Dataset: Acropora cervicornis photosynthesis and respiration rates
Deployment: Muller_Looe_Key_Reef_Acropora

Photosynthesis and Respiration rates of Acropora cervicornis

View Data: For data, See Dataset Metadata Page: https://www.bco-dmo.org/dataset/712366

Principal Investigator:

Erinn M. Muller (Mote Marine Laboratory, Mote)

BCO-DMO Data Manager:

Amber D. York (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

CAREER: Applying phenotypic variability to identify resilient Acropora cervicornis genotypes in the Florida Keys (Resilient Acerv)

Version:

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Description

This dataset contains oxygen change rates under dark and light conditions from 12 Acropora cervicornis genotypes previously exposed to different pH and temperature treatments. The coral surface area during photosynthesis and respiration experiments is included. The experiments were conducted in tanks at Summerland Key, Florida (24.6616,-81.4538) between 2016-09-02 and 2016-09-10 with corals from a nursery located near Looe Key Reef (24.5636, -81.2786).

Methods & Sampling

Dataset acquisition description

Physiological Methods

Photosynthesis, respiration, and calcification measurements were performed on each fragment using 300 mL temperature-controlled respirometry chambers filled with seawater from the treatment aquaria that was continuously stirred with a magnetic stir bar. The chambers were used to assess the rates of respiration (Rd) in the dark and rates of photosynthesis (Pn) and calcification in the light. Light was supplied by a series of blue and red LEDs with adjustable intensity (150 uMol quanta m-2 sec-1).

Data Processing Description

Dataset Processing Description

Photosynthesis and Respiration - The concentration of dissolved oxygen (O2, umol L-1) was continuously measured inside the chambers using optrodes (Oxy-4, Presense, Germany) to determine Rd (oxygen consumption) and Pn (oxygen production) in umol O2 cm-2 h-1. Rd rates were measured during incubation in complete darkness (60 minutes). Subsequently, light intensity was increased to 100 umol photons m-2 s-1 and Pn rates were measured (60 minutes). Rd and Pn were calculated as: Pn (or Rd) O2 = (sO2 x V)/S where sO2 is the slope of the linear regression line for change in O2 over time (umol L-1 h-1), V is the volume of the chamber (l) and S is the surface area of the fragment (cm2).

BCO-DMO Data Manager Processing Notes:
* added a conventional header with dataset name, PI name, version date
* modified parameter names to conform with BCO-DMO naming conventions

* renamed dark..umol.O2.h. light..umol.O2.h. to "O2_rate_dark" and "O2_rate_light" due to characer restrictions (no periods).

* rounded O2_rate dark and light to three decimal places
* "NA" and "na" changed to "nd" for "no data"

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-1452538	NSF Division of Ocean Sciences

Instruments

Optode

Supplied Name: optodes (Oxy-4, Presense, Germany)

Supplied Description:

Instrument Type

Generic Name: Optode

Generic Description:

An optode or optrode is an optical sensor device that optically measures a specific substance usually with the aid of a chemical transducer.

pH Sensor

Supplied Name: Mettler Toledo SevenGo Pro

Supplied Description:

Instrument Type

Generic Name: pH Sensor

Generic Description:

An instrument that measures the hydrogen ion activity in solutions.

The overall concentration of hydrogen ions is inversely related to its pH. The pH scale ranges from 0 to 14 and indicates whether acidic (more H+) or basic (less H+).

YSI Professional Plus Multi-Parameter Probe

Supplied Name: YSI Pro 2030

Supplied Description:

Temperature, salinity, and dissolved oxygen measured with YSI Pro 2030.

Instrument Type

Generic Name: YSI Professional Plus Multi-Parameter Probe

Acronym: YSI ProPlus

Generic Description:

The YSI Professional Plus handheld multiparameter meter provides for the measurement of a variety of combinations for dissolved oxygen, conductivity, specific conductance, salinity, resistivity, total dissolved solids (TDS), pH, ORP, pH/ORP combination, ammonium (ammonia), nitrate, chloride and temperature. More information from the manufacturer.

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
date	Day the measurement took place in format yyyy-mm-dd	unitless	date
O2_rate_dark	Rate of change (slope) in oxygen levels as coral sat in the dark for 60 minutes	micromoles of oxygen per hour (umol/h)	O2_respir
O2_rate_light	Rate of change (slope) in oxygen levels as coral sat in light conditions for 60 minutes	micromoles of oxygen per hour (umol/h)	O2_respir
genotype	Genotype number of the coral animal for each fragment	unitless	sample_descrip
tank	Tank number that held the particular coral fragment	unitless	tank
temp	Treatment temperature level	Celsius	temp
pH	Treatment pH level; ambient = 8.1 pH; hCO2 = 7.7 pH	unitless	pH
area	Surface area of the coral	centimeters squared (cm2)	surface_area

Database

Contribute Data

Dataset: Acropora cervicornis photosynthesis and respiration rates
Deployment: Muller_Looe_Key_Reef_Acropora

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: Acropora cervicornis photosynthesis and respiration ratesDeployment: Muller_Looe_Key_Reef_Acropora

Dataset acquisition description

Dataset Processing Description

Dataset: Acropora cervicornis photosynthesis and respiration rates
Deployment: Muller_Looe_Key_Reef_Acropora