Dataset: BATS Validation pigments
View Data: Data not available yet
Data Citation:
Bates, N., Johnson, R. J., Medley, C., Lethaby, P. J. (2024) HPLC and fluorometric derived phytoplankton pigment data from BATS Validation cruises for years 1996-2023. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 4) Version Date 2024-05-03 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/926534 [access date]
Terms of Use
This dataset is licensed under Creative Commons Attribution 4.0.
If you wish to use this dataset, it is highly recommended that you contact the original principal investigators (PI). Should the relevant PI be unavailable, please contact BCO-DMO (info@bco-dmo.org) for additional guidance. For general guidance please see the BCO-DMO Terms of Use document.
Spatial Extent: N:36 E:-60 S:19 W:-80
Survey cruises in the Sargasso Sea ranging from 19N to 36N and 60W to 80W
Temporal Extent: 1996-06 - 2023-06
Project:
Program:
U.S. Joint Global Ocean Flux Study (U.S. JGOFS)
Ocean Time-series Sites (Ocean Time-series)
Principal Investigator:
Nicholas Bates (Bermuda Institute of Ocean Sciences, BIOS)
Co-Principal Investigator:
Rodney J. Johnson (Bermuda Institute of Ocean Sciences, BIOS)
Scientist:
Paul J. Lethaby (Bermuda Institute of Ocean Sciences, BIOS)
Claire Medley (Bermuda Institute of Ocean Sciences, BIOS)
Data Manager:
Dominic Smith (Bermuda Institute of Ocean Sciences, BIOS)
Version:
4
Version Date:
2024-05-03
Restricted:
No
Validated:
No
Current State:
Preliminary and in progress
HPLC and fluorometric derived phytoplankton pigment data from BATS Validation cruises for years 1996-2023
Abstract:
Data presented here are discrete bottle HPLC derived phytoplankton pigments and fluorometric chlorophyll-a for BATS validation (BVAL) cruises from June 1996 (BVAL cruise #16) through June 2023 (BVAL cruise #60). Water samples are typically collected from 7-12 depths in the upper 250 meters of the water column, and then filtered under low vacuum through a 25mm GF/F filter. The filter is then flash frozen in liquid nitrogen and stored at -80 degrees C. Shoreside, analysis is performed on an HPLC using a method modified by Dr. R. Bidigare from the Wright et al. (1991) procedure. This method identifies the pigments chlorophyll-c3, chlorophyll-c2, peridinin, 19’-butanoyloxyfucoxanthin, fucoxanthin, 19’-hexanoyloxyfucoxanthin, prasinoxanthin, diadinoxanthin, alloxanthin, diatoxanthin, lutein, zeaxanthin, chlorophyll-b, chlorophyll-a, divinyl chlorophyllide-a, alpha and beta carotene. Additionally, chlorophyll-a and phaeopigments are analyzed using a fluorometric assay.