| All 7Be data pertaining to upwelling velocity calculations from R/V Yellowfin cruises to the San Pedro Ocean Time-series (SPOT) in 2013 and 2014 | | centrifuge |
| Acropora cervicornis protein measurements: nutrient- and disease-exposed from samples collected at Mote Marine Laboratory in situ nursery from June to July 2022 | | Eppendorf centrifuge 5810R |
| Water column phosphate data from RV/Atlantic Explorer AE1812, May 2018 | Used to collect precipitate for soluble reactive phosphorus (SRP) measurements.
| |
| Microbial enzyme activities: peptidase activities of sediment samples from the RV\Polarstern cruise ARKXXVII/3 in the Central Arctic Ocean and Laptev Sea, Aug-Sept. 2012 | | |
| Porewater geochemistry, including methane, CO2, sulfate, and sulfide from Guaymas Basin hydrothermal sediments collected on R/V Atlantis cruise AT15-56 in Nov-Dec 2009 | | centrifuge |
| ATP and cell numbers in laboratory experiments with Thalassiosira weissflogii conducted in 2018 | | |
| ATP and cell numbers in laboratory experiments with Thalassiosira weissflogii conducted in 2019 | | |
| Porewater geochemistry of sediments collected Fall 2019 in the Santa Barbara Basin using ROV Jason during R/V Atlantis cruise AT42-19 | Porewater was separated from sediment by centrifugation (4300 x g for 20 mins) in anoxic centrifugation vials and subsampled for further analysis.
| centrifuge |
| Survey biogeochemical data from R/V Atlantis AT32, AT34, AT38, and AT39-06 in the western North Atlantic Ocean (35°N to 57°N; 45°W) in Nov. 2015, May 2016, Sep 2017, Mar/Apr 2018 | For measurement of bacterial production.
| |
| Hepatopancreas lipid content of crabs fed different diets collected at the Baruch Institute in 2014 (Variation in Metabolic Processes project) | Centrifuge Damon/IEC Division IEC HN-SII
| |
| Discrete bottle samples collected during BATS Validation (BVAL) cruises from April 1991 through July 2024 | All tubes are centrifuged at 14,000 RPM and 4°C for 7 minutes
| centrifuge |
| Pore water data from sediment cores collected from R/V Oceanus cruise OC1906A and R/V Sikuliaq cruise SKQ202016S off the coast of California in 2019 and 2020 | | Centrifuge |
| Biogeochemical data on cryptic methane cycling in hypersaline sediments of the Carpinteria Salt Marsh Reserve, California | | centrifugation |
| Coccolithophore-associated organic biopolymers for fractionating particle-reactive radionuclides (234Th, 233Pa, 210Pb, 210Po, and 7Be) | Beckman Coulter Allegra X-12 centrifuge
| Beckman Coulter Allegra X-12 centrifuge |
| Accession numbers and images of coral samples taken in the Florida Keys during 2013 (Coral Microbial Relationships project) | Tissue homogenate was then vortexed for 2 min and centrifuged at 20,000 x g for 20 min at 4 deg C
| Centrifuge |
| Coral fragment surface area calculations utilizing two methods (tin foil and Image J) and corresponding zooxanthellae count data | | |
| Symbiodinium cultures isolated from the octocoral Antillogorgia bipinnata in the Florida Keys and processed at Coffroth lab at the University at Buffalo in 2008, 2013 and 2016 (Host Symbiont Temp Response project) | This slurry was spun for 5 min at 500-800 rpm on a Beckman J6-HC centrifuge to pellet symbiont cells, the supernatant removed and responded in 10 ml of FSW.
