Dataset: P. tricornutum diel cycle

Name: Results of diel cycle experiments including the full range of volatile organic compounds (VOCs) produced by the diatom P. tricornutum during laboratory experiments conduced in October of 2023
Published: 2024-05-21
Keywords: Volatile organic compounds, Phaeodactylum tricornutum, diel cycle, VOC, oceans

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Laboratory study

Temporal Extent: 2023-10

Project:

Interactions between phytoplankton and bacterioplankton mediated by volatile organic compounds (Plankton Interactions and VOC)

Principal Investigator:

Kimberly Halsey (Oregon State University, OSU)

Student:

Hawra Almubarak (Oregon State University, OSU)

BCO-DMO Data Manager:

Amber D. York (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2024-05-21

Restricted:

Validated:

Current State:

Data not available

Results of diel cycle experiments including the full range of volatile organic compounds (VOCs) produced by the diatom P. tricornutum during laboratory experiments conduced in October of 2023

Abstract:

Algal photosynthetic metabolism changes depending on light availability. These metabolic shifts alter how photosynthetic energy is allocated into different biosynthetic and catabolic pathways yielding different metabolic intermediates and end products. Our research focuses on the metabolic intermediates and end products that are volatile organic compounds (VOCs). This dataset gives the full range of VOCs produced by the diatom, Phaeodactylum tricornutum, and detected by proton transfer reaction time of flight mass spectrometry over a 24 h day-night cycle. These diel cycle laboratory experiments were conducted in October of 2023. Light intensity was controlled by a LED system that was programmed to provide 12 h light over a sinusoidal period reaching a maximum intensity of 400 uE at noon. VOCs were collected every 2 hours.

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Methods & Sampling

Volatile organic compounds (VOCs) were collected from axenic Phaeodactylum tricornutum (urn:lsid:marinespecies.org:taxname:175584) every 2 hours across a diel cycle using proton transfer reaction time-of-flight mass spectroscopy (PTR-TOF/MS; Ionicon, Austria) as previously described (Moore et al., 2020). Autoclaved f/2+Si media and HPLC-grade water were measured alongside the samples as blank (background) controls. 100 ml culture, media, or HPLC water was transferred to a dynamic stripping chamber (Halsey et al., 2017)and bubbled with breathing-grade air passed first through a hydrocarbon trap and then through a glass frit in the bottom of the chamber at 50 ml min^-1 for 5 min to strip VOCs from the sample.

The stripped gasses were directed into the PTR-ToF/MS for measurement via soft ionization with H₃O⁺. The mass spectrum (30-240 a.m.u.) was acquired at 5 s intervals.

PTR-TOF/MS data were analyzed using PTR viewer 3.4.3 (Ionicon Analytik).

Media recipe:
Artificial seawater + 882 μM NO3 + 36.2 μM PO4 + 106 μM Si + 1x f/2 Trace metal mix + 1 x f/2 Vitamin mix

Data Processing Description

A three-point calibration was performed on each .h5 file using a search range of 0.2 m/z. Within the three-point calibration mode, each mass spectrum was calibrated against three internal standards known to be present within the spectrum, namely 29.998 (NO+), 203.943, and 330.848 (1,3-diiodobenzene).

The first 30 s of acquired data were removed from the analysis because of the instrument lag time between the chamber headspace and detector. PTR-TOF/MS data were analyzed using PTR viewer 3.4.3 (Ionicon Analytik).

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1948163

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Instruments

Mass Spectrometer

Supplied Name: Proton transfer reaction time-of-flight mass spectroscopy (PTR-TOF/MS; Ionicon, Austria)

Supplied Description:

Instrument Type

Generic Name: Mass Spectrometer

Acronym: Mass Spec

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/

Generic Description:

General term for instruments used to measure the mass-to-charge ratio of ions; generally used to find the composition of a sample by generating a mass spectrum representing the masses of sample components.

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Dataset: P. tricornutum diel cycle