| Laboratory results on Copepod nauplii and adult gut epifluorescence in samples collected from the San Francisco estuary in 2013 | Nikon model E400 epifluorescence microscope at 20X magnification, outfitted with a Nikon B-2A longpass filter cube (470EX/515LP, Nikon) and a custom bandpass filter cube (430EX/680EM, Omega Optical)
| Epifluorescence Microscope |
| Experimental results describing Pseudodiaptomus marinus gut area measured in pixels from San Francisco State University in 2013 | Nikon model E400 epifluorescence microscope at 20X magnification, outfitted with a Nikon B-2A longpass filter cube (470EX/515LP, Nikon) and a custom bandpass filter cube (430EX/680EM, Omega Optical).
| Epifluorescence Microscope |
| Laboratory results on Copepod gut fullness index measured in pixels from San Francisco State University in 2013 (Food Limitation in Copepod nauplii project) | Nikon model E400 epifluorescence microscope at 20X magnification, outfitted with a Nikon B-2A longpass filter cube (470EX/515LP, Nikon) and a custom bandpass filter cube (430EX/680EM, Omega Optical).
| Epifluorescence Microscope |
| Laboratory results on Copepod gut fullness using two tintinnid diets from San Francisco State University in 2013 (Food Limitation in Copepod nauplii project) | Nikon model E400 epifluorescence microscope at 20X magnification, outfitted with a Nikon B-2A longpass filter cube (470EX/515LP, Nikon) and a custom bandpass filter cube (430EX/680EM, Omega Optical).
| Epifluorescence Microscope |
| Microbial abundance, activity, and nutrient concentrations in Delaware Bay from R/V Hugh R. Sharp HRS090706DK in the Delaware Bay from July 2009 (Active bacteria in surface waters project) | | Epifluorescence Microscope |
| Nutrient concentrations and microbial counts from Niskin bottle collections on R/V Atlantic Explorer A1620 in the Bermuda Atlantic Time-series Study site from Sept 2016 | Used to enumerate bacterial abundance
| Epifluorescence microscope |
| Nutrient concentrations and microbial counts from Niskin bottle collections on R/V Atlantic Explorer A1703 in the Bermuda Atlantic Time-series Study site from Mar/April 2016 | Used to enumerate bacterial abundance
| Epifluorescence microscope |
| Bulk and AA d15N values for ala, glu, and phe from phytoplantkon and grazers grown in lab chemostats | The reactors were monitored daily for 12-15 days to ensure constant conditions, thus minimizing predator: prey isotopic mismatch, and samples were taken for cell abundances and biovolumes using an Elzone counter and epifluorescence microscopy.
| epifluorescence microscopy |
| Adenosine triphosphate and microbial biomass measurements from the Chesapeake Bay, sampled aboard RV Fay Slover on April 11, 2018 | | Olympus BC61 epifluorescence microscope |
| ARISA relative abundances (bacterial community structure), San Pedro Channel, 5 depths, 2014 (Bacterial, Archaeal, and Protistan Biodiversity project, Marine Viral Dynamics project) | | Epifluorescence Microscope |
| Bacterial cell counts and Dissolved Organic Carbon (DOC) measurements from R/V Atlantis AT32, AT34, AT38, and AT39-06 in the western North Atlantic Ocean (35°N to 57°N; 45°W) in Nov. 2015, May 2016, Sep 2017, Mar/Apr 2018 | For bacterial abundance estimates
| Olympus BX51 Epiflourescence Microscope |
| Discrete bottle samples collected at the Bermuda Atlantic Time-series Study (BATS) site in the Sargasso Sea from October 1988 through June 2024 | Bacterial enumeration methods include DAPI stained Epifluoresence Microscopy / Olympus AX70
| Olympus AX70 Fluoresence Microscope |
| Benthic foraminiferal abundances of Mud Patch multicores from R/V Endeavor EN524 in the continental shelf off New England; 40.43 N 70.5 W from May 2013 (OA, Hypoxia and Warming project) | To examine specimens
| Leica MZFLIII epifluorescence microscope |
| Survey biogeochemical data from R/V Atlantis AT32, AT34, AT38, and AT39-06 in the western North Atlantic Ocean (35°N to 57°N; 45°W) in Nov. 2015, May 2016, Sep 2017, Mar/Apr 2018 | For bacterial abundance estimates
| Olympus BX51 Epiflourescence Microscope |
| BIOS-SCOPE survey biogeochemical data as collected on Atlantic Explorer cruises (AE1614, AE1712, AE1819, AE1916) from 2016 through 2019 | Olympus BX51 epifluorescent microscope was used to obtain bacterioplankton abundance in 10^8 cells per kilogram.
