Dataset: Tail wave amplitude in Oikopleura dioica at 3 temperature treatments

Name: Tail wave amplitude from 5 high-speed video frames at 3 temperatures from Oikopleura dioica particle tracking experiments conducted in December 2015
Published: 2023-06-22
Keywords: Oikopleura dioica, temperature, filter feeding, plankton, oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.897825.1

Temporal Extent: 2015-12-05 - 2015-12-10

Project:

Collaborative Research: Comparative feeding by gelatinous grazers on microbial prey (Gelatinous Grazer Feeding)

Principal Investigator:

Brad J. Gemmell (University of South Florida, USF)

Kelly Rakow Sutherland (University of Oregon)

Scientist:

Keats R. Conley (University of Oregon)

Terra C. Hiebert (University of Oregon)

George von Dassow (University of Oregon)

Contact:

Kelly Rakow Sutherland (University of Oregon)

BCO-DMO Data Manager:

Shannon Rauch (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2023-06-22

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Tail wave amplitude from 5 high-speed video frames at 3 temperatures from Oikopleura dioica particle tracking experiments conducted in December 2015

Abstract:

These data include tail beat kinematics measurements and particle tracking from the appendicularian Oikopleura dioica during experiments conducted in December 2015 at the Sars Centre for Marine Molecular Biology in Bergen, Norway. The data were collected from high-speed video frames. The experiments comprised 3 temperature treatments. This dataset includes measurements of tail wave amplitude. Tail beat kinematic measurements were used to describe how O. dioica drives flow across food concentrating filters and how it is affected by temperature.

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Archival Copy

Version Date	Archive	Persistent Identifier	Date Assigned
2023-06-22	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.897825.1	2023-06-30

Methods & Sampling

All experimental animals were obtained from the appendicularian culture facility at the Sars Centre for Marine Molecular Biology in Bergen, Norway in December 2015. Oikopleura dioica were filmed individually following Gemmell et al. (2014). Images were recorded using an Edgertronic high-speed camera (1280 × 1024-pixel resolution, 500 frames per second) with brightfield illumination from a fiber optic light source, or a Photron FastCam Mini Ux100 (1280x1024, 125-1000 frames per second) with darkfield illumination from a tilting mirror base. The filming vessel was positioned on a manually adjustable stage between the light source and the camera. A long working-distance microscope objective (4x or 40x) was mounted to an adjustable-height optics clamp positioned between the filming vessel and the camera. Videos were converted to image stacks in QuickTime Pro. Day 1 animals were filmed in a 50-milliliter glass cuvette in treatments comprising 3 temperatures: 5° Celsius, 15° Celsius, and 25° Celsius.

The videos are attached as Supplemental Files (there is one .zip folder for each temperature treatment).

Data Processing Description

Data Processing:
Tail beat kinematics were measured in ImageJ. Wave amplitude was determined from 5 frames per animal, with each frame taken at the maximum angle of the tail-trunk junction. Amplitude was measured as the maximum height from a line originating at the tail-trunk junction and bisecting the tail at the inflection point. Two amplitude measurements were taken for each frame.

BCO-DMO Processing:
- renamed fields to comply with BCO-DMO naming conventions;
- bundled the videos into 3 .zip files (see Supplemental Files).

Data Files

File
wave_amplitude.csv (Comma Separated Values (.csv), 13.61 KB) MD5:25539f9efd59106d8c4ea214b732ddd1 Primary data file for dataset ID 897825

Supplemental Files

File
15C.zip (ZIP Archive (ZIP), 1.94 GB) MD5:5ee628c77dd3b5079923bb2d4004a5dc This .zip contains 17 .mov video files from the 15 degrees Celsius treatments. See the file inventory (Appendicularian_Video_Inventory.csv) for a description of each file.
25C.zip (ZIP Archive (ZIP), 2.01 GB) MD5:02928e5f9fd97b1f5f73cd4dc8f5e6c4 This .zip contains 16 .mov video files from the 25 degrees Celsius treatments. See the file inventory (Appendicularian_Video_Inventory.csv) for a description of each file.
5C.zip (ZIP Archive (ZIP), 2.49 GB) MD5:6888cc317e68626484f97c5fd5a53df6 This .zip contains 20 .mov video files from the 5 degrees Celsius treatments. See the file inventory (Appendicularian_Video_Inventory.csv) for a description of each file.
Appendicularian_Video_Inventory.csv (Comma Separated Values (.csv), 2.77 KB) MD5:3984ad11c2e6a3e4373883742f6988ad Inventory/description of video files contained in the three .zip files - one for each temperature treatment. This inventory was originally provided as an Excel file and was converted to .csv by BCO-DMO.

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1851537

Deployments

None available yet

Instruments

high-speed camera

Supplied Name: Edgertronic high-speed camera

Supplied Description:

Instrument Type

Generic Name: high-speed camera

Generic Description:

A high-speed imaging camera is capable of recording rapid phenomena with high-frame rates. After recording, the images stored on the medium can be played back in slow motion. The functionality in a high-speed imaging device results from the frame rate, or the number of individual stills recorded in the period of one second (fps). Common video cameras will typically record about 24 to 40 fps, yet even low-end high-speed cameras will record 1,000 fps.

high-speed camera

Supplied Name: Photron FastCam Mini Ux100

Supplied Description:

Instrument Type

Generic Name: high-speed camera

Generic Description:

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Temperature	Temperature treatment	degrees Celsius	treatment
Video	Video identifier. The 10-digit video file names correspond to the .mov file names. The .mov files are provided in the attached .zip folders as Supplemental Files.	unitless	file_name
Frame	Video frame number	unitless	file_name
Time	Time at frame measured	seconds	duration
Wavelength	Sine wavelength	millimeters (mm)	wavelength
Tail_Length	Total tail length	millimeters (mm)	length
Amplitude_A	Maximum wave amplitude; two amplitude measurements were made per frame, and were denoted A and B.	millimeters (mm)	wave_stats
Amplitude_A_Norm	Maximum wave amplitude normalized to tail length	millimeters (mm)	wave_stats
Amplitude_B	Maximum wave amplitude; two amplitude measurements were made per frame, and were denoted A and B.	millimeters (mm)	wave_stats
Amplitude_B_norm	Maximum wave amplitude normalized to tail length	millimeters (mm)	wave_stats

Database

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Dataset: Tail wave amplitude in Oikopleura dioica at 3 temperature treatments