Dataset: Water column production and respiration 2017

Name: Louisiana shelf water column production and respiration measured by change in oxygen in light and dark bottle incubations during four cruises in 2017
Published: 2023-05-24
Keywords: water column primary production, respiration, coastal hypoxia, coastal acidification, hurricanes, biota, oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.896385.1

Spatial Extent: N:29.6994 E:-88.5999 S:27.4998 W:-95.3041

Temporal Extent: 2017-04-06 - 2017-10-11

Project:

Collaborative Research: A RAPID response to Hurricane Harvey's impacts on coastal carbon cycle, metabolic balance and ocean acidification (HarveyCarbonCycle)

Principal Investigator:

John Lehrter (University of South Alabama; and Dauphin Island Sea Lab, USA-DISL)

Student:

Mai Fung (University of South Alabama; and Dauphin Island Sea Lab, USA-DISL)

Alexis Hagemeyer (University of South Alabama; and Dauphin Island Sea Lab, USA-DISL)

BCO-DMO Data Manager:

Dana Stuart Gerlach (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2023-05-24

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Louisiana shelf water column production and respiration measured by change in oxygen in light and dark bottle incubations during four cruises in 2017

Abstract:

These data include the measurements of oxygen, temperature, and salinity from incubations to measure rates of oxygen production in the light by primary production and oxygen consumption in the dark by community respiration. Data were collected during four cruises in 2017 during April, July, September and October. The September and October cruises followed the landfall of Hurricane Harvey. Cruises were conducted aboard the R/V Pelican in April (PE17-18), July (PE18-02), and October (PE18-11) and aboard the R/V Acadiana (AC18-12) in September. The study domain included the Louisiana and Texas continental shelf from the Mississippi River Bird's Foot delta to south of Galveston Bay. The objective of this work was to quantify how hurricanes and tropical storms affect metabolic rates and the concentrations of oxygen and DIC/pH. These data assess the water column response in metabolism before and after Hurricane Harvey and were collected by Dr. John Lehrter of the University of South Alabama.

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Archival Copy

Version Date	Archive	Persistent Identifier	Date Assigned
2023-05-24	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.896385.1	2023-07-21

Methods & Sampling

Samples for plankton community respiration (WR) measurements were collected from several depths in the water column including the surface layer, the bottom layer, the layer at depth of chlorophyll maximum, midwater oxygen maximum, and midwater oxygen minimum. For each sample collected, three transparent (light) glass bottles and three dark glass bottles were filled directly from the ship's Niskin bottle array (Murrell and Lehrter, 2011). Aliquots were put into acid-cleaned 300 milliliter BOD (biochemical oxygen demand) bottles for light-dark incubation experiments. After initial O₂ measurements were taken (T0), samples were placed in a darkened incubator that maintained surface water temperatures using water supplied continuously from the ship’s hull pump. Light bottle treatments were incubated in an outdoor incubator with screening that reduced ambient light by 50%. Dark bottle treatments were also placed in the outdoor incubator but were kept dark by their black liners and caps.

For more detailed methods see Murrell et al. (2013) and Murrell and Lehrter (2011).

Concentrations of O₂ were measured initially and after 24 hours using a Hach or YSI oxygen sensor and temperature probe. Salinity values for each sample were obtained from the ship's CTD (SeaBird 911). Net primary production rates were calculated as the change in O₂ over time in light bottles screened at 50% of ambient sunlight and incubated for 24 hours, expressed in units of millimoles oxygen per cubic meter per day (mmol O₂ m^-3 d^-1). Respiration rates were calculated as the change in O₂ over time in dark bottles incubated for 24 hours, expressed in units of millimoles oxygen per cubic meter per day (mmol O₂ m^-3 d^-1).

Problem report: Some of the incubation bottles had their caps come off during incubations. These values are labeled as NAN (not a number) in the dataset.

Note: Dissolved oxygen data were recorded in mg/L for the April 2017 cruise and in percent saturation for the July and September cruises.

