Dataset: Thalassiosira Fe and light limitation - culture conditions
Deployment: Jenkins_2015

Culture conditions for T. oceanica and T. weissflogii grown in varied Fe and light
Principal Investigator: 
Bethany D. Jenkins (University of Rhode Island, URI)
Co-Principal Investigator: 
Phoebe Dreux Chappell (Old Dominion University, ODU)
BCO-DMO Data Manager: 
Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)
Current State: 
Final no updates expected
Version: 
2016-01-28
Version Date: 
2016-01-28
Description

Marine MicroEukaryote Transcriptome Sequencing Project (MMETSP) Sample Links:
iMicrobe accession: http://data.imicrobe.us/project/view/104
GenBank SRA accession: http://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA231566

Related Datasets:

Thalassiosira Fe and light limitation - ENA accessions
Thalassiosira Fe and light limitation - NCBI and iMicrobe accessions

References:

Media:
Guillard RRL, Hargraves PE (1993). Stichochrysis immobilis is a diatom, not a chyrsophyte. Phycologia 32: 234-236.

Culturing, RNA Extraction:
Chappell, P.D.*, L.P. Whitney*, J.R. Wallace, A.I. Darer, S. Jean-Charles, and B.D. Jenkins. 2015. Genetic indicators of iron limitation in wild populations of Thalassiosira oceanica from the northeast Pacific Ocean – ISME J. 9(3): 592-602. doi: 10.1038/ismej.2014.171 (* indicates co-first author)

Sequencing:
Keeling, P.J. et al. (2014) The Marine Microbial Eukaryote Transcriptome Sequencing Project (MMETSP): illuminating the functional diversity of eukaryotic life in the oceans through transcriptome sequencing. PLoS Biol, 12(6), e1001889. doi: 10.1371/journal.pbio.1001889

More information about this dataset deployment