Dataset: Incomplete sparging

Name: Effects of sample volume on N2O recovery and isotopic analysis (Biological Nitrogen Isotope Fractionation project)
Published: 2021-11-16
Keywords: oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.865053.1

Temporal Extent: 2012-01-31 - 2020-08-24

Project:

CAREER: The biological nitrogen isotope systematics of ammonium consumption and production (Biological Nitrogen Isotope Fractionation)

Principal Investigator:

Julie Granger (University of Connecticut, UConn)

Student:

Mengyang Zhou (University of Connecticut, UConn)

Contact:

Mengyang Zhou (University of Connecticut, UConn)

Analyst:

Bonnie X. Chang (University of Washington, UW)

BCO-DMO Data Manager:

Sawyer Newman (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2021-11-16

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Effects of sample volume on N2O recovery and isotopic analysis (Biological Nitrogen Isotope Fractionation project)

Abstract:

Incomplete N2O analyte recovery during sparging of high-volume samples and associated N and O isotopic ratio offsets.

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Archival Copy

Version Date	Archive	Persistent Identifier	Date Assigned
2021-11-16	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.865053.1	2022-01-14

Methods & Sampling

Methodology:

Sampling and analytical procedures:

 Effects of sample volume on N2O isotope analyses

Crimp-sealed vials (20 mL) containing incremental volumes (0 to 14 mL) of DIW or NO3--deplete seawater were sparged with N2 gas for 30 minutes, aliquoted with N2O gas (10 nmol N) and equilibrated for ≥ 24 hours. The N2O gas in the vials was then extracted, purified and its N and O isotopic composition analyzed on the isotope ratio mass spectrometer. In a parallel set of experiments conducted at Princeton University, referring to Trial “Bonnie 5nmol”, crimp-sealed vials (20 mL) containing incremental volumes (0 to 9.3 mL) of DIW were sparged with helium gas for 30 minutes, aliquoted with N2O gas (5 nmol N) and equilibrated on a shaker for 2 hours. The N2O gas in the vials was then extracted with an autosampler needle that only penetrated samples ≥ 8.3 mL. N2O thus extracted was purified and its N and O isotopic composition analyzed on an isotope ratio mass spectrometer.

Data Processing Description

Processing notes from submitting researcher:

Data were processed using Microsoft Excel

BCO-DMO processing notes

Date formats were changed from mm/dd/yy to yyyy-mm-dd
Spaces and units removed from column headers

Data Files

File
zhou_et_al_lab_data-4.csv (Comma Separated Values (.csv), 11.46 KB) MD5:594cf1ee47d221320b6a40c498083632 Primary data file for dataset ID 865053

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1554474

Deployments

None available yet

Instruments

Gas Chromatograph Mass Spectrometer

Supplied Name: Delta V Advantage and MAT253 continuous flow gas chromatograph isotope ratio mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA)

Supplied Description:

Delta V Advantage and MAT253 continuous flow gas chromatograph isotope ratio mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) interfaced with a modified Thermo Fisher Scientific Gas Bench sample preparation device fronted by dual cold traps (Casciotti et al., 2002) and a GC Pal autosampler (CTC Analytics, Zwingen, Switzerland) - to measure N and O isotope ratio of nitrate using the denitrified method.

Instrument Type

Generic Name: Gas Chromatograph Mass Spectrometer

Generic Description:

Instruments separating gases, volatile substances or substances dissolved in a volatile solvent by transporting an inert gas through a column packed with a sorbent to a detector for assay by a mass spectrometer.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Aliquot	Types of aliquot: freshwater or seawater	unitless	sample_type
Date	Date of the experiments; yyyy-mm-dd Format: %Y-%m-%d	unitless	date
Trial	Trial name	unitless	replicate
GC_IRMS	Gas chromatography combustion isotope ratio mass spectrometry (GC_IRMS) used for isotope analysis: Thermo Delta V at Uconn or Thermo MAT253 GC-IRMS at Princeton	unitless	no_bcodmo_term
N2O_injection	The amount of N2O gas injected	nmol of N	NO2
Aliquot_volume	Volume of aliquot addition	mL	volume
N2O_peak_area	Recoverd N2O peak area measured with a Thermo Delta V GC-IRMS with modified Gas Bench II and a PAL autosampler	Vs	N2O
delta_15N	N isotopic composition of N2O measured with a Thermo Delta V GC-IRMS with modified Gas Bench II and a PAL autosampler	‰ vs. N2Otank	d15N
delta_18O	O isotopic composition of N2O measured with a Thermo Delta V GC-IRMS with modified Gas Bench II and a PAL autosampler	‰ vs. N2Otank	18O

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Database

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Dataset: Incomplete sparging