| Beckman J6-HC centrifuge |
| Ambient concentrations of acetate, glycerol, and mannitol measured from samples collected during R/V Endeavor cruise EN616 in the northwest Atlantic in July 2018 | Following transfer to centrifuge tubes and centrifugation for 5min at 8000rcf, 1.4mL of the supernatant was removed
| centrifuge |
| Transcriptome and viral metagenome data from Diadema antillarum collected at St. Thomas (USVI) and Saba (Caribbean Netherlands) in Apr 2022 | The homogenates were centrifuged at 3,000 × g for 1 min to remove large cell debris, before filtering the supernatant through 0.2 µm pore size polyethersulfone syringe filters.
| Centrifuge |
| Bacterial protein production on particles obtained by gravity filtration of water collected on RV/Endeavor EN556 (Patterns of activities project) | Used to concentrate cell material.
| |
| Large volume incubation: DOC, bacterial cell concentration and production from RV/Endeavor EN556, June 2015 (Patterns of activities project) | Used to concentrate cell material.
| |
| Measurements of nutrients, bacterial cell concentration and bacterial production from RV/Endeavor EN556, June 2015 (Patterns of activities project) | Used to concentrate cell material.
| |
| Bacterial Productivity measurement of bulk seawater and mesocosm experiments taken aboard the R/V Endeavor in the Western North Atlantic during the research cruise EN683 in May and June, 2022 | Live samples were killed with 100% (w/v) TCA and centrifuged (10,000 rpm at 4°C for 10 min) to pelletize cell material.
| Centrifuged |
| Monosaccharide Composition of HMWDOM concentrate and POM-derived Carbohydrates from bulk seawater and mesocosm experiments taken aboard the R/V Endeavor in the Western North Atlantic during the research cruise EN683 in May and June, 2022 | Subsequently solvents were added to the filter pieces, vortexed and incubated for 2 h at 60°C at 650 rpm in a heat block. Extracts were centrifuged at 6,000 x g for 15 min at 15°C and the supernatant was transferred to a new tube.
| Centrifuge |
| Estimated frequency of lytic viral infection from samples collected in the Eastern Tropical North Pacific oxygen minimum zone region (ETNP OMZ) on R/V New Horizon cruise NH1315 during June 2013 | Air-driven ultracentrifuge (Airfuge CLS, Beckman Coulter, Brea, CA, USA)
| Air-driven ultracentrifuge (Beckman) |
| Morphotypes, capsid widths, and tail lengths of viruses from samples collected in the Eastern Tropical North Pacific oxygen minimum zone region (ETNP OMZ) on R/V New Horizon cruise NH1315 during June 2013 | Air-driven ultracentrifuge (Airfuge CLS, Beckman Coulter, Brea, CA, USA)
| Air-driven ultracentrifuge (Beckman) |
| NCBI accession metadata for Eukaryotic viruses encoding ribosomal protein eL40 from samples collected on KM1419 and KM1108 from Mar 2011 to Sep 2014 | Viruses in the filtrate were concentrated by TFF (30 kDa) to 300 mL volume, further concentrated to 0.5 mL by centrifugal ultrafiltration (30 kDa) and then purified in a CsCl buoyant density gradient.
| centrifugal ultrafiltration |
| FASTA file of protein identifications from R/V Kilo Moana KM1128 in the North Pacific during 2011 (MetZyme project) | | 5 kD membrane centrifuge |
| Partitioning of iron and plutonium in exopolymeric substances and intracellular biopolymers: a comparison study between the coccolithophore Emiliania huxleyi and the diatom Skeletonema costatum | Beckman Coulter Allegra X-12 centrifuge
| Beckman Coulter Allegra X-12 centrifuge |
| Iron concentrations of phage from experiments of iron-labelled E. coli infected with T4 and T5 bacteriophage, 2018 and 2019. | Used to concentrate cells and separate bacteria from phage.
