Instrument: Titrator

Acronym: Titrator
External Identifier: skos:broadMatch: http://vocab.nerc.ac.uk/collection/L05/current/LAB12/

Description

Titrators are instruments that incrementally add quantified aliquots of a reagent to a sample until the end-point of a chemical reaction is reached.


Dataset NamePI-Supplied DescriptionPI-Supplied Name
DIC, TA, calculated pH and carbonate saturation state in the summer bottom water in North Gulf of Mexico from 2006 to 2017AS-ALK2 is designed for both laboratory and shipboard use with small sample volume (10 – 25 mL) and high precision (0.1% or better). The titrator is fully automated with standardization and sample analysis. AS-ALK2 provides Gran titration for alkalinity determination of both seawater and brackish waters. Full temperature control is provided on all liquids. Water jackets are used to surround the digital syringes for temperature control. For best data quality, a water bath must be used to supply water with a constant temperature to control the titration temperature. Semi-automated titrator (AS-ALK2 Apollo Scitech)
Data from two Antarctic krill (Euphausia superba) 24-hour feeding experiments under ambient temperature and pCO2, ambient temperature and elevated pCO2, and elevated temperature and pCO2.Instrument used for open-cell titrations to determine total alkalinity in seawater. Used to measure total alkalinity in seawater. Metrohm 888 Titrando
Growth of Antarctic krill (Euphausia superba) from a 1-month experiment at ambient conditions, ambient temperature and elevated pCO2, or elevated temperature and elevated pCO2, Jan-Feb. 2014Instrument used for open-cell titrations to determine total alkalinity in seawater. Used to measure total alkalinity in seawater. Metrohm 888 Titrando
Growth of Antarctic krill (Euphausia superba) from a 1-month experiment at ambient conditions, ambient temperature and elevated pCO2, or elevated temperature and elevated pCO2, Jan-Feb. 2015Instrument used for open-cell titrations to determine total alkalinity in seawater. Used to measure total alkalinity in seawater. Metrohm 888 Titrando
Growth of Antarctic krill (Euphausia superba) from an experiment at two temperatures and three pH levels, Feb-Mar. 2015Instrument used for open-cell titrations to determine total alkalinity in seawater. Used to measure total alkalinity in seawater. Metrohm 888 Titrando
Temperature and pCO2 effects on survivability of 25 genotypes of Acropora cervicornis coral at Mote Marine Laboratory in Nov-Dec 2019Total Alkalinity (TA) was analyzed using the Metrohm 905 Titrando analyzer.  Metrohm 905 Titrando analyzer
Aquaria water quality total alkalinity, DIC, and CO2 measurements from full factorial study of Acropora cervicornis at Mote Marine Laboratory in Nov-Dec 2019Total Alkalinity (TA) was analyzed using the Metrohm 905 Titrando analyzer.  Metrohm 905 Titrando analyzer
Acropora cervicornis growth rates under different pH and temperature treatments from experiments at Summerland Key, Florida in September of 2016Metrohm 807 Titrando
Experimental results of calcification rates of algae at variable pCO2 concentrations from the Richard B Gump Research Station, Moorea, French Polynesia in 2011 (MCR LTER project, OA_Corals project)Automatic titrator
Hydrographic, nutrient, and carbonate system data from R/V Janan cruises in the Arabian Gulf in December 2018 and May 2019Total Alkalinity (μmol kg-1) was determined through open-cell automated titration (876 Dosimat plus, Metrohm AG) Metrohm AG 876 Dosimat plus
CO2, temperature, and oxygen effects on Atlantic silverside metabolic ratesG20 Potentiometric Titrator, Mettler Toledo®, Columbus, OH, USA.  Alkalinity titrator
Seawater biogeochemistry inshore and offshore the Bermuda coral reef from the Bermuda coral reef platform and adjacent Sargasso Sea extending to Bermuda Atlantic Time Series-station from 2014-2018Automated Winkler titration system using an UV light endpoint detection system as designed by the late Robert Williams of SIO. Precision was typically Automated Winkler titration system
Seawater chemistry and benthic metabolic rates as a function of coral cover from a mesocosm experiment conducted at the Bermuda Institute of Ocean Sciences in August 2012The open-cell potentiometric acid titration system was developed by the laboratory of A.G. Dickson. Briefly, a known amount of seawater is added to an open cell beaker where hydrochloric acid as acidified to a pH of 3.5-4.0 and allowed to stabilize to remove CO2 gas formed by the addition of acid. Small aliquots of hydrochloric acid are then added to pH of ~ 3.0. The titration is monitored by a glass electrode and the total alkalinity of the sample is calculated using a non-linear least-squares method following Dickson et al. (2007). Open-cell potentiometric acid titration system
Seawater chemistry and benthic metabolic rates as a function of coral cover from a mesocosm experiment conducted at the Hawaii Institute of Marine Biology in June 2016The open-cell potentiometric acid titration system was developed by the laboratory of A.G. Dickson. Briefly, a known amount of seawater is added to an open cell beaker where hydrochloric acid as acidified to a pH of 3.5-4.0 and allowed to stabilize to remove CO2 gas formed by the addition of acid. Small aliquots of hydrochloric acid are then added to pH of ~ 3.0. The titration is monitored by a glass electrode and the total alkalinity of the sample is calculated using a non-linear least-squares method following Dickson et al. (2007). Open-cell potentiometric acid titration system
Bottle chemistry data from hydrothermal vents sampled by CTD rosette during R/V Atlantis cruise AT37-12 in April and May 2017Custom Alkalinity Titrator
