Dataset: AR16 Experiment Chl a

Name: Chlorophyll a concentrations from addition incubation experiments performed on AR16 from May 2017 in the Sargasso Sea
Published: 2019-08-21
Keywords: oceans

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Temporal Extent: 2017-05-10 - 2017-05-20

Project:

Collaborative Research: Defining the biogeochemical drivers of diatom physiological ecology in the North Atlantic (North Atlantic Diatoms)

Principal Investigator:

Tatiana Rynearson (University of Rhode Island, URI-GSO)

Co-Principal Investigator:

Sonya T. Dyhrman (Columbia University)

Bethany D. Jenkins (University of Rhode Island, URI-GSO)

BCO-DMO Data Manager:

Mathew Biddle (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2019-08-21

Restricted:

Validated:

Current State:

Data not available yet

Chlorophyll a concentrations from addition incubation experiments performed on AR16 from May 2017 in the Sargasso Sea

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Description

Chlorophyll a concentrations from addition incubation experiments performed on AR16.

Acquisition Description

In situ Chlorophyll a was measured at the conclusion of each incubation experiment conducted in triplicate on AR16. Samples were taken from biological replicates and filtered onto GF/F and 5µm polycarbonate filters.

Chl a was extracted from filters in 100% ethanol for 12 hours, measured on a 10-AU Fluorometer, and chlorophyll a and phaeophytin concentrations were calculated based on a predetermined calibration curve. Concentrations on each filter were used to determine total chl a within each size fraction.

Processing Description

Data was processed in R 3.4.1 (R-Core-Team 2017).

BCO-DMO Processing Notes:
- added conventional header with dataset name, PI name, version date
- modified parameter names to conform with BCO-DMO naming conventions
- removed greater than symbol (>) from size_fraction field.
- reformatted date from mm/dd/yy HH:MM to yyyy-mm-ddTHH:MM:SS to conform with the ISO 8601 convention

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1558490

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
AR16	PHAT – Phosphorus, Hydrocarbons, and Trichodesmium	2017-05-03	R/V Neil Armstrong	Benjamin A.S. Van Mooy (Chief Scientist)	data info

Instruments

Turner Designs Fluorometer 10-AU

(more info)

Short Name: Turner Fluorometer 10-AU

Community Standard Description

Generic Description

The Turner Designs 10-AU Field Fluorometer is used to measure Chlorophyll fluorescence. The 10AU Fluorometer can be set up for continuous-flow monitoring or discrete sample analyses. A variety of compounds can be measured using application-specific optical filters available from the manufacturer. (read more from Turner Designs, turnerdesigns.com, Sunnyvale, CA, USA)

PI supplied instrument name: 10AU Fluorometer

Dataset-specific description

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
datetime	Date and time of incubation conclusion in UTC following ISO 8601 format	unitless	ISO_DateTime_UTC
experiment	Experiment number in series	unitless	exp_id
treatment	Experimental treatment	unitless	treatment
bottle_number	Incubation bottle that was sampled	unitless	bottle
replicate	Biological replicate of triplicate incubations	unitless	replicate
size_fraction	greater than size fraction analyzed based on pore size of filter	micrometers (um)	unknown
vol_filtered	Volume of seawater filtered for chl a analysis	milliliters (ml)	vol_filt
vol_extracted	Volume of ethanol used to extract chla a	milliliters (ml)	volume
Fb	Fluorescence before acidification	RFU	fluor
Fa	Fluroescence after acidification	RFU	fluor
Notes	Notes regarding sample	unitless	comment
blank_subt_Fb	Blank corrected fluorescence before acidification	RFU	fluor
blank_subt_Fa	Blank corrected fluorescence after acidification	RFU	fluor
chla	Chlorophyll a concentration	micrograms per liter (ug/L)	chl_a
phaeo	Phaeophytin concentration	micrograms per liter (ug/L)	phaeo

Database

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Dataset: AR16 Experiment Chl a