| Olympus BX51 epifluorescent microscope |
| Carbonate chemistry, shell growth, and respiration data from laboratory experiments on California mussel larvae condcuted at the Hatfield Marine Science Center, Newport, OR in 2013 | Epifluorescent microscopy was used to determine the proportion of larvae feeding.
| epifluorescent microscope |
| Biovolume data from samples obtained on Gould cruise LMG1411 in the Western Antarctica Peninsula during 2014 (Polar Transcriptome project). | Used to estimate biovolume of diatoms
| Olympus BX61 Upright Wide Field Microscope |
| Chemistry and cell counts of formation fluids from North Pond, western flank of the Mid-Atlantic Ridge, from 2012-2014 | Total microbial biomass in fluids was enumerated with DAPI (4′,6′-diamidino-2-phenylindole; Sigma-Aldrich, St Louis, MO, USA) and epifluorescent microscopy (Porter and Feig, 1980).
| epifluorescent microscopy |
| Detection and Quantification of Cells Exhibiting 'Selfish' Uptake in the Western North Atlantic, in Danish Coastal Seawater, and Abyssopelagic Waters off the Eastern Coast of Japan Under Varying Hydrostatic Pressures, 2023-2024 | Zeiss AxioImager.Z2 microscope stand, Carl Zeiss - Fully automated epifluorescence microscope
| Zeiss AxioImager.Z2 microscope stand, Carl Zeiss - Fully automated epifluorescence microscope |
| Experimental results: coral calcification, chlorophyll-a content, algal density, energy reserves, and tissue biomass from samples of reef systems collected from northwest Fiji in 2011 | Endosymbiont quantification was calculated using 6 independent replicate counts on a hemocytometer, using a Nikon microphot-FXA epifluorescent microscope at 100X magnification.
| Nikon microphot-FXA epifluorescent microscope |
| Bacterioplankton data from coral and coral mucus aquaria experiments conducted at Bermuda Institute of Ocean Sciences in 2013 | Slides were enumerated using this microscope.
| AX70 epifluorescent microscope |
| Linear extension measurements of species from two common morphotypes of coralline algae found in Sitka Sound, AK. from 2017 - 2019 (High latitude kelp dynamics project) | To measure linear growth, the coralline algae were imaged using a fluorescent lamp channel on a Ziess AxioZoom microscope at the UCSC Microscopy Center.
| Ziess AxioZoom microscope |
| Physiological measurements during Cyanobacteria Crocosphaera iron/warming experiments | Olympus BX51 epifluorescence microscope
| Olympus BX51 epifluorescence microscope |
| CTD summary and prey size data for five mucous mesh grazer species collected during R/V Sikuliaq Cruise SKQ202204S and R/V Marcus G. Langseth Cruise MGL2207 in the Northern California Current in Mar and Jul 2022 | A small volume (~10 µl) of homogenized samples was imaged using bright field microscopy on a Nikon epifluorescence compound light microscope equipped with a Nikon D850 camera.
| Nikon Eclipse Ni epifluorescence microscope |
| 3A: Removal of organic carbon by natural bacterioplankton communities as a function of pCO2 from laboratory experiments between 2012 and 2016 | Samples for bacterioplankton abundance were analyzed by epifluorescence microscopy with 0, 6-diamidino -2-phenyl dihydrochloride (5µg mL-1, DAPI, SIGMA-Aldrich, St. Louis, MO, USA) according to Porter and Feig 1980.
| Epifluorescence microscopy |
| Growth rates for Emiliania huxleyi thermal response curve across 12 temperatures from 8.5-28.6C | Used to count cell samples
| Olympus BX51 microscope |
| Growth rates under low and high temperatures for Emiliania huxleyi in constant and fluctuating thermal environments | Used to count cell samples.
| Olympus BX51 microscope |
| Extracted chlorophyll data, particulate carbon and nitrogen, flow cytometry and 15N2/13C rate measurements collected during EN596 from April 2017 in the North Atlantic | Enumeration of diazotrophic taxa was performed using epifluorescence microscopy.
