Dataset: Short-term heat stress assay: Photochemical yield data

Name: Photochemical yield and bleaching severity data from a standardized short-term (18 hour) heat stress assay of Montipora capitata and Porites compressa collected at a patch reef in Kane'ohe Bay, O'ahu, Hawai'i in September of 2022
Published: 2023-11-09
Keywords: climate change, ocean warming, environmental memory, oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.914723.1

Spatial Extent: N:21.4509 E:-157.7954 S:21.4509 W:-157.7954

Temporal Extent: 2022-09-01 - 2022-09-05

Project:

RAPID: Collaborative Research: Disentangling the effects of heat stress versus bleaching phenotype on coral performance (Mcap pairs time series)

Principal Investigator:

Katie Barott (University of Pennsylvania, Penn)

Hollie Putnam (University of Rhode Island, URI)

Co-Principal Investigator:

Kristen Brown (University of Pennsylvania, Penn)

BCO-DMO Data Manager:

Amber D. York (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2023-11-09

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Photochemical yield and bleaching severity data from a standardized short-term (18 hour) heat stress assay of Montipora capitata and Porites compressa collected at a patch reef in Kane'ohe Bay, O'ahu, Hawai'i in September of 2022

Abstract:

Increasingly frequent marine heatwaves are devastating coral reefs. Corals that survive these extreme events must rapidly recover if they are to withstand subsequent events, and long-term survival in the face of rising ocean temperatures may hinge on recovery capacity and acclimatory gains in heat tolerance over an individual's lifespan. To better understand coral recovery trajectories in the face of successive marine heatwaves, we monitored the responses of bleaching-susceptible and bleaching-resistant individuals of two dominant coral species in Hawaiʻi, Montipora capitata and Porites compressa, over a decade that included three marine heatwaves. This dataset includes the photochemical yield from the standardized short-term (18 hour) heat stress assay.

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Archival Copy

Version Date	Archive	Persistent Identifier	Date Assigned
2023-11-09	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.914723.1	2023-11-28

Description

This dataset and other data from this study will be published in the results paper "Divergent bleaching and recovery trajectories in reef-building corals following a decade of successive marine heatwaves." (see pre-print Brown, et al. (2023), doi: 10.1101/2023.07.16.549193).

All BCO-DMO datasets related to this publication can be found on the page https://www.bco-dmo.org/related-resource/915300.

See Related Datasets section for "Short-term heat stress assay: Temperature data" from the same short-term (18 hour) heat stress assay.

Methods & Sampling

Sampling Location: patch reef 13 in Kāne'ohe Bay, O'ahu, Hawai'i (21.4509, -157.7954)

The following methods describe how this dataset was produced along with the related dataset "Short-term heat stress assay: Temperature data".

The following methods describe how this dataset was produced along with the related dataset "Short-term heat stress assay: temperature data" (https://www.bco-dmo.org/dataset/914715)