Data Processing Description

Please see the Supplemental Files section for equations in more easily readable format, along with a brief summary of the method for light and dark bottle oxygen assay to measure primary production and respiration

Net primary production, respiration, and gross primary production calculations
Rates were adjusted to initial temperatures using the Arrhenius equation and initial and final temperatures.

Variables:

NPP is Net Primary Production
R is Respiration
GPP is Gross Primary Production
NPP_T is Temperature adjusted net primary production
R_T is Temperature adjusted respiration
GPP_T is Temperature adjusted gross primary production
DO_Light is the dissolved oxygen concentration in the light bottles
DO_Dark is the dissolved oxygen concentration in the dark bottles
TF = time final
T0 = time initial

Equations: (see Supplemental Files section)
NPP = (DO_Light_TF - DO_Light_T0)/(TF - T0)
R = (DO_Dark_TF - DO_Dark_T0)/(TF - T0)
GPP = NPP - R
NPP_T = NPP*2^((T_T0 - T_TF)./10)
R_T = R*2^((T_T0 - T_TF)./10)
GPP_T = GPP*2^((T_T0 - T_TF)./10)

Data Files

File
production_respiration.csv (Comma Separated Values (.csv), 78.03 KB) MD5:a755199ea3a38340282865ca676a8619 Production and respiration data from light and dark bottle incubations from Louisiana shelf water column

Supplemental Files

File
Rate_calculations_NPP_Resp_GPP.pdf (Portable Document Format (.pdf), 365.45 KB) MD5:5c20e22c354418d991976a73c6732425 Net primary production, respiration, and gross primary production methods and rate calculations

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1760747

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
AC18-12	AC18_12_Roberts GOM_Discrete_1709 GOM_Nutrients_1709	2017-09-17	R/V Acadiana	John Lehrter (Chief Scientist)	data info
PE17-18	GOM_UW_1704 GOM_CTD_1704	2017-04-05	R/V Pelican	Baoshan Chen (Chief Scientist)	data info
PE18-02	GOM_CTD_1707	2017-07-07	R/V Pelican	Wei-Jun Cai (Chief Scientist)	data info
PE18-11	GOM_CTD_1710 GOM_Discrete_1710 GOM_Discrete_1710	2017-09-28	R/V Pelican	Baoshan Chen (Chief Scientist)	data info

Instruments

CTD Sea-Bird 911

Supplied Name: CTD Seabird 911

Supplied Description:

Instrument Type

Generic Name: CTD Sea-Bird 911

Acronym: CTD SBE 911

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0035/

Generic Description:

The Sea-Bird SBE 911 is a type of CTD instrument package. The SBE 911 includes the SBE 9 Underwater Unit and the SBE 11 Deck Unit (for real-time readout using conductive wire) for deployment from a vessel. The combination of the SBE 9 and SBE 11 is called a SBE 911. The SBE 9 uses Sea-Bird's standard modular temperature and conductivity sensors (SBE 3 and SBE 4). The SBE 9 CTD can be configured with auxiliary sensors to measure other parameters including dissolved oxygen, pH, turbidity, fluorescence, light (PAR), light transmission, etc.). More information from Sea-Bird Electronics.

Niskin bottle

Supplied Description:

Samples were collected from several depths in the water column using Niskin bottles

Instrument Type

Generic Name: Niskin bottle

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/

Generic Description:

A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc.

Oxygen Sensor

Supplied Name: O2 probe with thermometer (Hach or YSI)

Supplied Description:

Concentrations of O2 were measured initially and after 24 hours using a Hach or YSI oxygen sensor and temperature probe.