| epifluorescence microscopy |
| Bulk water cell abundance of samples taken aboard the R/V Endeavor EN638, May 2019 in the Northern Atlantic | An automated epifluorescence microscope (Zeiss AxioImager.Z2 microscope stand, Carl Zeiss Jena, Gemany) equipped with a cooled charged-coupled-device (CCD) camera (AxioCam MRm + Colibri LED light source, Carl Zeiss), a light-emitting diode for DAPI (UV-emitting LED, 365 nm) and a HE-62 multi filter module with a triple emission filter (425/50 nm, 527/54 nm, LP 615 nm, including a triple beam splitter of 395/495/610, Carl Zeiss)
| Zeiss Axio Imager Epifluorescence microscope |
| Epifluorescence Microscopy Water Column Samples from R/V Tangaroa TAN1810 in the Chatham Rise (Subtropical and Sub-Antarctic waters off of New Zealand) from October to November 2018 (Salp Food Web Ecology project) | Slides were calibrated from pixels to microns for both 0.8 µm and 8 µm to determine what length of pixels equates to µm thus only micron area, width and feret length are show in the raw datasheet. Our data also includes conversation ratios in the raw excel sheet. This ratio should be included when determining biomass in units of pg C/mL or abundance in #/mL, along with volume filter, as this ratio incorporates the area of the images taken and gives a value that states the ratio of the cells that were actually counted for 0.8 µm and 8 µm samples.
| Epifluorescence microscope: Olympus BX51 microscope with a Olympus DP72 camera and Exfo X-cite Series 120 mercury bulb with a FITC filter for green fluroescence |
| Estimated abundances of viruses and bacteria determined in samples collected in the Eastern Tropical North Pacific (ETNP) on R/V New Horizon cruise NH1315 during June 2013 | Epifluorescence microscope (Axio Imager. D2, Zeiss, Jena, Germany)
| Epifluorescence microscope |
| Otolith increment data from Stegastes partitus collected by SCUBA dives in the Upper Florida Keys, USA from 2003-2008 (FK Fish Recruitment project, FK Population Connectivity project) | The clearest lapillus was chosen from each individual and viewed under 400 magnification through a Leica DMLB microscope equipped with a polarized filter between the first stage and light source. The image was captured by a Dage MTI video camera and analyzed using Image Pro Plus 4.5 software (Media Cybernetics).
| Leica DMLB microscope |
| Summary of otolith microstructure data from Stegastes partitus collected by SCUBA dives in the Upper Florida Keys, USA from 2003-2008 (FK Fish Recruitment project, FK Population Connectivity project) | The clearest lapillus was chosen from each individual and viewed under 400 magnification through a Leica DMLB microscope equipped with a polarized filter between the first stage and light source. The image was captured by a Dage MTI video camera and analyzed using Image Pro Plus 4.5 software (Media Cybernetics).
| Leica DMLB microscope |
| Microbial cell counts derived from the formation fluids recovered from the CORKs installed at North Pond; collected on Maria S. Merian cruises MSM20-5 and MSM37 from 2012-2014 (North Pond Microbes project) | | epiflourescent microscopy |
| Geochemistry and microscopy from incubation experiments of Chesapeake Bay sediments conducted at Horn Point Laboratory | Zeiss Axiophot fluorescent microscope equipped with a digital Zeiss AxioCam MR camera, and Zen Pro software
| Zeiss Axiophot fluorescent microscope |
| Geochemistry summary data: single cell isotope incorporation of 1H, 2H, 12C14N, 12C15N, 12C12C, 12C13C ions from Chikyu-337 (IODP 337) | SYBR stained cells were imaged with a BX51 epifluorescence microscope (Olympus, Tokyo, Japan).