Acute heat stress experiment

Ten individual colonies of each phenotype per species were sampled on September 1, 2022. Four fragments were collected from each individual colony (i.e., genetic clones) by hand using bone cutters, totalling 160 fragments. Following collection, corals were transported to the Hawaiʻi Institute of Marine Biology (HIMB) and placed in outdoor, flow-through seawater tanks under ambient temperatures (mean ± SD; 28.2 ± 0.1°C, n=2 tanks) until experimentation. Coral thermal tolerance experiments were initiated 72 hours after collection. The experimental heat stress assay followed the standardized temperature profile used to measure heat tolerance in corals (e.g., MMM, MMM+3°C, MMM+6°C, and MMM+9°C) (Voolstra, et al., 2020). The experiment began on September 4 2022 at 13:00 with a 3-hour ramp to respective treatment temperatures (27.5°C, 30.5°C, 33.5°C, 36.5°C), a 3-hour hold, and a 1-hour ramp down to MMM temperature (27.5°C). A fragment from each coral colony was suspended using fishing line and randomly placed into each treatment, so that all genotypes across species were present in each treatment. Temperatures were controlled using a custom computer controlled system (Raspberry Pi). Temperatures were constantly monitored and manipulated by responding to probe measurements in real time, and recorded using cross-calibrated temperature loggers (accuracy: ± 0.47°C at 25°C; resolution: ± 0.10°C at 25°C HOBO UA-001-64, Onset Computer Corporation). Experiments were performed outdoors under natural photosynthetically active radiation (PAR), which ranged between 515–580 µmol m-2 sec-1 at midday (~12:00; Licor cosine sensor). At the end of the ramp and 1 h after sunset (~19:30), corals were assessed for dark-adapted photochemical yield (Fv/Fm) using a Diving-PAM (Walz GmbH) 5-mm diameter fiber-optic probe at a standardized distance (5 mm) above the coral tissue after F0 stabilized. Three random spots (2–3 cm apart across the front and back of the nubbin) were measured on each fragment to obtain average measures of Fv/Fm. All readings with F0 values that were less than 110 were removed to avoid any false detections (Marzonie et al., 2022). The following morning at 07:00 corals were photographed with a color standard (WDKK Waterproof Color Chart, DGK Color Tools) to assess coral color, a proxy for bleaching severity. Bleaching severity was determined visually (e.g., 0, 20, 40, 60, 80, 100% white) (Evensen, et al.., 2022) using the six gray standards as a reference (e.g., black= 0% bleached, white = 100% bleached) from two photographs (front and back of the fragment) to obtain an average color score (Brown et al., 2023 Fig. S4).

Taxonomic Identifiers:

Montipora capitata, LSID (urn:lsid:marinespecies.org:taxname:287697)

Porites compressa, LSID (urn:lsid:marinespecies.org:taxname:207236)

Data Processing Description

See results publication Brown, et al. (2023) for more detailed information on analysis and results.

BCO-DMO Processing

* Table within file "PAM data for STHS.csv " was imported into the BCO-DMO data system with values "NA" as missing data values.
** Missing data values are displayed differently based on the file format you download. They are blank in csv files, "NaN" in MatLab files, etc.

* Column names adjusted to conform to BCO-DMO naming conventions designed to support broad re-use by a variety of research tools and scripting languages. [Only numbers, letters, and underscores. Can not start with a number]

Data Files

File
914723_v1_sths-pam-data.csv (Comma Separated Values (.csv), 26.17 KB) MD5:5a28a43b133fd301e4a0d1e5c71cfba9 Primary data file for dataset ID 914723, version 1

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-2102989
NSF Division of Ocean Sciences	OCE-2103067

Deployments

None available yet

Instruments

Fluorometer

Supplied Name: Diving-PAM (Walz GmbH)

Supplied Description:

Diving-PAM (Walz GmbH) is an Underwater Fluorometer with Miniature Spectrometer.
Pulse-amplitude modulated (PAM) fluorometry.
Photochemical yield (Fv/Fm).

Instrument Type

Generic Name: Fluorometer

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/113/

Generic Description:

A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ.

Spectrometer

Supplied Name:

Supplied Description:

Diving-PAM (Walz GmbH) is an Underwater Fluorometer with Miniature Spectrometer.

Instrument Type

Generic Name: Spectrometer

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0460/

Generic Description:

A spectrometer is an optical instrument used to measure properties of light over a specific portion of the electromagnetic spectrum.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Treatment	Treatment identifier (AMB=ambient; or 3C, 6C, 9C)	unitless	treatment
Temperature	Temperature	degrees Celcius	temperature
Species	Species (Genus species)	unitless	species
Bleach	Bleach (phenotype)	unitless	sample_descrip
Colony_ID	Colony identifier	unitless	sample
Rep	replicate	unitless	replicate
F	minimum fluorescence value	unitless	no_bcodmo_term
Y	photochemical yield	unitless	no_bcodmo_term
M	maximum amount of fluorescence	unitless	no_bcodmo_term

Database

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Dataset: Short-term heat stress assay: Photochemical yield data