI Short Read Archive under Bioproject PRJNA684406 (https://www.ncbi.nlm.nih.gov/bioproject/PRJNA684406/)

Particular SRA accession numbers are provided in the accompanying CSV file.