©2026 Biological and Chemical Oceanography Data Management Office.Funded by the U.S. National Science Foundation
©2026 Biological and Chemical Oceanography Data Management Office.These data describe CTD-rosette water collections carried out during R/V Atlantis cruise AT50-08 in February 2023 in the Eastern Tropical North Pacific oxygen minimum zone (OMZ). Seawater was collected from Niskin bottles mounted on a CTD rosette at selected depths between ~50 and 600 m and was used as starting material for extracellular vesicle (EV) and viral particle purification workflows, as well as other microbial and biogeochemical measurements. These water-collection records provide the p...
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Seawater samples (10 × 100 L) were collected during R/V Atlantis cruise AT50-08 (Feb–Mar 2023) at two stations in the Oxygen Minimum Zone of the Eastern Tropical North Pacific (10–600 m depth). Samples were collected by CTD rosette, pre-filtered through 0.22 µm filters to remove cells and debris, and concentrated ~500× by tangential flow filtration (100 kDa cutoff).
The resulting concentrates contained both viruses and extracellular vesicles (EVs). EVs were enriched on board using a lectin-based binding column. Transmission electron microscopy (JEOL JEM 1400, Stony Brook University CryoEM facility) confirmed EV enrichment after column purification.
Subsequent EV analyses were performed at the University of Pennsylvania School of Veterinary Medicine EV Core Facility. Methods included size-based separation (SEC-HPLC, gravity-driven iZon column, density-gradient ultracentrifugation), particle quantification (resistive pulse sensing, nCS1; nanoparticle tracking analysis, NTA), and immunophenotyping (nanoscale flow cytometry; ExoView™ chip array). See related datasets.
Taylor, G. T., Yakubovskaya, E. (2026). CTD profile data from R/V Atlantis cruise AT50-08 in the ETNP oxygen minimum zone. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2026-03-24 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/995462 [access date]
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