Dataset: Heatwave Experiment: Photosynthetic Irradiance

Name: Photosynthetic irradiance capacity of coral fragments measured during a heatwave experiment done September to November 2018 using reef building corals collected in Kāne'ohe Bay, O'ahu, Hawai'i
Published: 2023-01-31
Keywords: oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.884537.1

Temporal Extent: 2018-09-22 - 2018-11-17

Project:

NSFOCE-BSF: COLLABORATIVE RESEARCH: Elucidating adaptive potential through coral holobiont functional integration (Holobiont Integration)

Co-Principal Investigator:

Hollie Putnam (University of Rhode Island, URI)

Student:

Emma Strand (University of Rhode Island, URI)

BCO-DMO Data Manager:

Karen Soenen

Version:

Version Date:

2023-01-31

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Photosynthetic irradiance capacity of coral fragments measured during a heatwave experiment done September to November 2018 using reef building corals collected in Kāne'ohe Bay, O'ahu, Hawai'i

Abstract:

Two common reef-building corals, Montipora capitata and Pocillopora acuta, were collected from six sites in Kāne'ohe Bay, O'ahu, Hawai'i. Fragments were allowed to acclimate in experimental tanks for two weeks prior to exposure to one of the following four treatments: Ambient Temperature Ambient pCO2 (ATAC), Ambient Temperature High pCO2 (ATHC), High Temperature Ambient pCO2 (HTAC), and High Temperature High pCO2 (HTHC). The treatment period lasted for a two month period, starting on September 22nd, 2018 and lasting through November 17th, 2018. Following the stress period, coral fragments were exposed to a two-month recovery period in ambient conditions. Fragments were measured for photosynthetic irradiance capacity at four time points during the stress period. This dataset includes calculated outputs from those measurements.

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Archival Copy

Version Date	Archive	Persistent Identifier	Date Assigned
2023-01-31	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.884537.1	2023-01-31

Methods & Sampling

Photosynthetic Irradiance Curves: Prior to experimental exposures, 10 coral fragments (5 per species) were used to generate a photosynthesis-irradiance curve to determine saturating irradiance for assessing rates of photosynthesis. Fragments were exposed to 10 light levels: 0, 15, 30, 60, 91, 136, 227, 416, 529, and 756 µmol m-2 s-1 generated by two LED lights (Aqua Illumination Hydra FiftyTwo) hung above the incubation chambers (described below). Rates of oxygen consumption or evolution were extracted using curve fitting of a non-linear least squares fit for a non-rectangular hyperbola (NLLS; Marshall & Biscoe 1980, Heberling 2013) was used to identify PI curve characteristics of each species. This model is as follows: = фPAR+√(φPPFD + Pmax)2-4ΘφPAR Pmax2Θ-Rd . Theta was set at 0.6 for M. capitata and 0.64 for P. acuta. Photosynthesis-irradiance curves were performed four times throughout the experiment on fragments of both species in the HTHC treatment to determine that there were no significant changes in Ik that occured during bleaching, and thus no need to change light settings used to measure respiration and photosynthetic rates.

Fragments were placed in individual respiration chambers (~610mL), with individual temperature (Pt1000 temperature sensor, PreSens) and fiber-optic oxygen probes (Oxygen Dipping Probes DP-PSt7, accuracy = ± 0.05% O2, PreSens) connected to a 10-channel oxygen meter (OXY-10 ST, accuracy = ± 1.0 °C, resolution = 0.1 °C, PreSens), to evaluate photosynthesis under saturating light conditions, as determined by PI curves described above, and light enhanced dark respiration (LEDR; Edmunds and Davies 1988). The respirometry setup consisted of 10 chambers with stirbars. Samples were measured in a series of runs that consisted of eight fragments (n=4 per species and n=2 blank chambers) per run, and exposed to PAR irradiance of 590 ± 7.16 µmol photons m-2 s-1 for 15 minutes to assess photosynthetic rates. Immediately afterwards, these fragments were exposed to dark conditions (0 µmol photons m-2 s-1) for 20 minutes to assess LEDR. Following respirometry, fragments were set back in their respective tanks.

Data Processing Description

Raw photosynthetic (temperature corrected) data was thinned every 40 seconds and rates of oxygen flux were calculated by local linear regressions in LoLinR package (Olito et al. 2017) in µmol L-1 s-1. Rates were corrected to chamber volume including coral displacement. Rates of the blank chambers were subtracted from the rates of the coral fragments within each run to account for background photosynthesis or respiration due to phytoplankton, zooplankton, and bacteria. Rates of oxygen flux were normalized to surface area by the wax dipping technique (Veal et al. 2010) for final units of µmol cm-2 h-1. Net photosynthesis was calculated by subtracting the respiration rates of the dark runs from the gross photosynthetic rates of the light runs, to isolate photosynthetic rates of the symbionts from the light enhanced dark respiration (LEDR) of the coral holobiont.

Data Files

File
heatwave_irradiance_all.csv (Comma Separated Values (.csv), 94.77 KB) MD5:338e4ce06ce1489ca89f1f76f9b7a6cc Primary data file for dataset ID 884537
PI_Curves filename: PI_curve_rates.zip (ZIP Archive (ZIP), 30.12 KB) MD5:7e285fae8d99020c59d3966891d1ad32 Individual .csv files of photosynthetic irradiance curves for dataset 884537

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1756623

Deployments

None available yet

Instruments

Biospherical PAR sensor

Supplied Name: Apex cosine corrected PAR Sensor

Supplied Description:

Apex cosine corrected PAR Sensor to measure light

Instrument Type

Generic Name: Biospherical PAR sensor

Generic Description:

Unspecified Biospherical PAR. An irradiance sensor, designed to measure Photosynthetically Active Radiation (PAR).