| Beckman Coulter SW40Ti swinging bucket ultracentrifuge |
| Dissolved Th/Pa measurements from CTD Niskin collected depth profiles from a GEOTRACES transect cruise along the Mid-Atlantic Ridge in the North Atlantic (GA13/JC156) | | centrifuge |
| Microbial gene abundance of coastal wetland soil cores collected in June 2018 from Barataria Bay, Louisiana | | |
| Gene expression of Pocillopora damicornis collected from reef of Heron Island, southern Great Barrier Reef from Jan 2021 to Feb 2021 | Following homogenization, 400 μl of homogenate was removed and centrifuged for 3 mins at 9000 rcf.
| Centrifuge |
| Geochemistry and microscopy from incubation experiments of Chesapeake Bay sediments conducted at Horn Point Laboratory | | |
| Concentrations of dissolved thorium and protactinium isotopes (232Th, 230Th, 231Pa) in seawater collected during the Indian GEOTRACES Transect (SK312; GI02) in April-May 2014 | | IEC CL30, Thermo Scientific |
| Concentrations of dissolved thorium and protactinium isotopes (232Th, 230Th, 231Pa) in seawater collected during the Indian GEOTRACES Transect (SK324; GI05) in September-October 2015 | | IEC CL30, Thermo Scientific |
| Concentrations of dissolved thorium and protactinium isotopes (Th-232, Th-230, Pa-231) in seawater, sea ice, and melt ponds collected during the U.S. GEOTRACES Arctic cruise (HLY1502, GN01) on USCGC Healy from August to October 2015 | | Centrifuge |
| Concentrations of size-fractionated particulate thorium and protactinium isotopes (Th-232, Th-230, Pa-231) in seawater collected during the U.S. GEOTRACES Arctic cruise (HLY1502, GN01) on USCGC Healy from August to October 2015 | | Centrifuge |
| Dissolved thorium and protactinium isotopes (232Th, 230Th, 231Pa) in seawater from Leg 1 (Seattle, WA to Hilo, HI) of the US GEOTRACES Pacific Meridional Transect (PMT) cruise (GP15, RR1814) on R/V Roger Revelle from September to October 2018 | | centrifuge tubes |
| Dissolved thorium and protactinium isotopes (232Th, 230Th, 231Pa) in seawater from Leg 2 (Hilo, HI to Papeete, French Polynesia) of the US GEOTRACES Pacific Meridional Transect (PMT) cruise (GP15, RR1815) on R/V Roger Revelle from Oct-Nov 2018 | | centrifuge tubes |
| Depth profiles of seawater dissolved 232Th, 230Th, and 231Pa from R/V Sonne cruise SO245 (UltraPac, GPpr09) during Dec 2015 to Jan 2016 | | |
| Depth profiles of seawater particulate 232Th, 230Th, and 231Pa from R/V Sonne cruise SO245 (UltraPac, GPpr09) during Dec 2015 to Jan 2016 | | |
| Data pertaining to the development of larvae past the planula stage from experiments investigating heat priming in Nematostella vectensis | | centrifuge |
| Data pertaining to dose-response curves (DRC) quantifying survival of larvae after exposure to heat ramps from experiments investigating heat priming in Nematostella vectensis | | centrifuge |
| Expression of heat shock protein 70 (HSP70) in larvae at 11 days post-fertilization (DPF) from experiments investigating heat priming in Nematostella vectensis | | centrifuge |
| Heat tolerance (survival) of juveniles at 6 WPF following heat shock from experiments investigating heat priming in Nematostella vectensis | | centrifuge |
| Lethal temperature 50s (LT50s) displayed by larvae derived from dose-response curves after heat shock from experiments investigating heat priming in Nematostella vectensis | | centrifuge |
| Long-term body column lengths and tentacle numbers of larvae and juveniles from experiments investigating heat priming in Nematostella vectensis | | centrifuge |
| Respiration rates and protein content of larvae from experiments investigating heat priming in Nematostella vectensis | | centrifuge |
| Sizes of larvae from 4-11 days post-fertilization from experiments investigating heat priming in Nematostella vectensis | | centrifuge |
| Coral physiology parameters acquired during a heatwave experiment done September to November 2018 using reef building corals collected in Kāne'ohe Bay, O'ahu, Hawai'i. | | |
| Viral metagenomic investigation of the Caribbean echinoderm Holothuria floridana collected in Marathon, Florida on 25 Aug 2023 | The homogenates were centrifuged at 3,000 × g for 1 min to remove large cell debris, before filtering the supernatant through 0.2 µm pore size polyethersulfone syringe filters.