Bottle data and chemical analysis from Falkor cruise FK180624 in the Eastern Tropical North Pacific Ocean in 2018Custom total alkalinity titrator built by the lab of Andrew Dickson, UCSD-Scripps Custom total alkalinity titrator
Bottle data from R/V Endeavor cruise EN665 in the Gulf of Maine, conducted April 7-12, 2021Total alkalinity was determined using an open-system Gran titration on 5-mL samples in triplicate, using a Metrohm 805 Dosimat and a robotic Titrosampler, calibrated against seawater Certified Reference Materials. Titrosampler
Seawater carbonate chemistry from experiment on brooded coral larval, June and July 2010, Taiwan (Cumbo, 2012)(MCR LTER project, Climate_Coral_Larvae project)Model DS50, Mettler-Toledo, Columbus, OH, USA Automatic titrator
Seawater carbonate chemistry for brooded coral larval experiments, Taiwan, March 2011 and 2012 (Cumbo, 2013)(MCR LTER project, Climate_Coral_Larvae project)Model DL50, Mettler-Toledo, Columbus, OH, USA Automatic titrator
Porites coral calcification responses to declining Ωar in a CO2 manipulation experiment in Palau versus the calcification responses observed in ten other studies of massive Porites coralsAutomated gran titrations for TA were run on duplicate 1 ml samples using a Metrohm Titrando 808 and 730 Sample Changer. Metrohm Titrando 808 and 730 Sample Changer
Calcification data from the coral A. pulchra and alga L. kotschyanum under 2 pCO2 levels, 5-6 temperature treatments, and two different seasons (austral summer and spring) in 2010 (OA coral adaptation project)T50, Mettler-Toledo, Switzerland
Calcification rates of Porites corals collected from a naturally high-Ωar reef and a naturally low-Ωar reef in Palau incubated at three experimental Ωar conditionsAutomated gran titrations for TA were run on duplicate 1 ml samples using a Metrohm Titrando 808 and 730 Sample Changer. Metrohm Titrando 808 and 730 Sample Changer
Calcification rates of communities and sediment in a flume from Richard B Gump Research Station - Moorea LTER, French Polynesia from 2013 (MCR LTER project, OA_Corals project)A Mettler-Toledo T50 model automatic titrator was employed to analyze samples for total alkalinity using open cell, potentiometric titration. automatic titrator
Pore water data from sediment cores collected from R/V Oceanus cruise OC1906A and R/V Sikuliaq cruise SKQ202016S off the coast of California in 2019 and 2020used to measure alkalinity and initial pH Metrohm automatic titrator (model 785 DMP Titrino)
Carbonate chemistry data describing the behavior of coral larvae in high pCO2 within shallow tropical reefs in Okinawa, Japan from 2016-07 to 2016-08The salinity of the seawater used to fill the larval incubation tubes was measured using a conductivity meter (TetraCon 325, WTW, Germany), and AT was determined using open-cell titrations conducted with an autoburette titrator (Kimoto, ATT-05, Japan). autoburette titrator
Carbon chemistry (TCO2, TALK, POC, PON, dC13_POM, dN15_POM) from CTD bottles from RVIB Nathaniel B. Palmer cruise NBP1302 in the Ross Sea, Antarctica from February to March 2013 (TRACERS project)potentiometric titrator (Metrohm 855 Robotic Titrosampler)
Carbonate chemistry associated with copepod experiments with varying CO2 (OA-Copepod_PreyQual project)To measure alkalinity Titrando 888
P17N Ocean Acidification Pteropod Study: Carbonate chemistry system and hydrography samples from R/V New Horizon NH1208 from a transect 35-50 degrees North, Pacific Ocean, Aug-Sep 2012 (OAPS project)Apollo SciTech alkalinity auto-titrator (Model AS-ALK2): acid-base titrator, which automates the Gran titration procedure to measure alkalinity.
Carbonate chemistry parameters in flume study during the day from Richard B Gump Research Station - Moorea LTER, French Polynesia from 2013 (MCR LTER project, OA_Corals project)A Mettler-Toledo T50 model automatic titrator was employed to analyze samples for total alkalinity using open cell, potentiometric titration. automatic titrator
Carbonate chemistry data from static and fluctuating pCO2 x dissolved oxygen (DO) experiments on Menidia menidiaAlkalinity – Metler Toledo G20 Potentiometric Titrator calibrated with certified reference material from Dr. Andrew Dickson, University of California San Diego Metler Toledo G20 Potentiometric Titrator
Carbonate chemistry in Mission Aransas Estuary from May 2014 to Feb 2017 and Dec 2018 to Feb 2020AS-Alk2 alkalinity titrator, Apollo SciTech Inc
Carbonate chemistry in Mission Aransas Estuary from May 2014 to Feb 2017 and Dec 2018 to Feb 2020Metrohm Titrando 888, calcium ion-selective Titrode
Carbonate chemistry and isotopes from multiple M/V OOCL Tianjin and M/V OOCL Tokyo cruises between Hong Kong and Long Beach in the Pacific Basin from 2008-2012 (NPac Cont Ship project)To measure total alkalinity automated, open-cell potentiometric titration system
Carbonate chemistry and light data from bottle samples Total alkalinity (TA) samples were analyzed via closed cell potentiometric titrations using a VINDTA 3S system. VINDTA (Versatile INstrument for the Determination of Titration Alkalinity). The system uses a Metrohm Titrino 719S, an ORION-Ross pH electrode and a Metrohm reference electrode. The burette, the pipette and the analysis cell have a water jacket around them. Precision is typically +/- 1 umol/kg for TA. Please see for a complete instrument description. MARIANDA VINDTA 3S
Water samples from CTD casts and vehicle-mounted bottles from the R/V Atlantis, R/V Ronald Brown, R/V Falkor, & E/V Nautilus in the Gulf of Mexico & Florida from 2010 to 2014 (Lophelia OA project)Measured total alkalinity Mettler-Toledo EL15 autotitrator
