This dataset contains Flow cytometry on samples collected from Orcas Island, WA, USA Coastal Ocean (2m depth) every four hours from 5/28/21 to 6/18/21 collected as part of the following study.
Study abstract
Floating, single-celled algae, or phytoplankton, form the base of marine food webs. When phytoplankton have sufficient nutrients to grow quickly and generate dense populations, known as blooms, they influence productivity of the entire food web, including rich coastal fisheries. Th...
Centroid: latitude: 48.6765, longitude: -122.883233 Temporal Extent: 2021-05-28 - 2021-06-18 Coastal waters, East Sound, WA, USA, depth 2 m
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Data File(s)
Type
Description
Action
984014_v1_flow-cytometry.csv (20.45 KB)
Comma Separated Values (.csv)
Primary data file for dataset ID 984014, version 1
Additional funding description:
This dataset was supported by NSF OCE-2401646, OCE-2401645, OCE-2401644, University of Washington Royalty Research Fund, NIH NIEHS grant R21ES034337-01, NSF IOS-2041497, NIH fellowship F31 ES032733-01A1
The Figshare holding contains different (pre-curation) versions of several BCO-DMO datasets. See BCO-DMO dataset pages for notes about additional curation changes that resulted in slight differences between the Figshare and BCO-DMO-published data.
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L. Nunn, B., Timmins-Schiffman, E., Mudge, M. C., Plubell, D. L., Chebli, G., Kubanek, J., Riffle, M., Noble, W. S., Harvey, E., Nunn, T., Huntemann, M., Clum, A., Foster, B., Foster, B., Roux, S., Palaniappan, K., Mukherjee, S., Reddy, T. B. K., Daum, C., … Eloe-Fadrosh, E. A. (2024). Microbial Metagenomes across a Full Phytoplankton Bloom: High-Resolution Sampling Every 4 Hours for 22 Days [Data set]. figshare. https://doi.org/10.6084/M9.FIGSHARE.26882737
Repository containing all raw data and metadata files relating to the two papers published using the data from this project Nunn et al (2024, doi: 10.1038/s41597-024-04013-5) and Mudge et al. (2025, doi: 10.1038/s41467-025-59250-y).
Nunn, B, & Timmins-Schiffman, E. (2025). Nunn-Lab/Publication-2021-Orcas-Island-Time-Series: Nat.Comm Harmful algal blooms are preceded by a predictable and quantifiable shift in the oceanic microbiome (Orcas_metaproteomics). Zenodo. https://doi.org/10.5281/zenodo.14976385
These data were used in results publications from this project Nunn et al (2024, doi: 10.1038/s41597-024-04013-5) and Mudge et al. (2025, doi: 10.1038/s41467-025-59250-y).
Nunn, B. L., Kubanek, J., Rynearson, T. A., Timmins-Schiffman, E., Mudge, M., Bartlett, E. (2025) Environmental YSI EXO1 Sonde Probe data from Orcas Island, WA, USA Coastal Ocean (2m depth) from 2021-05-27 to 2021-06-18. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-09-10 doi:10.26008/1912/bco-dmo.984153.1
These data were used in results publications from this project Nunn et al (2024, doi: 10.1038/s41597-024-04013-5) and Mudge et al. (2025, doi: 10.1038/s41467-025-59250-y).
Nunn, B. L., Kubanek, J., Rynearson, T. A., Timmins-Schiffman, E., Mudge, M., Bartlett, E. (2025) Metagenomic sample information, genetic accession identifiers (NCBI SRA, JGI IMG), and estimated gene copies from Orcas Island coastal waters (2 m depth) from 2021-05-27 to 2021-06-18. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-09-10 doi:10.26008/1912/bco-dmo.984169.1
These data were used in results publications from this project Nunn et al (2024, doi: 10.1038/s41597-024-04013-5) and Mudge et al. (2025, doi: 10.1038/s41467-025-59250-y).
Nunn, B. L., Kubanek, J., Rynearson, T. A., Timmins-Schiffman, E., Mudge, M., Bartlett, E. (2025) Nutrient data for samples collected every 4 hours from Orcas Island, WA, USA Coastal Ocean (2m depth) during the period from 2021-05-27 to 2021-06-18. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-09-09 doi:10.26008/1912/bco-dmo.984065.1
Related Publications
Results
Mudge, M. C., Riffle, M., Chebli, G., Plubell, D. L., Rynearson, T. A., Noble, W. S., Timmins-Schiffman, E., Kubanek, J., & Nunn, B. L. (2025). Harmful algal blooms are preceded by a predictable and quantifiable shift in the oceanic microbiome. Nature Communications, 16(1). https://doi.org/10.1038/s41467-025-59250-y
Results
Nunn, B. L., Timmins-Schiffman, E., Mudge, M. C., Plubell, D. L., Chebli, G., Kubanek, J., Riffle, M., Noble, W. S., Harvey, E., Nunn, T. A., Rynearson, T., Huntemann, M., LaButti, K., Foster, B., Roux, S., Palaniappan, K., Mukherjee, S., Reddy, T. B. K., … Eloe-Fadrosh, E. A. (2024). Microbial Metagenomes Across a Complete Phytoplankton Bloom Cycle: High-Resolution Sampling Every 4 Hours Over 22 Days. Scientific Data, 11(1). https://doi.org/10.1038/s41597-024-04013-5
Nunn, B. L., Kubanek, J., Rynearson, T. A., Timmins-Schiffman, E., Mudge, M., Bartlett, E. (2025). Flow cytometry data from samples collected from Orcas Island, WA, USA Coastal Ocean (2m depth) every four hours from 2021-05-28 to 2021-06-18. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2025-09-09 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.984014.1 [access date]
If you wish to use this dataset, it is highly recommended that you contact the original principal investigators (PI). Should the relevant PI be unavailable, please contact BCO-DMO (info@bco-dmo.org) for additional guidance. For general guidance please see the BCO-DMO Terms of Use document.