| Centrifuge |
| Metaproteomics analyses of mixed-layer water from Hawaii Ocean Time-series Station ALOHA, collected on R/V Kilo Moana cruise KM2204 in March 2022, and incubated with 15N2 to track biosynthetic incorporation by diazotrophic microbes | | Eppendorf 5430, 5424 |
| Analysis protocols, sample information, code, and datasets associated with the manuscript Structure and long-term stability of the microbiome in diverse diatom cultures from samples collected in Guam, California, and Gulf of Mexico between 2008 and 2016 | | Eppendorf 5414 C microcentrifuge |
| Mesozooplankton grazing rates from samples collected in the oceanic Gulf of Mexico on R/V Nancy Foster cruises NF1704 and NF1802 in May 2017 and May 2018 | The homogenate was shaken and centrifuged (5 min at 3000 rpm) to remove particulates.
| centrifuge |
| Metal quotas (ratios of Metal:P) of the polar diatom Chaetoceros sp. RS19 in +Zn and +Co incubation studies from January 2020 (MM Saito project) | Biomass from replicate 25 mL double addition cultures of Chaetoceros RS19 were pooled upon entering stationary phase and were centrifuged at 11,000 RPM (14,610 x g) for 40 minutes at 4°C. The cell pellet was resuspended in ~1 mL media and transferred to an acid-cleaned microcentrifuge tube. Cultures were centrifuged again for 30 min at 14,100 RPM (13,336 x g) at 4°C before the supernatant was discarded. Half of the remaining cell pellet was acidified in 800 mL of 5% nitric acid (Optima) containing 1 ppb indium for at least seven days while the other half was retained for proteomic analysis. Solids were removed by centrifugation.
| Centrifuge |
| Acropora hyacinthus isotope data from coral samples collected at a north shore site in Mo’orea in May 2019 | The blastate was then homogenized and centrifuged (2,000 x g for 2 min) to separate coral host tissue from endosymbiont cells. After supernatant (coral host) removal, the endosymbiont pellet was then resuspended in 2 mL of FSW and centrifuged an additional time to separate out any remaining coral host tissue. The supernatant from this centrifugation was combined with the coral host fraction. The coral host fraction was centrifuged an additional time to pellet any endosymbiont cells remaining in solution.
| Centrifuge |
| Carbonate chemistry from Mud Patch porewater, from R/V Endeavor EN524 in the continental shelf off New England; 40.43 N 70.5 W from May 2013 (OA, Hypoxia and Warming project) | Used to extract porewater from core
| centrifuge |
| Depth profiles of seawater dissolved 232Th, 230Th, and 231Pa from RVIB Nathaniel B. Palmer cruise NBP1702 from January to March 2017 | | |
| Global proteome analyses of the nitrite-oxidizing bacterium Nitrospira marina grown under atmospheric and low oxygen concentrations | | MWCO Vivaspin units (Sartorius Stedim, Goettingen, Germany) |
| Targeted proteome analyses of the nitrite-oxidizing bacterium Nitrospira marina grown under atmospheric and low oxygen concentrations | | MWCO Vivaspin units (Sartorius Stedim, Goettingen, Germany) |
| Depth profiles of pore water constituents from sediment cores collected on the Louisiana Shelf of the Northern Gulf of Mexico during 2020, 2021, and 2022 on R/V Savannah cruises SAV-20-07, SAV-21-25, SAV-21-24, SAV-22-06, and SAV-22-11 | Aldrich two-glove atmospheric bags were used to control the atmosphere during pore water extraction and centrifugation with an ELMI CM-7S clinical centrifuge.