Experimental results of effects of acidification on corals and calcified algae collected from the Richard B Gump Research Station during 2011, in French Polynesia (MCR LTER project, OA_Corals project)A Mettler-Toledo T50 model automatic titrator was employed to analyze samples for total alkalinity using open cell, potentiometric titration. automatic titrator
Chemistry and cell counts of formation fluids from North Pond, western flank of the Mid-Atlantic Ridge, from 2012-2014Fluids also were analyzed for dissolved silicon and nitrate using automated colorimetric analysis and pH was measured with an electrode before a potentiometric titration for the determination of alkalinity (Wheat et al., 2017). automated colorimetric analysis
Data on chorion thickness from embryos studied in an experiment on CO2 sensitivity of Northern sand lance (Ammodytes dubius) embryos conducted in 2018Mettler Toledo G20 Potentiometric Titrator
CO2 × temperature specific early life survival and growth of Menidia menidia assessed by 5 factorial experimentsBottles were stored at 3˚C and measured for AT within two weeks of sampling using an endpoint titration (Mettler Toledo G20 Potentiometric Titrator).  Mettler Toledo G20 Potentiometric Titrator
Coral (Porites rus) calcification and chemistry data from outdoor flumes at the UCB Gump Research Station Moorea, French Polynesia in April and March of 2012TA: Mettler Toledo T50 Mettler Toledo T50
TA: Mettler Toledo T50  Mettler Toledo T50
Calcification data from outdoor flume experiments with coral Acropora hyacinthus at the UCB Gump Research Station Moorea, French Polynesia in September and October of 2012TA: Mettler Toledo T50 Mettler Toledo T50
Carbonate chemistry from outdoor flume experiments with coral Acropora hyacinthus at the UCB Gump Research Station Moorea, French Polynesia in September and October of 2012TA: Mettler Toledo T50  Mettler Toledo T50
Calcification and carbonate chemistry from outdoor flume experiments with corals at locations in Moorea, Okinawa, and Hawaii from 2011 to 2013TA: Mettler Toledo T50 Mettler Toledo T50
Net calcification rates of coral and calcified algae in pCO2 flume experiments in Moorea, French Polynesia from August to October of 2014 (OA_Coral_adapt project, MCR_LTER project)Automatic Titrator: T50, Mettler-Toledo, Switzerland Titrator: T50
TA: Mettler Toledo T50 Mettler Toledo T50
TA: Mettler Toledo T50 Mettler Toledo T50
TA: Mettler Toledo T50 Mettler Toledo T50
Results from experiment examining effects of 4 different dyes on growth rates of scleractinian corals; from the Cohen lab at WHOI in Woods Hole, MA (OA Nutrition and Coral Calcification project)Alkalinities were measured via titration with 0.01 N HCl containing 40.7 g NaCl/l using a Metrohm Titrando 808 Dosimat and a 730 Sample Changer controlled by Tiamo software to performautomated normalized Gran titrations of 1 ml samples. Titrator
Physiological parameters of three corals species collected from Hawaii and exposed to four treatment conditions for 22-months as part of a mesocosm experimentTotal alkalinity was measured using an automatic titrator (Titrino© Plus 877, Metrohm) with pH glass electrode (9101 Herisau, Metrohm©). Titrino Plus 877, Metrohm
Carbonate chemistry measurements from coral-light experiments (Dufault, 2013, JEB), Taiwan 2010 (MCR LTER project, Climate_Coral_Larvae project)Model DS50, Mettler-Toledo, Columbus, OH, USA Automatic titrator
Total alkalinity incubation data for coralline algae from August to September 2017 at the Sitka Sound Science Center (SSSC) (High latitude kelp dynamics project)TA measurements were performed using open cell titration (Metrohm, 905 Titrandro) and corrected against certified reference materials of CO2 in seawater (Dickson laboratory, Scripps Institution of Oceanography), with an average standard error of ±0.933μmol kg-1 SW-1 among sample triplicates. Metrohm 905 Titrandro titrator
Environmental data from experimental aquaria during a laboratory experiment testing the effects of pH, light availability and biotic interaction on coralline algae calcification and productivity at the Sitka Sound Science Center in 2017Total alkalinity (TA )measurements were performed using open cell titration and corrected against certified reference materials of CO2 in seawater (Dickson laboratory, Scripps Institution of Oceanography), with an average standard error of ±0.933μmol kg-1 SW-1 among sample triplicates. Metrohm 905 Titrandro Titrator
Experimental results of calcification rates of corals at variable pCO2 from the Richard B Gump Research Station, Moorea, French Polynesia in 2011 (MCR LTER project, OA_Corals project)Automatic titrator
Results of laboratory study examining effects of elevated concentrations of seawater carbon dioxide and altered salinity on rates of oxygen utilization by larval porcelain crabs (Petrolisthes cinctipes)Total alkalinity (TA) was measured by autotitration (Metrohm 809; Metrohm, Herisau, Switzerland). Metrohm 809
Results of laboratory study examining effects of elevated concentrations of seawater carbon dioxide and altered salinity on rates of oxygen utilization by larval porcelain crabs (Petrolisthes cinctipes)Total alkalinity (TA) was measured by autotitration (Metrohm 809; Metrohm, Herisau, Switzerland). Metrohm 809
Results of laboratory study examining effects of elevated concentrations of seawater carbon dioxide and altered salinity on rates of oxygen utilization by larval porcelain crabs (Petrolisthes cinctipes)Total alkalinity (TA) was measured by autotitration (Metrohm 809; Metrohm, Herisau, Switzerland). Metrohm 809