LI-COR LI-192 PAR Sensor

Supplied Name:

Supplied Description:

Li-Cor cosine corrected PAR sensor (LI-192) to measure light

Instrument Type

Generic Name: LI-COR LI-192 PAR Sensor

Acronym: LI-COR LI-192 PAR

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0120/

Generic Description:

The LI-192 Underwater Quantum Sensor (UWQ) measures underwater or atmospheric Photon Flux Density (PPFD) (Photosynthetically Available Radiation from 360 degrees) using a Silicon Photodiode and glass filters encased in a waterproof housing. The LI-192 is cosine corrected and features corrosion resistant, rugged construction for use in freshwater or saltwater and pressures up to 800 psi (5500 kPa, 560 meters depth). Typical output is in um s-1 m-2. The LI-192 uses computer-tailored filter glass to achieve the desired quantum response. Calibration is traceable to NIST. The LI-192 serial numbers begin with UWQ-XXXXX. LI-COR has been producing Underwater Quantum Sensors since 1973.

These LI-192 sensors are typically listed as LI-192SA to designate the 2-pin connector on the base of the housing and require an Underwater Cable (LI-COR part number 2222UWB) to connect to the pins on the Sensor and connect to a data recording device.

The LI-192 differs from the LI-193 primarily in sensitivity and angular response.
193: Sensitivity: Typically 7 uA per 1000 umol s-1 m-2 in water. Azimuth: < ± 3% error over 360° at 90° from normal axis. Angular Response: < ± 4% error up to ± 90° from normal axis.
192: Sensitivity: Typically 4 uA per 1000 umol s-1 m-2 in water. Azimuth: < ± 1% error over 360° at 45° elevation. Cosine Correction: Optimized for underwater and atmospheric use.
(www.licor.com)

Oxygen Microelectrode Sensor

Supplied Name: Fiber-optic oxygen probes Oxygen Dipping Probes DP-PSt7 PreSens

Supplied Description:

Instrument Type

Generic Name: Oxygen Microelectrode Sensor

Acronym: O2 Microelectrode

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/351/

Generic Description:

Any microelectrode sensor that measures oxygen.

PreSens OXY-10 Mini oxygen meter

Supplied Name:

Supplied Description:

Instrument Type

Generic Name: PreSens OXY-10 Mini oxygen meter

Generic Description:

The OXY-10 mini is a precise multi-channel oxygen meter for up to 10 'in-house' sensors, simultaneously controlling and reading them. The meter is used with oxygen sensors based on a 2mm optical fibre. The meter is compatible with sensors that are type PSt3 which has a detection limit 15 ppb, 0 - 100% oxygen.

Water Temperature Sensor

Supplied Name: Pt1000 temperature sensor, PreSens

Supplied Description:

Instrument Type

Generic Name: Water Temperature Sensor

Acronym: Water Temp Sensor

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/134/

Generic Description:

General term for an instrument that measures the temperature of the water with which it is in contact (thermometer).

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
sampling_point	Record number	unitless	number
Light_Level	Light level number on scale of 1-10	unitless	sample_descrip
Fragment_ID	Fragment replicate ID number with species initials (PA, MC) indicated	unitless	sample
Intercept	Intercept value from PreSens	umol/L/second (micromol per liter per second)	sample_descrip
Aumol_L_sec	change in dissolved oxygen value	umol per liter per second	O2
Temp	Temperature at that time of umol.L.sec data point	degrees Celsius (°C)	temp
Date	Date of PI curve	units	date
Sample_ID	Fragment.ID and Date	unitless	sample
Plug_Number	The individual coral fragment ID number	unitless	sample
Chamber_Channel	The chamber and channel ID number that was associated with that coral fragment during PI curve	unitless	sample_descrip
Position	Position in the PI curve set up associated with that chamber during that run	unitless	sample_descrip
Bin_ID	The bin in the PI curve set up associated with that chamber during that run	unitless	sample_descrip
Run	PI curve run number for that sampling date	unitless	sample_descrip
Species	Coral host species: Montipora capitata or Pocillopora acuta	unitless	species
Fragment_Number	Fragment replicate ID number	unitless	sample
Sample_Type	Sample or Blank indication	unitless	sample_descrip
Temp_Cat	Temperature of calibration from PreSens	degrees Celsius (°C)	temp
Chamber_Vol_L	Volume in liters of the chamber that coral fragment was in	liters (L)	volume
Surf_Area_cm2	Estimated surface area of that coral fragment	cm2	surface_area
Start_time	Time that PI curve light level was started	unitless	time_start
Stop_Time	Time that PI curve light level ended	unitless	time_end
Light_Dark	Light or Dark indication	unitless	sample_descrip
Light_Value	PAR value for that light level	nm (nanometers)	PAR
Notes	Notes taken during PI curve in the field	unitless	comment
QC	Notes taken during quality control	unitless	comment
sample_micromol_s	Calculated coral fragment's PI curve rate for that light level	umol per liter per second	irradiance
blank_micromol_s	The calculated blank chamber's PI curve rate for that light level	umol per liter per second	irradiance
corr_micromol_s	Calculated corrected value (sample value - blank value; sample.micromol.s - blank.micromol.s)	umol per liter per second	irradiance
micromol_cm2_s	Calculated corrected value normalized to surface area (corr.micromol.s/Surf.Area.cm2)	umol per liter per second	irradiance
micromol_cm2_h	Calculated corrected value	umol per liter per hour	irradiance
source_file	The name of the source ifle	unitless	file_name

Database

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Dataset: Heatwave Experiment: Photosynthetic Irradiance