| BX51 epifluorescence microscope |
| Microzooplankton ingestion on low to high pCO2 acclimated E. huxleyi: Favella and Oxyrrhis as grazers (E Hux Response to pCO2 project) | epi-fluorescent microscope under blue-light excitation
| |
| Grazing experiment 4a: Short term data for low-high pCO2 acclimated Rhodomonas sp. cultures ingested by Gyrodinium grazers (E Hux Response to pCO2 project) | For cell counts
| epi-fluorescent microscope under blue-light excitation |
| Short term data for low-high pCO2 acclimated Rhodomonas sp. cultures ingested by Favella grazers (E Hux Response to pCO2 project) | For cell counts
| epi-fluorescent microscope under blue-light excitation |
| Grazing experiment 5:Long term microzooplankton ingestion and growth on low-high pCO2 acclimated Rhodomonas sp. cultures ingested by Oxyrrhis grazers (E Hux Response to pCO2 project) | For cell counts
| epi-fluorescent microscope under blue-light excitation |
| Grazing experiment 5: Short term microzooplankton ingestion on low-high pCO2 acclimated Rhodomonas sp. cultures ingested by Oxyrrhis grazers (E Hux Response to pCO2 project) | For cell counts
| epi-fluorescent microscope under blue-light excitation |
| Grazing experiment 6:Long term microzooplankton ingestion and growth on low-high pCO2 acclimated Rhodomonas sp. cultures ingested by Coxliella grazers (E Hux Response to pCO2 project) | For cell counts
| epi-fluorescent microscope under blue-light excitation |
| Grazing experiment 6: Short term microzooplankton ingestion on low-high pCO2 acclimated Rhodomonas sp. cultures ingested by Coxliella grazers (E Hux Response to pCO2 project) | For cell counts
| epi-fluorescent microscope under blue-light excitation |
| Grazing experiment 7:Long term microzooplankton ingestion and growth on low-high pCO2 acclimated Rhodomonas sp. cultures ingested by Gyrodinium grazers (E Hux Response to pCO2 project) | For cell counts
| epi-fluorescent microscope under blue-light excitation |
| Experimental results: Exopolymer and carbohydrate production by diatoms with growth; conducted at the Thornton lab, TAMU from 2007-2012 (Diatom EPS Production project) | Bacteria were counted and cell permeability was determined using an epifluorescence microscope (Axioplan 2, Carl Zeiss MicroImaging).
| Epifluorescence Microscope |
| Microsphere counts from Oikopleura dioica guts following feeding incubations with two microsphere sizes (for each functionalized microsphere) available in equal concentration. | Nikon Eclipse Ni epifluorescence microscope
| Nikon Eclipse |
| Microsphere counts from Oikopleura dioica guts following feeding incubations with three microsphere sizes (for each functionalized microsphere) available in environmentally relevant concentrations | Nikon Eclipse Ni epifluorescence microscope
| Nikon Eclipse |
| Symbiont removed and anemone reinfected with one of two strains of Symbiodinium from (AnemoneOA project) | [EVOS system, ThermoFisher (Life Technologies), Waltham, MA, USA; excitation: 628 ± 20 nm, emission: 692 ± 20 nm].
Field of view was determined using the EVOS operating software (4× objective), and cells were counted using the “Analyze Particles” tool in ImageJ (NIH, Bethesda, MD, USA).
| Fluorescence Microscope |
| Host-symbiont respiration related to symbiont density; anemones from Key Largo from (AnemoneOA project) | Used to visualize cells' chlorophyll a fluorescence. [EVOS system, ThermoFisher (Life Technologies), Waltham, MA, USA; excitation: 628 ± 20 nm, emission: 692 ± 20 nm].
| fluorescence microscope |
| Microsphere counts from Oikopleura dioica houses following feeding incubations with two microsphere sizes (for each functionalized microsphere) available in equal concentration. | Nikon Eclipse Ni epifluorescence microscope
| Nikon Eclipse |
| Microsphere counts from Oikopleura dioica houses following feeding incubations with three microsphere sizes (for each functionalized microsphere) available in environmentally relevant concentrations | Nikon Eclipse Ni epifluorescence microscope
| Nikon Eclipse |
| Microbial cell abundance,carbon fixation rates, and nitrate concentrations during shipboard incubations of vent fluids at the diffuse-flow vent Crab Spa, East Pacific Rise on RV/Atlantis cruise AT37-12, May 2017 | Used for microbial cell counts.
| |
| Initial prey abundances for copepod grazing experiments in the Kaneohe Bay, HI, May-June 2013 (MEPS 2017) (EAGER: Copepod nauplii project) | | |
| Supplementary Table 3B: Replicate cell counts for the 11 samples and alkaline phosphatase activity measurements available for any of the 11 samples | Cell counts performed with a Zeiss Axio Imager M2 Epifluorescence microscope.