Results of laboratory study examining effects of elevated concentrations of seawater carbon dioxide and altered salinity on rates of oxygen utilization by larval porcelain crabs (Petrolisthes cinctipes)Total alkalinity (TA) was measured by autotitration (Metrohm 809; Metrohm, Herisau, Switzerland). Metrohm 809
Experiment with the diatom Chaetoceros sp. on the impact of temperature, light climate, and carbonate chemistry on TEP production and aggregation processes from May 2015 (OA - Effects of High CO2 project)TA samples were measured following best practices (Dickson et al., 2007) on an Apollo SciTech (AS-ALK2) using certified titrant and certified reference materials purchased from Andrew Dickson at Scripps Institute of Oceanography. Apollo SciTech (AS-ALK2)
Diel carbonate and nutrient chemistry reported for different benthic reef communities under ambient and acidified conditions during summer and winterThe open-cell potentiometric acid titration system was developed by the laboratory of A.G. Dickson. Briefly, a known amount of seawater is added to an open cell beaker where hydrochloric acid as acidified to a pH of 3.5-4.0 and allowed to stabilize to remove CO2 gas formed by the addition of acid. Small aliquots of hydrochloric acid are then added to pH of ~ 3.0. The titration is monitored by a glass electrode and the total alkalinity of the sample is calculated using a non-linear least-squares method following Dickson et al. (2007). Open-cell potentiometric acid titration system
Dissolved and particulate carbon and nitrogen data from seawater collected during CCGS John P. Tully cruises in the northeast Pacific Ocean from Vancouver Island to Station P from 2018 to 2020The average accuracy of the Metrohm 855 automated titrator relative to Certified Reference Materials is better than 1μmol per kilogram.  Metrohm 855 automated titrator
Seawater chemistry treatment conditionsMettler Toledo T50
Chemical analysis from sediment core bottom water samples collected in the back barrier island bays on Virginia’s Eastern Shore in July 2017Used to measure alkalinity and pH Metrohm automatic titrator (model 785 DMP Titrino)
Chemical analysis from sediment core pore water samples collected in the back barrier island bays on Virginia’s Eastern Shore in July 2017Used to measure alkalinity and pH. Metrohm automatic titrator (model 785 DMP Titrino)
Sediment core sample metadata collected July 2017 in the back barrier island bays on Virginia’s Eastern ShoreUsed to measure alkalinity and initial pH Metrohm automatic titrator (model 785 DMP Titrino)
Carbonate data in experimental treatments of Emiliania huxleyi, 2011-2012 (E Hux Response to pCO2 project)Metrohm 888 Titrando Automatic titrator
Experimental results: Chlorophyll-a, POC, and cell volume of E. huxleyi at 3 pCO2 levels, 2011-2012 (E Hux Response to pCO2 project)Metrohm 888 Titrando with a Metrohm Ecotrode combined electrode, calibrated with TRIS and AMP buffers on the total H+ ion pH scale. Automatic titrator
Carbon and nitrogen content of E. huxleyi at 3 pCO2 levels, 2011-2012 (E Hux Response to pCO2 project)Metrohm 888 Titrando with a Metrohm Ecotrode combined electrode, calibrated with TRIS and AMP buffers on the total H+ ion pH scale. Automatic titrator
Experimental results: Emiliania huxleyi growth rates under different pCO2 levels from Bellingham, WA from 2011-2012 (E Hux Response to pCO2 project)Metrohm 888 Titrando with a Metrohm Ecotrode combined electrode, calibrated with TRIS and AMP buffers on the total H+ ion pH scale. Automatic titrator
Experimental results: cellular particulate DMSP from E. huxleyi at 3 pCO2 levels, 2011-2012 (E Hux Response to pCO2 project)Metrohm 888 Titrando with a Metrohm Ecotrode combined electrode, calibrated with TRIS and AMP buffers on the total H+ ion pH scale. Automatic titrator
Experimental results: Emiliania huxleyi growth rates under different pCO2 levels, 2011-2012 (E Hux Response to pCO2 project)Metrohm 888 Titrando with a Metrohm Ecotrode combined electrode, calibrated with TRIS and AMP buffers on the total H+ ion pH scale. Automatic titrator
SEM based morphology of Emiliania huxleyi, 2011-2012 (E Hux Response to pCO2 project)Metrohm 888 Titrando Automatic titrator
Carbonate chemistry analyses (total alkalinity, DIC, and d13C of DIC) from discrete bottle samples and CTD data from 12 stations sampled during R/V Endeavor cruise EN669 in the Gulf of Maine during August 2021Total alkalinity was determined using an open-system Gran titration on 5-mL samples in triplicate, using a Metrohm 805 Dosimat and a robotic Titrosampler, calibrated against seawater Certified Reference Materials. Titrosampler
Growth metrics of Pocillopora verrucosa and Acropora hyacinthus corals under two pCO2 treatments at Moorea, French Polynesia from May to June of 2014 (OA coral adaptation project)Automatic Titrator: T50, Mettler-Toledo, Switzerland T50
Seawater chemistry during pCO2 flume experiments at Richard B Gump Research Station in Moorea, French Polynesia from May to June of 2014T50, Mettler-Toledo, Switzerland T50
Fatty acid profiles of M. menidia females and their unfertilized eggs.Used to measure total alkalinity Mettler Toledo™ G20 Potentiometric Titrator
Data from the fecundity trial in a study of CO2 and temperature-specific reproductive traits in Menidia menidiaG20 Potentiometric Titrator (Mettler Toledo)
Egg measurements from the fecundity trial in a study of CO2 and temperature-specific reproductive traits in Menidia menidiaG20 Potentiometric Titrator (Mettler Toledo)
Carbonate chemistry data for low-high pCO2 acclimated E. huxleyi cultures (E Hux Response to pCO2 project)Titrando 888
Grazing experiment 4: Carbonate chemistry data for low-high pCO2 acclimated Rhodomonas sp. cultures, 2011-2016 (E Hux Response to pCO2 project)Titrando 888