| ELMI CM-7S centrifuge |
| Data on how nutrient and sediment loading affect coral functionality in a tropical branching coral | | Fisher Scientific accuSpin 3R |
| Nutrient properties of coastal wetland soil cores collected in June 2018 from Barataria Bay, Louisiana | | |
| Standing stocks and rates for organic sulfur compounds during summer in the subarctic Northeast Pacific | | |
| Activities of extracellular peptidases at Station H of the White Oak River (WOR) Station H in 2013 (SEDpep project) | | centrifuge |
| Peptides and their spectral counts from KM1128 the METZYME expedition on R/V Kilo Moana in the tropical North Pacific in 2011 | Used to concentrate the extracts
| 5 kD membrane centrifuge |
| Peroxide quantification in Thalassia testudinum tissue and response to pathogenic Labyrinthula in seagrass collected February 2024 in Tampa Bay, Florida | Samples were centrifuged at 17,000 x g for 10 min
| Centrifuge |
| Dynamics of the Apostichopus californicus-associated flavivirus under suboxic conditions and organic matter amendment in mesocosm experiment | Following this, tubes were centrifuged at 16,000 x g at 4°C for 20 min, and supernatant removed into a new microcentrifuge tube.
| Centrifuge |
| Physiological responses of the coral host and associated endosymbionts measured during two experiments testing the effects of acute and chronic thermal stress on the scleractinian coral, Stylophora pistillata | | |
| Analysis of temporal dynamics of three virus types persistently co-infecting Pleurochrysis carterae CCMP645 from laboratory experiments at the Bigelow Laboratory for Ocean Sciences, Maine from 2015-2016 | | |
| Metabolite and microbiota data from sea urchin specimens and seawater samples collected in 2023 from four locations in coastal waters of Puerto Rico | The Eppendorf 5810 R (Eppendorf AG, Hamburg, Germany; purchased 2012) is a high-capacity refrigerated benchtop centrifuge used for sample clarification before GC–MS analysis. It supports swing-bucket and fixed-angle rotors up to 400 mL and reaches 14,000 rpm (20,913 × g). The temperature range (−9 °C to +40 °C) and microprocessor-controlled cooling system ensure reproducible processing of temperature-sensitive metabolomic samples.
| Eppendorf 5810 R Refrigerated Centrifuge |
| Experimental observations of radionuclide uptake by colloidal and particulate humic acids obtained from 14 soils collected worldwide | Beckman Coulter Allegra X-12 centrifuge
| Beckman Coulter Allegra X-12 centrifuge |
| Sequence read accession (SRA) numbers and collection metadata for coral microbiome collected in Moorea, French Polynesia from Jul 2018 to Aug 2020 | | Eppendorf Benchtop Centrifuge 5430 |
| Sanger Sequencing Analysis of Potexvirus PCR Products (Potex-5 and Potex-2RC) collected during West Florida Coastal Surveys of Seagrass from Feb 2022 to Oct 2023 | Tissue homogenates were centrifuged at maximum speed (21,130 g) for 1 minute and RNA was extracted from the total volume (~800 μL) of the supernatant.
| Centrifuge |
| Application of a rapid microbiome characterization pipeline to corals afflicted with Stony Coral Tissue Loss Disease in St. Thomas, US Virgin Islands. | Used to separate tissue, mucus, and microbes from seawater
| centrifuge |
| Site descriptions and physical environmental conditions in coral microbiomes in the Florida Keys during 2013 (Coral Microbial Relationships project) | Tissue homogenate was then vortexed for 2 min and centrifuged at 20,000 x g for 20 min at 4 deg C
| Centrifuge |
| Geochemical composition of sediment pore water samples collected in the Equatorial Pacific during October and November 2020 on R/V Kilo Moana cruise KM2012 | | centrifuge |
| Sediment core porewater and particulate measurements from three sites on the Louisiana Shelf sampled during R/V Pelican cruises in August 2021 | Mulitcorer samples were placed into tubes and centrifuged at 3500 rpm for 10 minutes to extract and filter supernatant, which was later used for dissolved silica analysis.