| Zeiss Axio Imager M2 Epifluorescence microscope |
| Adenosine triphosphate and microbial biomass measurements from the Chesapeake Bay, sampled aboard RV Fay Slover on June 18, 2019 | | Olympus BC61 epifluorescence microscope |
| Lipid analysis data from experiment on grazing and physiological effects of ocean acidification on sand dollar larvae (Dendraster excentricus), July 2017 | Used to photograph larval intracellular lipid droplets.
| epi-fluorescent microscope (Leica 80i) |
| CARD FISH Eukaryote, Fungi, Labyrinthomycete, Kinetoplastid counts from MEDEA-II R/V Pelagia 64PE356 in the North Atlantic: Galway, Ireland to Reykjavik, Iceland from June to July 2012 | Olympus BX51 epifluorescence microscope, U-LH100HG APO mercury burner, 100 x UPlanSApo objective lens, and 20 x ocular magnification.
| |
| Microscopy data from B/O Hermano Gines cruises in the CARIACO Basin Time Series Station from May to November 2014 (CariacoMetaOmics project) | Cells and virus-like particles were enumerated using the appropriate filter set.
| Zeiss Axioscope epifluorescent microscope |
| Microbial diversity and geochemistry of marine sediment mesocosm, Cape Lookout Bight, North Carolina | | |
| Microscopy counts (Beggiatoa-like filaments, Cable bacteria), together with in situ temperature and salinity and surface sediment chlorophyll concentrations, of ex situ sediment cores collected in the Chesapeake Bay during 2017-2018 | | Zeiss Axio Imager 2 |
| Microsphere counts from incubation chambers of Oikopleura dioica prior to and following feeding incubations for experiment with artificial microspheres with two functionalized surfaces in three sizes | Nikon Eclipse Ni epifluorescence microscope
| Nikon Eclipse |
| Laboratory data on cell abundance of Minutocellus polymorphus in experiments measuring TEP and production of microaggregates | Single cell abundance of diatoms and bacteria were determined using epifluorescence microscopy
| AxioScope.A1 (Carl Zeiss, Germany) |
| Abundance of aggregates of the marine diatom Minutocellus polymorphus and particle-associated marine bacteria from culture and roller tank experiments in 2021-2022 | Single cell abundance of diatoms and bacteria were determined using epifluorescence microscopy
| Epifluorescence microscope |
| Biogeochemical and microbial field surveys from the BATS site, Bermuda from R/V Atlantic Explorer cruises from 2009-2013 (Ocean Microbial Observatory project) | for measuring bacterioplankton abundance
| |
| Virioplankton abundance using FISH probe at BATS site in the western Sargasso Sea from 2000-2011 (Ocean Microbial Observatory project) | Olympus AX70 microscope (Olympus, Tokyo, Japan) equipped with a Toshiba CCD video camera (Irvine, CA, USA)
| Epifluorescence Microscope |
| Virioplankton abundance from multiple cruises at the Bermuda AtlanticTime Series Station (BATS), Western Sargasso Sea from 2000-2011 (Ocean Microbial Observatory project) | Olympus AX70 microscope (Olympus, Tokyo, Japan) equipped with a Toshiba CCD video camera (Irvine, CA, USA)
| Epifluorescence Microscope |
| Single cell isotope incorporation of 1H, 2H, 12C14N, 12C15N. 12C12C, 12C13C from Chikyu-337 (IODP 337) | SYBR stained cells were imaged with a BX51 epifluorescence microscope (Olympus, Tokyo, Japan).
| BX51 epifluorescence microscope |
| Copepods Parvocalanus crassirostris and Bestiolina similis naupliar ingestion and clearance rates on natural prey assemblages from Kaneohe Bay, Oahu, 2013 (MEPS 2017) (EAGER: Copepod nauplii project) | For nano- and microplankton abundance.
| epifluorescence microscope |
| Results of experiments on feeding physiology of Mytilus californianus larvae in OA conditions | Larvae were crushed under a cover slip to flatten gut contents and allow better enumeration of all ingested beads in larvae under an epifluorescent microscope (objective 20x; Leica DM 1000).