Grazing experiment 5: Carbonate chemistry data for low-high pCO2 acclimated Rhodomonas sp. cultures, 2011-2016 (E Hux Response to pCO2 project)Titrando 888
Grazing experiment 5: Carbonate chemistry of Rhodomonas for low-high pCO2 long term experiment treatments, 2011-2016 (E Hux Response to pCO2 project)Titrando 888
Grazing experiment 6: Carbonate chemistry data for low-high pCO2 acclimated Rhodomonas sp. cultures and long term grazing treatments (E Hux Response to pCO2 project)Titrando 888
Grazing experiment 7: Carbonate chemistry data for low-high pCO2 acclimated Rhodomonas sp. cultures and long term grazing treatments (E Hux Response to pCO2 project)Titrando 888
Growth data from static and fluctuating pCO2 x dissolved oxygen (DO) experiments on Menidia menidiaAlkalinity – Metler Toledo G20 Potentiometric Titrator calibrated with certified reference material from Dr. Andrew Dickson, University of California San Diego Metler Toledo G20 Potentiometric Titrator
Total dissolved Cobalt and labile Cobalt concentrations from R/V Knorr cruises KN199-04 and KN204-01 in the Subtropical northern Atlantic Ocean from 2010-2011 (U.S. GEOTRACES NAT project)Standard additions were carried out with Metrohm 765 Dosimats using a programmed dosing procedure (Noble and Saito et al. 2008). Automatic titrator
Inorganic Carbon along US GEOTRACES North Atlantic Transect from R/V Knorr KN199-04, KN204-01 cruises in the subtropical N. Atlantic from 2010-2011 (U.S. GEOTRACES NAT project)TA was determined by a high- precision (Automatic titrator
Chemical analysis from sediment core bottom water samples collected in the northern Gulf of Mexico, May 2017Used to measure alkalinity
Chemical analysis from sediment core bottom water samples collected in the northern Gulf of Mexico, May 2017Used to measure alkalinity and initial pH. Metrohm automatic titrator (model 785 DMP Titrino)
Chemical analysis from sediment core pore water samples collected in the northern Gulf of Mexico, May 2017Used to measure alkalinity and initial pH. Metrohm automatic titrator (model 785 DMP Titrino)
Sediment core sample metadata collected in the northern Gulf of Mexico, May 2017Used to measure alkalinity and initial pH. Metrohm automatic titrator (model 785 DMP Titrino)
Hatch data from experiments on CO2 sensitivity of Northern sand lance (Ammodytes dubius) embryos conducted in 2018 and 2020Mettler Toledo G20 Potentiometric Titrator
Carbonate Chemistry Parameters from a heatwave experiment done September to November 2018 using reef building corals collected in Kāne'ohe Bay, O'ahu, Hawai'i.Mettler Toledo T50
Experiment Tank Conditions from a heatwave experiment done September to November 2018 using reef building corals collected in Kāne'ohe Bay, O'ahu, Hawai'i.Mettler Toledo T50
Spatial surveys of carbonate chemistry in Heron Reef, Great Barrier Reef, AustraliaThe open-cell potentiometric acid titration system was developed by the laboratory of A.G. Dickson. Briefly, a known amount of seawater is added to an open cell temperature controlled beaker. Hydrochloric acid (0.1 N) is added using a Methrom Dosimat to a pH of 3.5-4.0. Following equilibration, small increments of hydrochloric acid are then added to the seawater until pH equals ~ 3.0. The titration is monitored by a glass electrode and the total alkalinity of the sample is calculated using a non-linear least-squares method following Dickson et al. (2007). Open-cell potentiometric acid titrator
Calcium [Ca2+] concentration measurements were made with a Metrohm Titrando calcium-selective electrode on a titration system (Metrohm Titrando 888) and measured using automatic potentiometric titration with ethylene glycol tetra acetic acid (EGTA), with a precision of +/-0.2% Titration System (Metrohm Titrando 888)
Calcium [Ca2+] concentration was measured with a Metrohm Titrando calcium-selective electrode on a titration system using automatic potentiometric titration with ethylene glycol tetra acetic acid (EGTA), with a precision of +/-0.2%. Metrohm Titrando 888
Behavior Metrics - Crab-Oyster behavior - from UNC Reis laboratory starting 2010 (OA - Ocean Acidification and Warming Impact on Calcification project)The temperature within experimental tanks was measured every other day with a NIST-calibrated partial-immersion organic- filled glass thermometer (precision + 0.3%, accuracy + 0.4%). Salinity was measured every other day with a YSI 3200 conduc- tivity meter with a YSI 3440 cell (K 1⁄4 10) that was calibrated with seawater standards of known salinity provided by the labora- tory of Prof. A. Dickson of Scripps Institute of Oceanography. Seawater pH was measured every other day with a Thermo Scien- tific Orion 2 Star benchtop pH meter with an Orion 9156BNWP pH probe, calibrated with 7.00 and 10.01 Orion NBS buffers traceable to NIST standard reference material (for slope of the calibration curve) and with seawater standards of known pH also provided by Prof. Dickson’s laboratory (for y-intercept of the calibration curve). Seawater DIC was measured via coulometry (UIC 5400) and TA was measured via closed-cell potentiometric Gran titration calibrated with certified Dickson TA/DIC standards. Measure- ment of DIC and TA of the certified reference materials (CRMs) were consistently within 0.3% of certified values. Differences between the measured and certified TA and DIC values of the CRMs were used to correct measurements of experimental seawater solutions. Closed-cell potentiometric Gran titration
