Dataset: Heatwave Experiment: Color Score Physiology

Name: Physiology color score extracted from pictures taken during a heatwave experiment done September to November 2018 using reef building corals collected in Kāne'ohe Bay, O'ahu, Hawai'i.
Published: 2022-11-23
Keywords: oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.884208.1

Spatial Extent: N:21.477194 E:-157.786861 S:21.429417 W:-157.833667

Temporal Extent: 2018-09-22 - 2018-11-17

Project:

NSFOCE-BSF: COLLABORATIVE RESEARCH: Elucidating adaptive potential through coral holobiont functional integration (Holobiont Integration)

Co-Principal Investigator:

Hollie Putnam (University of Rhode Island, URI)

Student:

Emma Strand (University of Rhode Island, URI)

BCO-DMO Data Manager:

Karen Soenen (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2022-11-23

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Physiology color score extracted from pictures taken during a heatwave experiment done September to November 2018 using reef building corals collected in Kāne'ohe Bay, O'ahu, Hawai'i.

Abstract:

Two common reef-building corals, Montipora capitata and Pocillopora acuta, were subjected to a extended heatwave scenario. Fragments were allowed to acclimate in experimental tanks for two weeks prior to exposure to one of the following four treatments: Ambient Temperature Ambient pCO2 (ATAC), Ambient Temperature High pCO2 (ATHC), High Temperature Ambient pCO2 (HTAC), and High Temperature High pCO2 (HTHC). The treatment period lasted for a two month period, starting on September 22nd, 2018 and lasting through November 17th, 2018. Following the stress period, coral fragments were exposed to a two-month recovery period in ambient conditions. Throughout the entire four-month experiment, photos of the coral individuals were taken to extract a "color score". This dataset contains the processed data to calculate "color score" values.

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Archival Copy

Version Date	Archive	Persistent Identifier	Date Assigned
2022-11-23	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.884208.1	2023-02-07

Methods & Sampling

Corals sampled at six reefs within Kāne'ohe Bay, O'ahu, Hawai'i:

1.) USA: Hawaii HIMB: 21.436056, -157.786861
2.) USA: Hawaii Reef.11.13: 21.450806, -157.794944
3.) USA: Hawaii Reef.35.36: 21.473889, -157.833667
4.) USA: Hawaii Reef.18: 21.450806, -157.811139
5.) USA: Hawaii Lilipuna.Fringe: 21.429417, -157.791111
6.) USA: Hawaii Reef.42.43: 21.477194, -157.826889

Experiment conducted at the Hawai'i Institute of Marine Biology.

To assess tissue color change over time, each fragment was photographed once a week (16 timepoints in 4 month experimental setup) with a red, blue, green color standard ruler.

Data Processing Description

ImageJ® (version 1.51) was used to extract the mean blue, green, and red color score for each coral fragment and these values were normalized to the mean of the red, blue, and green color standards. The color score was quantified as Principal Component 1 from principal component analysis (PCA) sensu (Edmunds, Gates and Gleason, 2003).

BCO-DMO Processing Notes:
* Added latitude and longitude of reef locations to the dataset

Data Files

File
heatwave_colorscore.csv (Comma Separated Values (.csv), 373.61 KB) MD5:b332370404806dff43249e9ae62b81eb Primary data file for dataset ID 884208

Supplemental Files

File
Heatwave_Color_Score_Images.zip filename: Heatwave_Color_Score_Images_Emma_Strand.zip (ZIP Archive (ZIP), 1.27 GB) MD5:0d52fe0ccdaecffe5edf35971aacba51 Pictures of coral during a heatwave experiment where physiology color score has been extracted from using ImageJ.

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1756623

Deployments

None available yet

Instruments

ruler

Supplied Name: Ruler with three red, blue, and green colored bands

Supplied Description:

Ruler with three red, blue, and green colored bands

Instrument Type

Generic Name: ruler

Generic Description:

A device used for measuring or for drawing straight lines, consisting of an elongated piece of rigid or semi-rigid material marked with units for measurement. Device that allows one or more physical dimensions of a sample or specimen to be determined by visible comparison against marked graduations in units of measurement of dimension length.

Underwater Camera

Supplied Name:

Supplied Description:

Instrument Type

Generic Name: Underwater Camera

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/180/

Generic Description:

All types of photographic equipment that may be deployed underwater including stills, video, film and digital systems.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Number	Record number	unitless	number
Site_Name	The reef location where that sample was collected (6 options total).	unitless	site
Site_Latitude	Latitude of coral sampling site, south is negative	decimal degrees	lat
Site_Longitude	Longitude of coral sampling site, west is negative	decimal degrees	lon
Plug_ID	The individual coral fragment ID number	unitless	sample
Species	Coral host species: Montipora capitata or Pocillopora acuta	unitless	species
ANALYSIS	Physiology for all entries indicating that fragment was sampled for physiology	unitless	exp_type
Timepoint	The sampling time point; at what point during the experiment was that fragment sampled	unitless	time_sample
Treatment	Temperature and pCO2 treatment exposure indication. Ambient Temperature Ambient pCO2 (ATAC), Ambient Temperature High pCO2 (ATHC), High Temperature Ambient pCO2 (HTAC) or High Temperature High pCO2 (HTHC).	unitless	treatment
Tank	The tank number that coral fragment was sampled from (12 options; 3 tanks per treatment).	unitless	tank
Temperature	Temperature treatment indication: high or ambient.	unitless	treatment
CO2	pCO2 treatment indication: high or ambient.	unitless	treatment
Bleaching_Score	Calculated PC1 value from Principal Components Analysis used as a "Bleaching Score" or "Color Score"	unitless	sample_descrip
Blch_Time	Time point for this measurement.	unitless	time_sample

Database

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Dataset: Heatwave Experiment: Color Score Physiology