Dataset: SRP Bioassay Experiments

Name: Concentrations of soluble reactive phosphate in treatments amended with dissolved organic phosphate compounds in bioassay experiments (incubation 48h) with seawater from R/V Savannah cruise SAV-19-02 in the NW Atlantic Ocean in Spring of 2019
Published: 2021-11-02
Keywords: oceans

View Data: Data not available yet

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Spatial Extent: N:31.7635 E:-79.8421 S:31.0175 W:-80.7965

Temporal Extent: 2019-03-30 - 2019-04-10

Project:

Collaborative Research: Assessing the role of compound-specific phosphorus hydrolase transformations in the marine phosphorus cycle (P-hydrolase)

Principal Investigator:

Solange Duhamel (Columbia University)

Co-Principal Investigator:

Julia Diaz (University of Georgia, UGA)

Contact:

Kahina Djaoudi (University of Arizona, UA)

BCO-DMO Data Manager:

Amber D. York (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2021-11-02

Restricted:

Yes

Release Date:

2022-07-26

Validated:

Current State:

Data not available

Concentrations of soluble reactive phosphate in treatments amended with dissolved organic phosphate compounds in bioassay experiments (incubation 48h) with seawater from R/V Savannah cruise SAV-19-02 in the NW Atlantic Ocean in Spring of 2019

Abstract:

Concentrations of soluble reactive phosphate in treatments amended with dissolved organic phosphate compounds, over an incubation period of 48 h during bioassay experiments with seawater collected during R/V Savannah cruise SAV-19-02 from March to April of 2019 in the Northwestern Atlantic from the surface to 50 m depth.

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Methods & Sampling

Sampling and analytical procedures:

Bioassay experiments were conducted at station 1 and stations 3. At each station, inorganic and organic phosphate amendments were performed on seawater with and without nitrogen enrichment (NH4Cl, NaNO3). Bioassay Experiments consisted in incubating, over an incubation period of 48h, surface seawater (5m) with inorganic or organic phosphate compounds (20 µM; final concentration of P) including, polyphosphate (polyp), inorganic phosphate (Pi), nucleotides (ATP or AMP) and methylphosphonate (Mepn). In each incubation experiment, a control treatment (surface seawater without amendment) was included.

To investigate a potential abiotic hydrolysis of inorganic and organic phosphate compounds, duplicates of surface seawater sampled inshore (5 m) and previously autoclaved (120°C; 30 min), were incubated in parallel. The autoclaved seawater (1L) was amended with either Mepn, AMP, ATP or PolyP (final concentration 20 µM P).

In bioassay experiments, subsamples (50 mL) were taken for SRP determination from each experimental incubation bottle and then filtered through pre-combusted (450°C, 4.5 h) and acid washed (1N HCl) GF/F filter using a syringe (25 mm). The filtrates were then transferred into acid cleaned (10% HCl) HDPE bottles (60 mL) and kept frozen until analysis. SRP concentrations were analyzed following a spectrophotometric method based on the molybdenum reagent (Murphy and Riley, 1962). SRP concentrations (all samples except Pi amended treatments and T=48h of AMP and ATP amended treatments) were analyzed using the Liquid Waveguide Capillary Cell® (LWCC, optical length path=2.5 m). Pi amended treatments as well as T=48h of AMP and ATP amended treatments were analyzed using a conventional spectrophotometer (Genesys®) with a 10 cm cuvette.

Instruments: Measurements of SRP were performed using the Liquid wave guide capillary cell (2.5 m length path, model 3250, World Precision Instrument) and using a conventional spectrophotometer (Genesys®,10 cm cuvette).

Location: Northwestern Atlantic surface waters. Depth: surface-50 m.

Data Processing Description

Data were organized using MATLAB and output as .mat files. Gaps in data were filled with NaN in the .mat files.

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1737083
NSF Division of Ocean Sciences	OCE-2001212
NSF Division of Ocean Sciences	OCE-1736967
NSF Division of Ocean Sciences	OCE-1948042

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
SAV-19-02	Zephyr Zooming in on Enzymatic PhosphoHYdrolysis Reactions	2019-03-30	R/V Savannah	Julia Diaz (Chief Scientist)	data info

Instruments

Liquid Waveguide Capillary Cells

Supplied Name: Liquid wave guide capillary cell (2.5 m length path, model 3250, World Precision Instrument)

Supplied Description:

Measurements of SRP were performed using the Liquid wave guide capillary cell (2.5 m length path, model 3250, World Precision Instrument) and using a conventional spectrophotometer (Genesys®,10 cm cuvette).

Instrument Type

Generic Name: Liquid Waveguide Capillary Cells

Acronym: LWCC

Generic Description:

Liquid Waveguide Capillary Cells (LWCC) are optical sample cells that combine an increased optical pathlength (2-500 cm) with small sample volumes. They can be connected via optical fibers to a spectrophotometer with fiber optic capabilities. Similar to optical fibers, light is confined within the (liquid) core of an LWCC by total internal reflection at the core/wall interface. Ultra-sensitive absorbance measurements can be performed in the ultraviolet (UV), visible (VIS) and near-infrared (NIR) to detect low sample concentrations in a laboratory or process control environment. According to Beer’s Law the absorbance signal is proportional to chemical concentration and light path length.

Spectrophotometer

Supplied Name: Genesys®,10 cm cuvette

Supplied Description:

Instrument Type

Generic Name: Spectrophotometer

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/

Generic Description:

An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples.

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Database

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Dataset: SRP Bioassay Experiments