Consumption data from Crab-Oyster Behavior study from laboratory results from UNC from the Sapodilla Caye, Belize ongoing from 2010 (OA - Ocean Acidification and Warming Impact on Calcification project)The temperature within experimental tanks was measured every other day with a NIST-calibrated partial-immersion organic- filled glass thermometer (precision + 0.3%, accuracy + 0.4%). Salinity was measured every other day with a YSI 3200 conduc- tivity meter with a YSI 3440 cell (K 1⁄4 10) that was calibrated with seawater standards of known salinity provided by the labora- tory of Prof. A. Dickson of Scripps Institute of Oceanography. Seawater pH was measured every other day with a Thermo Scien- tific Orion 2 Star benchtop pH meter with an Orion 9156BNWP pH probe, calibrated with 7.00 and 10.01 Orion NBS buffers traceable to NIST standard reference material (for slope of the calibration curve) and with seawater standards of known pH also provided by Prof. Dickson’s laboratory (for y-intercept of the calibration curve). Seawater DIC was measured via coulometry (UIC 5400) and TA was measured via closed-cell potentiometric Gran titration calibrated with certified Dickson TA/DIC standards. Measure- ment of DIC and TA of the certified reference materials (CRMs) were consistently within 0.3% of certified values. Differences between the measured and certified TA and DIC values of the CRMs were used to correct measurements of experimental seawater solutions. Closed-cell potentiometric Gran titration
Seawater chemistry calculated parameters from Crab-Oyster Behavior study from laboratory results from UNC from data collected at the Sapodilla Caye, Belize starting 2010 (OA - Ocean Acidification and Warming Impact on Calcification project)The temperature within experimental tanks was measured every other day with a NIST-calibrated partial-immersion organic- filled glass thermometer (precision + 0.3%, accuracy + 0.4%). Salinity was measured every other day with a YSI 3200 conduc- tivity meter with a YSI 3440 cell (K 1⁄4 10) that was calibrated with seawater standards of known salinity provided by the labora- tory of Prof. A. Dickson of Scripps Institute of Oceanography. Seawater pH was measured every other day with a Thermo Scien- tific Orion 2 Star benchtop pH meter with an Orion 9156BNWP pH probe, calibrated with 7.00 and 10.01 Orion NBS buffers traceable to NIST standard reference material (for slope of the calibration curve) and with seawater standards of known pH also provided by Prof. Dickson’s laboratory (for y-intercept of the calibration curve). Seawater DIC was measured via coulometry (UIC 5400) and TA was measured via closed-cell potentiometric Gran titration calibrated with certified Dickson TA/DIC standards. Measure- ment of DIC and TA of the certified reference materials (CRMs) were consistently within 0.3% of certified values. Differences between the measured and certified TA and DIC values of the CRMs were used to correct measurements of experimental seawater solutions. Closed-cell potentiometric Gran titration
Seawater chemistry measured parameters from Crab-Oyster Behavior study from laboratory results from UNC from data collected at the Sapodilla Caye, Belize starting 2010 ((OA - Ocean Acidification and Warming Impact on Calcification project)The temperature within experimental tanks was measured every other day with a NIST-calibrated partial-immersion organic- filled glass thermometer (precision + 0.3%, accuracy + 0.4%). Salinity was measured every other day with a YSI 3200 conduc- tivity meter with a YSI 3440 cell (K 1⁄4 10) that was calibrated with seawater standards of known salinity provided by the labora- tory of Prof. A. Dickson of Scripps Institute of Oceanography. Seawater pH was measured every other day with a Thermo Scien- tific Orion 2 Star benchtop pH meter with an Orion 9156BNWP pH probe, calibrated with 7.00 and 10.01 Orion NBS buffers traceable to NIST standard reference material (for slope of the calibration curve) and with seawater standards of known pH also provided by Prof. Dickson’s laboratory (for y-intercept of the calibration curve). Seawater DIC was measured via coulometry (UIC 5400) and TA was measured via closed-cell potentiometric Gran titration calibrated with certified Dickson TA/DIC standards. Measure- ment of DIC and TA of the certified reference materials (CRMs) were consistently within 0.3% of certified values. Differences between the measured and certified TA and DIC values of the CRMs were used to correct measurements of experimental seawater solutions. Closed-cell potentiometric Gran titration
Unsuccessful events from Crab-Oyster Behavior study from UNC laboratory results from data from the Sapodilla Caye, Belize starting 2010 (OA - Ocean Acidification and Warming Impact on Calcification project)The temperature within experimental tanks was measured every other day with a NIST-calibrated partial-immersion organic- filled glass thermometer (precision + 0.3%, accuracy + 0.4%). Salinity was measured every other day with a YSI 3200 conduc- tivity meter with a YSI 3440 cell (K 1⁄4 10) that was calibrated with seawater standards of known salinity provided by the labora- tory of Prof. A. Dickson of Scripps Institute of Oceanography. Seawater pH was measured every other day with a Thermo Scien- tific Orion 2 Star benchtop pH meter with an Orion 9156BNWP pH probe, calibrated with 7.00 and 10.01 Orion NBS buffers traceable to NIST standard reference material (for slope of the calibration curve) and with seawater standards of known pH also provided by Prof. Dickson’s laboratory (for y-intercept of the calibration curve). Seawater DIC was measured via coulometry (UIC 5400) and TA was measured via closed-cell potentiometric Gran titration calibrated with certified Dickson TA/DIC standards. Measure- ment of DIC and TA of the certified reference materials (CRMs) were consistently within 0.3% of certified values. Differences between the measured and certified TA and DIC values of the CRMs were used to correct measurements of experimental seawater solutions. Closed-cell potentiometric Gran titration