| epifluorescent microscope |
| Near Edge X-ray Fluorescence Spectroscopy Data for standard organic phosphorus compounds | X-ray fluorescence microscope located at beamline 2-ID-B at the Advanced Photon Source, Argonne National Laboratory. The beamline is optimized to examine samples over a 1–4 keV energy range using a focused X-ray beam with a spot size of approximately 60 nanometers squared . The energy was calibrated using a NIST standard reference material SRM2910 calcium hydroxyapatite. The whiteline energy of the standard was set to 2153 eV.
| X-ray fluorescence microscope |
| Experimental results: Exopolymer production by phytoplankton under oxidative stress; conducted at the Thornton lab, TAMU from 2007-2012 (Diatom EPS Production project) | Cell permeability was determined using an epifluorescence microscope (Axioplan 2, Carl Zeiss MicroImaging).
| Epifluorescence Microscope |
| Physiological response of eight Palau coral colonies to thermal stress as seen in temperature experiments in 2014 and 2015 | | EVOS digital fluorescent microscope |
| Plankton abundances from phytoplankton experiments on the RVIB Nathaniel B. Palmer NBP0608 cruise in the Ross Sea, Southern Ocean during 2006(CORSACS project, Antarctic microzooplankton project) | | Microscope-Fluorescence |
| Cell abundance of xenic and axenic marine Prochlorococcus and Synechococcus cultures from laboratory experiments conducted in 2016 and 2017 | AxioScope.A1, Carl Zeiss, Germany
| AxioScope.A1 |
| Growth rates of xenic and axenic marine Prochlorococcus and Synechococcus cultures from laboratory experiments conducted in 2016 and 2017 | | AxioScope.A1, Carl Zeiss, Germany |
| Plankton size spectra compiled from projects CCE LTER, HOT, CRD FluZIE, BLOOFINZ-GoM, and SalpPOOP in the California Current Ecosystem, North Pacific subtropical gyre, Costa Rica Dome, Gulf of Mexico, and Southern Ocean subtropical front from 2004-2018 | A ZeissAxioVert200M microscope was used for most of the microscopy, and an Olympus BX51 microscope was used for samples from the SalpPOOP cruise (TAN1810).
| Fluorescence Microscope |
| Data from an experiment that measured the occurrence of feeding among 4 Prorocentrum minimum strains on the cryptophyte Teleaulax amphoxeia | | |
| Data from an experiment that measured the occurrence of feeding among 8 Prorocentrum minimum strains on fluorescently labeled bacteria or the cryptophyte Teleaulax amphioxeia | | |
| Potential microplastic counts of 6 common Caribbean spong species collected June 21, 2019 in Bocas del Toro, Panama | All filters were analyzed for PMP presence using an E600 Nikon Eclipse fluorescence microscope fitted with a UV-1A fluorescence filter block (EX 360–370, DM 400, BA 400).
| E600 Nikon Eclipse fluorescence microscope |
| Potential microplastic sizes of 6 common Caribbean spong species collected June 21, 2019 in Bocas del Toro, Panama | All filters were analyzed for PMP presence using an E600 Nikon Eclipse fluorescence microscope fitted with a UV-1A fluorescence filter block (EX 360–370, DM 400, BA 400).
| E600 Nikon Eclipse fluorescence microscope |
| Protist numbers in subtropical North Atlantic at and below 100 m collected on the R/V Pelagia (64PE356) from Galway, Ireland to Reykjavik, Iceland in 2010 (Eukaryote Microbes NAtl project) | Epifluorescence microscope (Olympus BX51)
| |
| Protist carbon from microscopy samples collected in the Argo Basin of the Eastern Indian Ocean on R/V Roger Revelle cruise RR2202 from Feb to Mar 2022 | Epifluorescence microscope: Olympus BX51 microscope with a Olympus DP72 camera and Exfo X-cite Series 120 mercury bulb with a FITC filter for green fluorescence.
| Olympus BX51 microscope with a Olympus DP72 camera |
| Protist numbers at and below 100 m from R/V Pelagia 64PE280, 64PE325 in the tropical and subtropical N. Atlantic from 2007-2010 (Basin-scale Protists project) | Olympus BX51 and BX50 epifluorescence microscopes | Fluorescence Microscope |
| Reconstructed genomes from North Pond, western flank of the Mid-Atlantic Ridge, from 2012-2014 | Total microbial biomass in fluids was enumerated with DAPI (4′,6′-diamidino-2-phenylindole; Sigma-Aldrich, St Louis, MO, USA) and epifluorescent microscopy (Porter and Feig, 1980).