Instrument Type

Generic Name: Oxygen Sensor

Acronym: Dissolved Oxygen Sensor

Generic Description:

An electronic device that measures the proportion of oxygen (O2) in the gas or liquid being analyzed

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Cruise	Cruise identification	unitless	cruise_id
Station	Station identification	unitless	station
Layer	Depth layer of water sample where S=surface, B=bottom, Chla max=midwater chlorophyll a maximum layer, O2 max=midwater oxygen maximum, or O2 min=midwater oxygen minimum	unitless	site_descrip
Latitude	Latitude of sample collection	decimal degrees	lat
Longitude	Longitude of sample collection	decimal degrees	lon
Sample_Collection_Date_Time	Date and time of sample collection in UTC Format: %Y-%m-%dT%H:%M:%S	unitless	ISO_DateTime_UTC
Depth_Bottom	Depth of the bottom	meters	depth_bottom
Bottle_Number	Sample bottle number from Niskin bottle array	unitless	bottle
Bottle_sampling_depth	Sampling depth of Niskin bottle	meters	depth
Salinity	Salinity from CTD sensor	psu	sal
DO_Light_1_T0	Initial dissolved oxygen concentration in Light bottle replicate 1	milligram per liter (mg/L) or percent saturation	O2
DO_Light_2_T0	Initial dissolved oxygen concentration in Light bottle replicate 2	milligram per liter (mg/L) or percent saturation	O2
DO_Light_3_T0	Initial dissolved oxygen concentration in Light bottle replicate 3	milligram per liter (mg/L) or percent saturation	O2
DO_Dark_1_T0	Initial dissolved oxygen concentration in Dark bottle replicate 1	milligram per liter (mg/L) or percent saturation	O2
DO_Dark_2_T0	Initial dissolved oxygen concentration in Dark bottle replicate 2	milligram per liter (mg/L) or percent saturation	O2
DO_Dark_3_T0	Initial dissolved oxygen concentration in Dark bottle replicate 3	milligram per liter (mg/L) or percent saturation	O2
T_Light_1_T0	Initial temperature of Light bottle replicate 1	degrees Celsius	temperature
T_Light_2_T0	Initial temperature of Light bottle replicate 2	degrees Celsius	temperature
T_Light_3_T0	Initial temperature of Light bottle replicate 3	degrees Celsius	temperature
T_Dark_1_T0	Initial temperature of Dark bottle replicate 1	degrees Celsius	temperature
T_Dark_2_T0	Initial temperature of Dark bottle replicate 2	degrees Celsius	temperature
T_Dark_3_T0	Initial temperature of Dark bottle replicate 3	degrees Celsius	temperature
DO_Light_1_TF	Final dissolved oxygen concentration in Light bottle replicate 1	milligram per liter (mg/L) or percent saturation	O2
DO_Light_2_TF	Final dissolved oxygen concentration in Light bottle replicate 2	milligram per liter (mg/L) or percent saturation	O2
DO_Light_3_TF	Final dissolved oxygen concentration in Light bottle replicate 3	milligram per liter (mg/L) or percent saturation	O2
DO_Dark_1_TF	Final dissolved oxygen concentration in Dark bottle replicate 1	milligram per liter (mg/L) or percent saturation	O2
DO_Dark_2_TF	Final dissolved oxygen concentration in Dark bottle replicate 2	milligram per liter (mg/L) or percent saturation	O2
DO_Dark_3_TF	Final dissolved oxygen concentration in Dark bottle replicate 3	milligram per liter (mg/L) or percent saturation	O2
T_Light_1_TF	Final temperature of Light bottle replicate 1	degrees Celsius	temperature
T_Light_2_TF	Final temperature of Light bottle replicate 2	degrees Celsius	temperature
T_Light_3_TF	Final temperature of Light bottle replicate 3	degrees Celsius	temperature
T_Dark_1_TF	Final temperature of Dark bottle replicate 1	degrees Celsius	temperature
T_Dark_2_TF	Final temperature of Dark bottle replicate 2	degrees Celsius	temperature
T_Dark_3_TF	Final temperature of Dark bottle replicate 3	degrees Celsius	temperature
CruiseStation	Concatenated Cruise and Station	unitless	site
Time_in_local	Incubation start time (local) when bottles were placed in the on-deck incubator Format: %Y-%m-%d %H:%M	unitless	DateTime
Time_out_local	Incubation end time (local) when bottles were taken out of the on-deck incubator Format: %Y-%m-%d %H:%M	unitless	DateTime

Database

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Dataset: Water column production and respiration 2017