Dataset: Salinity tolerance without acclimation: Water Quality

Name: Salinity tolerance of oysters without acclimation in lab conditions: Water Quality
Published: 2022-02-28
Keywords: oceans

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Cite This Dataset

Temporal Extent: 2018-11-29 - 2019-04-19

Project:

Collaborative Research: Testing for local adaptation and responses to multiple stressors in populations of eastern oysters inhabiting a natural salinity gradient (Oyster adaptation)

Principal Investigator:

Morgan Kelly (Louisiana State University, LSU)

Jennifer Beseres Pollack (Texas A&M, Corpus Christi, TAMU-CC)

Co-Principal Investigator:

Jerome La Peyre (Louisiana State University, LSU)

Contact:

Jennifer Beseres Pollack (Texas A&M, Corpus Christi, TAMU-CC)

Technician:

Natasha Breaux (Texas A&M, Corpus Christi, TAMU-CC)

Data Manager:

Terry Palmer (Texas A&M, Corpus Christi, TAMU-CC)

BCO-DMO Data Manager:

Karen Soenen (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2022-02-28

Restricted:

Validated:

Current State:

Preliminary and in progress

Salinity tolerance of oysters without acclimation in lab conditions: Water Quality

Abstract:

Salinity tolerance of oysters without acclimation in lab conditions: Water Quality

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Acquisition Description

A detailed description of methods was written by Marshall et al. (2021). In summary, mortality of oyster spat spawned from four different broodstocks was measured when exposed to five different salinities (without acclimation) under controlled laboratory conditions. Oyster broodstocks were sourced from two populations in Louisiana (Calcasieu Lake; 29°50′58′′N, 93°17′1′′W, and Vermilion Bay; 29°34′47′′N, 92°2′4′′W) and two populations in Texas (Packery Channel; 27°37′38′′N, 97°13′59′′W, and Aransas Bay; 28°7′38′′N, 96°59′8′′W). This salinity tolerance experiment was conducted in November–December 2018 (Trial 1) and March–April 2019 (Trial 2) at Texas A&M University-Corpus Christi.

In each trial, 25 oysters from each stock were placed in labelled petri dishes within 15 38-L tanks (100 oysters per tank) with aerated artificial seawater (using Instant Ocean Reef Crystals Reef Salt, Blacksburg, Virginia) at 25 °C and salinities of 2.0, 4.0, 20.0, 38.0 and 44.0; each salinity treatment was replicated three times (5 salinity treatments × 3 replicate groups = 15 tanks). The temperature of each tank was maintained using Hydor 100W submersible glass aquarium heaters. Temperature and salinity from each tank were recorded daily. Nutrient (NO2, NO2, NH3/NH4) concentrations in each tank were measured approximately weekly. Oysters were fed daily with Shellfish Diet 1800® unless the water was cloudy (i.e., food from the previous day hadn’t been consumed).

Processing Description

Every other day, the numbers of live and dead oysters of each stock in each tank were counted over a 3-week period and the dead oysters were removed. Oysters were determined to be dead if their valves (shells) remained open despite any handling. In trial two, oyster spat from each broodstock were held in three non-aerated aquaria of salinity 20 to compare mortality in low oxygen conditions.

Water temperature was measured using an aquarium thermometer or YSI ProDSS multiprobe. Salinity was measured using a Fisher Scientific portable refractometer or YSI ProDSS multiprobe. Dissolved oxygen concentrations and saturation, and pH were measured using a YSI ProDSS multiprobe. Nutrients were measured using API test strips or API Liquid Test Kit. YSI ProDSS sensors were calibrated at least weekly.

BCO-DMO processing:
* Added ISO_DateTime_UTC column based on date and time (in CDT) columns.
* Replaced , to ; in comments section to comply with database requirements
* Adjusted column names to comply with database requirements

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1737207

Deployments

None available yet

Instruments

Salinity Sensor

Supplied Name:

Supplied Description:

Fisher Scientific portable refractometer or YSI ProDSS multiprobe

Instrument Type

Generic Name: Salinity Sensor

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/350/

Generic Description:

Category of instrument that simultaneously measures electrical conductivity and temperature in the water column to provide temperature and salinity data.

Water Quality Multiprobe

Supplied Name: YSI ProDSS multiprobe

Supplied Description:

Water temperature was measured using an aquarium thermometer or YSI ProDSS multiprobe.Dissolved oxygen concentrations and saturation, and pH were measured using a YSI ProDSS multiprobe. YSI ProDSS sensors were calibrated at least weekly.

Instrument Type

Generic Name: Water Quality Multiprobe

Generic Description:

An instrument which measures multiple water quality parameters based on the sensor configuration.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Trial	Trial number, trial 1 or trial 2	unitless	exp_id
Date	Date in CDT timezone in ddMMMyyyy format Format: %d%b%Y	unitless	date
Time	Time in CDT timezone in hh:mm (24 hr) format Format: %H:%M	unitless	time
ISO_DateTime_UTC	Sampling date and time in UTC timezone in ISO format Format: %Y-%m-%dT%H:%MZ	unitless	ISO_DateTime_UTC
Tank	Tank	numbered 1-15	tank
Rep	Replicate	numbered 1-3	replicate
Treatment	Salinity Treatment (2, 4, 20, 38, 44)	unitless	treatment
Temp	Recorded Temperature (YSI ProDSS or aquarium thermometer)	Degrees Celsius (°C)	temp
Salinity	Recorded Salinity (Fisher portable refractometer or YSI ProDSS )	unitless	sal
NH3_NH4	Ammonia/Ammonium (API Test Strips/Liquid Test Kit)	ppm (mg/L)	unknown
NO2	Nitrite (Api Test Strips )	ppm (mg/L)	NO2
NO3	Nitrate (Api Test Strips )	ppm (mg/L)	NO3
pH	pH (YSI ProDSS)	unitless	pH
DO	Dissolved Oxygen (YSI ProDSS)	mg/L	O2
Source	Refractometer or YSI sonde, API strips or test kit	unitless	sample_descrip
Heater_Adjusted	Was heater adjusted? Y or N	unitless	sample_descrip
F_W_added	Volume of freshwater added	liters (L)	sample_descrip
Water_Change	Volume of water (of equal salinity) exchanged with tank	liters (L)	sample_descrip
Qty_Fed	Quantity of Reed 1800 Shellfish Diet provided (3mL Disposable Pippette)	mL	sample_descrip
Comments	Comments	unitless	comment

Database

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Dataset: Salinity tolerance without acclimation: Water Quality