Dataset: Sequence accession numbers for 16S rRNA genes characterizing microbiome changes in Agaricia tenuifolia and Siderastrea siderea coral species after being exposed to hypoxia and after a short period of recovery in 2022 experiments

We conducted in situ experiments in which oxygen was manipulated with benthic tent-like enclosures.  Experimental plots were 60 x 60 cm, spaced > 2 m apart, and were randomly assigned to one of three treatments: a) Fully closed tents made of 60 x 60 cm greenhouse-grade clear vinyl with a 60 cm flange extending outward from the edges. Water was circulated within tents with a submersible aquarium pump; b) Partially closed tents used as procedural controls consisting of tents with parts of two sides cut away and no flange; and c) Open plots with no tent. The treatment was imposed for two days followed by a recovery period. During the recovery, colonies were taken from the plots and placed nearby for an additional three days under ambient conditions.

Coral microbiome samples were collected a) 'before': just before the experiment, following one day of acclimatization, b) ‘during': after two days of exposure to the treatment of either fully closed tent, partially closed tent, or open plot, and c) ‘after’: three days after the end of the treatment period. 

For each sampled coral colony, 5 millimeter (mm) circular cores (consisting of tissue and skeleton) were collected by SCUBA divers using sterile disposable stainless-steel biopsy punches (Integra Miltex). On shore, coral fragments were preserved with RNAlater and frozen until processing at the University of Florida. DNA was extracted with a DNeasy PowerBiofilm kit (Qiagen). The V4 region of the 16S rRNA gene was amplified with the 515F and 806RB Earth Microbiome primers and thermocycler protocol.