Flow Cytometry (FCM) Data
Are you planning to contribute Flow Cytometry (FCM) data to BCO-DMO? Here are some community-generated best practice recommendations to consider when preparing your data for submission based on Ribalet et al. (2023), doi: 10.5281/zenodo.8329545.
We often receive data tables that include cell counts, features, biomass, identifications, etc. from FCM analysis. In addition to any data tables, we encourage you to also submit the raw .fcs files Flow Cytometry Standard (FCS), which we can attach as supplemental files to your dataset page.
FCS is a data file standard for the reading and writing of data from flow cytometry experiments. These files have been identified as important for data reuse. The FCS specification has traditionally been developed and maintained by the International Society for Advancement of Cytometry (ISAC).
Suggested Metadata
We encourage you to include the following details in the metadata (or data) of your BCO-DMO submission, where possible and applicable. These are the community-driven recommendations from "Towards a repository and reproducible tools for aquatic flow" (Ribalet et al., 2023), doi: 10.5281/zenodo.8329545).