Monthly cross-shore transects of biogeochemical properties in La Jolla, CAOver a 5 minute time period, the AFT-pH uses a pump and extracts 10 mL of the seawater sample, introduces the sample with mCP indicator dye and is spectrophotometrically analyzed within a pH range of 7.4 to 8.5. AFT-pH (SAMI, Sunburst)
Monthly cross-shore transects of biogeochemical properties in La Jolla, CAThe open-cell potentiometric acid titration system was developed by the laboratory of A.G. Dickson. Briefly, a known amount of seawater is added to an open cell temperature controlled beaker. Hydrochloric acid (0.1 N) is added using a Methrom Dosimat to a pH of 3.5-4.0. Following equilibration, small increments of hydrochloric acid are then added to the seawater until pH equals ~ 3.0. The titration is monitored by a glass electrode and the total alkalinity of the sample is calculated using a non-linear least-squares method following Dickson et al. (2007). Open-cell potentiometric acid titrator
Larval positioning in acrylic tubes describing the behavior of coral larvae in high pCO2 within shallow tropical reefs in Okinawa, Japan from 2016-07 to 2016-08The salinity of the seawater used to fill the larval incubation tubes was measured using a conductivity meter (TetraCon 325, WTW, Germany), and AT was determined using open-cell titrations conducted with an autoburette titrator (Kimoto, ATT-05, Japan). autoburette titrator
Chemical analysis of sediment core pore water from the Shinnecock Bay tidal flat in February of 2019Radiometer CMT-10 titrator
Lipid content describing the behavior of coral larvae in high pCO2 within shallow tropical reefs in Okinawa, Japan from 2016-07 to 2016-08The salinity of the seawater used to fill the larval incubation tubes was measured using a conductivity meter (TetraCon 325, WTW, Germany), and AT was determined using open-cell titrations conducted with an autoburette titrator (Kimoto, ATT-05, Japan). autoburette titrator
Laboratory study of long-term growth in juvenile Menidia menidia (Atlantic silverside) at contrasting CO2 levels for 16 to 122 days in 2015Potentiometric Titrator Mettler Toledo G20
Measured and calculated geochemistry values and uncertainty for water samples taken from the water column of Celestun Lagoon, Yucatan, Mexico in May of 2015Orion 950 Titrator
Macrophytes' amelioration of seawater acidity: Comparison among four species of marine macrophytes (Ulva lactuca, Zostera marina, Fucus vesiculosus and Saccharina latissima) from the Gulf of Maine from June to July 2015Basic model for setting up customer-specific automation in the titration. Metrohm 855 Robotic Titrosampler, Metrohm, USA
Calcification Rates and Biomass of 4 Coral Species, 2 Temperatures and 2 pCO2 Levels from Experiments at LTER site in Moorea, French Polynesia, 2011 (OA_Corals project)Open cell potentiometric titrator (Model T50, Mettler-Toledo, Columbus, OH) fitted with a DG115-SC pH probe (Mettler-Toledo, Columbus, OH)
Tank seawater conditions from Coral/Temperature/pCO2 Experiments at LTER site in Moorea, French Polynesia, 2011 (OA_Corals project)Open cell potentiometric titrator (Model T50, Mettler-Toledo, Columbus, OH) fitted with a DG115-SC pH probe (Mettler-Toledo, Columbus, OH)
Biogeochemistry data from Maunalua Bay, Hawaii measured on four dates between April and October 2015Total Alkalinity was measured on a Mettler Toledo T-50 autotitrator and calibrated against a certified reference material from Andrew Dickson’s Lab. Sample accuracy was 0.48%.  Mettler Toledo T-50 autotitrator
Chemical and physical measurements in coral tanks from Moorea, French Polynesia in 2010 (OA Corals project)In Moorea, an automatic titrator (Model T50, Mettler-Toledo) was used.,br /> In Okinawa, an AT titration analyzer (Kimoto ATT-05).
Water chemistry during mesocosm study of trophic interactions under ocean acidification in Bodega Bay, CA.Metrohm 855 autotitrator
Morphometric data from experiments on CO2 sensitivity of Northern sand lance (Ammodytes dubius) embryos conducted in 2018 and 2020Mettler Toledo G20 Potentiometric Titrator
Experimental results on the carbonate chemistry of mussel growth from the Bodega Marine Laboratory in 2013 (OMEGAS-MaS project)automated Gran titration (Metrohm 809) Automatic titrator
Benthic chamber geochemical data obtained with an in situ benthic lander from the R/V Savannah at sampling stations across the Louisiana shelf and slope in the Northern Gulf of Mexico during four cruise between July 2021 and July 2022DIC and TA measurements were obtained with a computer-controlled Metrohm 877 Titrino Plus. Metrohm 877 Titrino Plus
Particulate phosphate, particulate carbon, particulate nitrogen, particulate bulk isotope composition (C and N), and dissolved oxygen from Niskin bottle samples collected on R/V Kilo Moana cruise KM1910 in June 20195 ml Dosimat titrator (Brinkmann) Dosimat titrator (Brinkmann)
Depth profiles of pore water constituents from sediment cores collected on the Louisiana Shelf of the Northern Gulf of Mexico during November 2020 on R/V Savannah cruise SAV-20-07TA measurements were obtained with a computer-controlled Metrohm 877 Titrino plus. Metrohm 877 Titrino plus
Data on how nutrient and sediment loading affect coral functionality in a tropical branching coralAn automatic titrator (Mettler-Toledo T50,Columbus, OH, USA) fitted with a InMotion Pro-sample carousel (Columbus, OH, USA). The titrator had a Mettler pH probe (DGi-115, Columbus, OH, USA) and was operated with certified HCl titrant (Batch #A17, Dickson Laboratory).  Mettler-Toledo T50