| centrifuge |
| Sediment core porewater and particulate measurements from three sites on the Louisiana Shelf sampled during R/V Pelican cruises from December 2021 through August 2022 | Mulitcorer samples were placed into tubes and centrifuged at 3500 rpm for 10 minutes to extract and filter supernatant, which was later used for dissolved silica analysis.
| centrifuge |
| Soil physicochemical properties of coastal wetland soil cores collected in June 2018 from Barataria Bay, Louisiana | | |
| ssDNA-dsDNA viromes from the Great Lakes, off Bermuda and the South Pacific (GOV project) | Used to concentrate viral particles
| Amicon Ultra 100 kDa centrifugal devices (Millipore) |
| Sulfate triple oxygen isotope signatures in Messinian evaporite and barite from cores collected | Samples were then centrifuged, rinsed 3 times with DI, and dried in a 60 °C oven.
| Centrifuged |
| Fluorescence spectra for 3 strains of Synechococcus while increasing temperatures to detect the photosystem components disassociation temperature | Used to create a pellet of cells.
| centrifuge |
| Temperature data measured during two experiments testing the effects of acute and chronic thermal stress on the scleractinian coral, Stylophora pistillata | | |
| Infectivity assays and ultrastructural characterization of Thalassia testudinum agroinfected with Turtle grass virus X (TGVX) in laboratory aquaria, February-May 2024 | Microcentrifuge: Eppendorf 5424 – used for pelleting and separation steps in nucleic acid extraction.
| Eppendorf 5424 |
| Metabolomic data collected during a thermal stress experiment using reef building corals collected in Kāne'ohe Bay, O'ahu, Hawai'i. | | |
| Untargeted metabolomic data collected during a thermal stress experiment using reef building corals collected in Kāne'ohe Bay, O'ahu, Hawai'i. | | |
| Bioproject accession information on tag-sequence data for Pisaster ochraceus samples collected from Bodega Bay, CA, in July 2019 | Following centrifugation at 11.8 rcf for 15 minutes at 4°C, the upper clear, aqueous layer was carefully transferred to a new tube. RNA was precipitated with the addition of 500 µl isopropyl alcohol, then incubated for 10 minutes at RT and centrifuged at 11.8 rcf for 10 minutes at 4°C.
| Centrifuge |
| Comparative analysis of tissue biomass and energy reserves of six coral species from nearshore and offshore reefs in Palau, Micronesia during March 2017 | After centrifugation for 10 minutes at 1000g, 1 mL of the supernatant containing total carbohydrates was collected.
| IEC clinical centrifuge |
| Total spectral count of proteins from R/V Kilo Moana cruise KM1128 for the METZYME expedition in the tropical North Pacific in 2011. | Used to concentrate the extracts
| 5 kD membrane centrifuge |
| Nutrient transfer experiments with host coral and symbionts under varying environmental conditions conducted March 2014 and March 2015 | Symbiotic dinoflagellates and coral tissue were separated by 2–3 centrifugation washes (550 g for 5 min) using IEC clinical centrifuge (Damon/IEC Division, Needham Heights., MA)
| IEC clinical centrifuge |
| COI mtDNA sequences for trematodes from fish collections across the Northern Line Islands and French Polynesia archipelagos collected between 2009 and 2023 | Eppendorf tabletop centrifuge
| Eppendorf tabletop centrifuge |
| Water column analytical data from the Chile Triple Junction collected on two cruises aboard the R/V Melville during February-March 2010 and April 2012 | | centrifuge |
©2020 Biological and Chemical Oceanography Data Management Office.