| epifluorescent microscopy |
| Bulk and cell-specific CO2 fixation and PO4 uptake from Atlantic Explorer cruise AE1524 (BATS validation cruise BV50), September 2015 | Used to count calibration beads.
| Epifluorescence microscope |
| Sequence data accession numbers originating from coral and coral mucus mesocosm experiments conducted at the Bermuda Institute of Ocean Sciences in 2013 | Slides were enumerated using this microscope.
| AX70 epifluorescent microscope |
| Shotgun Proteomics of Bering Sea algal incubations. | | |
| SPOT Microbial Observatory Protist 18S rDNA TRFLP relative peak abundance data from 2014 (Bacterial, Archaeal, and Protistan Biodiversity project, Marine Viral Dynamics project, Mar. Microbial Communities project) | | |
| SPOT Microbial Observatory Myovirus g23 3' TRFLP relative peak abundance data from 2014 (Bacterial, Archaeal, and Protistan Biodiversity project, Marine Viral Dynamics project, Mar. Microbial Communities project) | | |
| SPOT Microbial Observatory Myovirus g23 5' TRFLP relative peak abundance data from 2014 (Bacterial, Archaeal, and Protistan Biodiversity project, Marine Viral Dynamics project, Mar. Microbial Communities project) | | |
| SPOT Microbial Observatory Bacterial ARISA relative peak abundance data from 2014 (Bacterial, Archaeal, and Protistan Biodiversity project, Marine Viral Dynamics project, Mar. Microbial Communities project) | | |
| Environmental data for SPOT virus sampling in Southern California during 2014 (Bacterial, Archaeal, and Protistan Biodiversity project) | | |
| Microbial cell counts in sediment cores collected during R/V Knorr cruise KN195-03 in the Pacific Ocean in 2009 (Subseafloor Microbial Life project) | Direct cell counts were obtained by placing a small aliquot of the slurry directly on a 0.2-um pore size filter and enumerating manually under a fluorescence microscope.
| fluorescence microscope |
| Effects of temperature on biomass and sulfide production in Desulfovibrio hydrothermals (INSPIRE_Pyrite project) | Light microscope equipped for bright field (both transmitted and reflected), phase contrast, transmitted and reflected polarization and Nomarski differential interference contrast microscopy, as well as epifluorescence.
| Epifluorescence Microscope - Zeiss Axiophot |
| Sulfur Near Edge X-ray Fluorescence Spectroscopy Data for standard materials | X-ray fluorescence microscope located at beamline 2-ID-B at the Advanced Photon Source, Argonne National Laboratory: The beamline is optimized to examine samples over a 1–4 keV energy range using a focused X-ray beam with a spot size of approximately 60 nanometers squared . The energy was calibrated using an elemental sulfur standard. The whiteline energy of the elemental sulfur standard was aligned to 2472 eV.
| X-ray fluorescence microscope |
| Traits of five Symbiodinium genotypes measured at three different nitrogen levels | Used to count cells for growth rate calculations.
| microscope |
| Results from growth rate experiment with the diatom Thalassiosira wessiflogii in semi-continuous culture; conducted at the Thornton lab, TAMU from 2007-2012 (Diatom EPS Production project) | Bacterial abunance and cell permeability were determined using an epifluorescence microscope (Axioplan 2, Carl Zeiss MicroImaging).
| Epifluorescence Microscope |
| Nutrient transfer experiments with host coral and symbionts under varying environmental conditions conducted March 2014 and March 2015 | Algal densities were quantified using an EVOS digital fluorescent microscope.
| EVOS digital fluorescent microscope |
| Partial SSU rRNA genes of bacteria and archaea from reef seawater samples produced using 515F/806R and 515F/806RB primers from the Bermuda, Red Sea, and Federated States of Micronesia in 2013 (Coral Microbial Relationships project) | AX70 epifluorescent microscope (Olympus, Tokyo, Japan)
| |
| Viral and bacterial counts from filtered water, stained with SYBR Green and counted using epifluorescent microscopy, from samples collected on R/V Knorr cruise KN210-04 in the Western Atlantic Ocean in 2013 (Deep Atlantic DOM project) | | Epifluorescent microscope |
©2020 Biological and Chemical Oceanography Data Management Office.