Dataset: Salinity tolerance without acclimation: Mortality

Name: Salinity tolerance of oysters without acclimation in lab conditions: mortality
Published: 2023-02-24
Keywords: Crassostrea virginica, oyster mortality, salinity acclimation, oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.870210.1

Spatial Extent: N:29.86611 E:-92.03444 S:27.62722 W:-97.23306

Temporal Extent: 2018-11-29 - 2019-04-19

Project:

Collaborative Research: Testing for local adaptation and responses to multiple stressors in populations of eastern oysters inhabiting a natural salinity gradient (Oyster adaptation)

Principal Investigator:

Morgan Kelly (Louisiana State University, LSU)

Jennifer Beseres Pollack (Texas A&M, Corpus Christi, TAMU-CC)

Co-Principal Investigator:

Jerome La Peyre (Louisiana State University, LSU)

Contact:

Jennifer Beseres Pollack (Texas A&M, Corpus Christi, TAMU-CC)

Technician:

Natasha Breaux (Texas A&M, Corpus Christi, TAMU-CC)

Data Manager:

Terry Palmer (Texas A&M, Corpus Christi, TAMU-CC)

BCO-DMO Data Manager:

Karen Soenen (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2023-02-24

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Salinity tolerance of oysters without acclimation in lab conditions: mortality

Abstract:

The mortality of oyster spat spawned from four different wild broodstocks (first filial generation) was measured when exposed to five different salinities (without acclimation) under controlled laboratory conditions. Oyster broodstocks were sourced from two populations in Louisiana (Calcasieu Lake; 29°50′58′′N, 93°17′1′′W, and Vermilion Bay; 29°34′47′′N, 92°2′4′′W) and two populations in Texas (Packery Channel; 27°37′38′′N, 97°13′59′′W, and Aransas Bay; 28°7′38′′N, 96°59′8′′W). Mortality was recorded in oyster spat that were exposed to salinities of 2, 4, 20, 38 and 44 without acclimation under laboratory conditions. Changes in water quality and spat size were also recorded.

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Archival Copy

Version Date	Archive	Persistent Identifier (PID)	Date PID Issued
2023-02-24	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.870210.1	2023-02-24

Description

A detailed description of methods was written by Marshall et al. (2021). In summary, mortality of oyster spat spawned from four different broodstocks was measured when exposed to five different salinities (without acclimation) under controlled laboratory conditions. Oyster broodstocks were sourced from two populations in Louisiana (Calcasieu Lake; 29°50′58′′N, 93°17′1′′W, and Vermilion Bay; 29°34′47′′N, 92°2′4′′W) and two populations in Texas (Packery Channel; 27°37′38′′N, 97°13′59′′W, and Aransas Bay; 28°7′38′′N, 96°59′8′′W). This salinity tolerance experiment was conducted in November–December 2018 (Trial 1) and March–April 2019 (Trial 2) at Texas A&M University-Corpus Christi.

Methods & Sampling

In each trial, 25 oysters from each stock were placed in labelled petri dishes within 15 38-L tanks (100 oysters per tank) with aerated artificial seawater (using Instant Ocean Reef Crystals Reef Salt, Blacksburg, Virginia) at 25 °C and salinities of 2.0, 4.0, 20.0, 38.0 and 44.0; each salinity treatment was replicated three times (5 salinity treatments × 3 replicate groups = 15 tanks). The temperature of each tank was maintained using Hydor 100W submersible glass aquarium heaters. Oxygen was supplied to each aquarium via air pump/stone, and tanks were heated to approx. 25 oC. Temperature and salinity from each tank were recorded daily. Oysters were fed daily with Shellfish Diet 1800® at approximately 3mL/100 oysters unless the water was cloudy (i.e., food from the previous day hadn’t been consumed). Trial 2 is a repeat of the same experiment conducted in Nov/Dec 2018 using the same oyster stock. The only difference is that three aquaria of salinity 20 were added with no airstone to compare oyster mortality in low and high oxygen concentrations.

Data Processing Description

Every other day, the numbers of live and dead oysters of each stock in each tank were counted over a 3-week period and the dead oysters were removed. Oysters were determined to be dead if their valves (shells) remained open despite any handling.

BCO-DMO processing:
* Merged tables of trial 1 and trial 2
* Added column Trial 1 and Trial 2 to be able to differentiate between the experiments.
* Added ISO_DateTime_UTC column based on date and time (in CDT) columns.

Data Files

File
mortality_data.csv (Comma Separated Values (.csv), 30.23 KB) MD5:0f62f2b4cb209e3dac5721ce97678289 Primary data file for dataset ID 870210

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1737207

Deployments

None available yet

Instruments

None available yet

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Trial	Experiment number: 1 or 2	unitless	no_bcodmo_term
Date	Date (Central Daylight Time)	unitless	date
Time	Time (Central Daylight Time)	unitless	time
ISO_DateTime_UTC	Sampling date and time in ISO format, UTC timezone Format: %Y-%m-%dT%H:%MZ	unitless	ISO_DateTime_UTC
Day	Day number of experiment	unitless	day
Tank	Tank, numbered 1-15	unitless	tank
Rep	Replicate, numbered 1-3	unitless	replicate
Treatment	Salinity Treatment	unitless	treatment
PC_mort	Packery Channel Population Mortalities	unitless	count
AB_mort	Aransas Bay Population Mortalities	unitless	count
VB_mort	Vermillion Bay Population Mortalities	unitless	count
CL_mort	Calcasieu Lake Population Mortalities	unitless	count
PC_cumort	Packery Channel Population Mortalities (Cumulative)	unitless	count
AB_cumort	Aransas Bay Population Mortalities (Cumulative)	unitless	count
VB_cumort	Vermillion Bay Population Mortalities (Cumulative)	unitless	count
CL_cumort	Calcasieu Lake Population Mortalities (Cumulative)	unitless	count
PC_live	Packery Channel Population Live Oysters (counted)	unitless	count
AB_live	Aransas Bay Population Live Oysters (counted)	unitless	count
VB_live	Vermillion Bay Population Live Oysters (counted)	unitless	count
CL_live	Calcasieu Lake Population Live Oysters (counted)	unitless	count

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Database

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Dataset: Salinity tolerance without acclimation: Mortality