Phenotypic responses of Eastern oyster in response to variable length OA exposure conducted in summer 2017 with oysters sampled in Plum Island. VINDTA 3C coupled alkalinity gram titration and coulometric DIC analyzer system
Water quality of experiment measuring phenotypic responses of Eastern oyster in response to variable length OA exposure conducted in summer 2017 with oysters sampled in Plum Island. VINDTA 3C coupled alkalinity gram titration and coulometric DIC analyzer system
OOI Global Argentine Basin Array CTD and Discrete Water Sampling Data from Mooring Overturning Cruises in the Argentine Basin from 2015-2018 (OOI Cruise Data project)Dissolved oxygen measurements are performed following the methodology outlined in the WHOI Hydrography Blue Book _Automated Oxygen Titration and Salinity Determination_ (Knapp et al. 1990). Measurements are performed using a Metrohm Model 888 Titrando dosing device, with the titration endpoint determined amperometrically. Stated accuracy is 0.02 ml/l, with a precision of 0.001 ml/l. Metrohm Model 888 Titrando dosing device
OOI Global Irminger Sea Array CTD and Discrete Water Sampling Data from Mooring Overturning Cruises in the Irminger Sea from 2014-2023 (OOI Cruise Data project)Dissolved oxygen measurements are performed following the methodology outlined in the WHOI Hydrography Blue Book _Automated Oxygen Titration and Salinity Determination_ (Knapp et al. 1990). Measurements are performed using a Metrohm Model 888 Titrando dosing device, with the titration endpoint determined amperometrically. Stated accuracy is 0.02 ml/l, with a precision of 0.001 ml/l. Metrohm Model 888 Titrando dosing device
OOI Coastal Pioneer NES Array CTD and Discrete Water Sampling Data from Mooring Overturning Cruises Dissolved oxygen measurements are performed following the methodology outlined in the WHOI Hydrography Blue Book _Automated Oxygen Titration and Salinity Determination_ (Knapp et al. 1990). Measurements are performed using a Metrohm Model 888 Titrando dosing device, with the titration endpoint determined amperometrically. Stated accuracy is 0.02 ml/l, with a precision of 0.001 ml/l. Metrohm Model 888 Titrando dosing device
OOI Global Southern Ocean Array CTD and Discrete Water Sampling Data from Mooring Overturning Cruises in the Southern Pacific Ocean from 2015-2020 (OOI Cruise Data project)Dissolved oxygen measurements are performed following the methodology outlined in the WHOI Hydrography Blue Book Automated Oxygen Titration and Salinity Determination (Knapp et al. 1990). Measurements are performed using a Metrohm Model 888 Titrando dosing device, with the titration endpoint determined amperometrically. Stated accuracy is 0.02 ml/L, with a precision of 0.001 ml/L. Metrohm Model 888 Titrando dosing device
OOI Global Station Papa Array CTD and Discrete Water Sampling Data from Mooring Overturning Cruises in the Gulf of Alaska from 2013 to 2023 (OOI Cruise Data project)Dissolved oxygen measurements are performed following the methodology outlined in the WHOI Hydrography Blue Book _Automated Oxygen Titration and Salinity Determination_ (Knapp et al. 1990). Measurements are performed using a Metrohm Model 888 Titrando dosing device, with the titration endpoint determined amperometrically. Stated accuracy is 0.02 ml/l, with a precision of 0.001 ml/l. Metrohm Model 888 Titrando dosing device
Open cell and single-step method for total alkalinity titrations from samples collected on R/V Mirai cruises MR23-06C and MR23-07 in the North Pacific and Western Arctic from September to November 2023The shipboard TA analysis by single step titration was done using spectrophotometry with a single acid addition procedure using a custom-made system (Nippon ANS, Inc., Japan). Land-based TA was performed with a custom-designed titration designed and built by the laboratory of Andrew G. Dickson (University of California, San Diego) as described in Dickson et al. (2003). custom-designed titration
Results of a study examining how foundation species loss alters multiple ecosystem functions based on community surveys and biogeochemical sampling of tidepools in the Otter Rock Marine Reserve (ORMR) and Marine Garden, Oregon USA from June to August 2019Mettler-Toledo T5 auto-titrator
Other solutes from vertical profiles from multi- and gravity cores from two cruises, R/V Robert Gordon Sproul SP1215 and R/V New Horizon NH1319, in the Santa Barbara and Santa Monica Basins in 2012 and 2013Automated Gran Titration
Juvenile growth post-settlement from experimental study of carryover effects of ocean acidification on larval and juvenile Olympia oysters; conducted at Bodega Marine Lab in July-Sept 2009Alkalinity was measured using automated Gran titration (Metrohm 809; Metrohm, Herisau, Switzerland) Metrohm 809
Juvenile growth post-settlement under four larval-to-juvenile ocean acidification transitions in the laboratory; conducted at Bodega Marine Lab in July-Sept 2009Alkalinity was measured using automated Gran titration (Metrohm 809; Metrohm, Herisau, Switzerland) Metrohm 809
Oyster larval growth at day 9 post-release under ocean acidification in the laboratory; experiments conducted at Bodega Marine LaboratoryAlkalinity was measured using automated Gran titration (Metrohm 809; Metrohm, Herisau, Switzerland) Metrohm 809
Water chemistry during larval rearing experiments concerning persistent effects of ocean acidification on larval and juvenile Olympia oysters conducted at Bodega Marine Laboratory in September 2010Total alkalinity (TA, umol per kg of seawater) was measured using automated Gran titration with duplicates (Metrohm 809) Metrohm 809
Effects of food on growth of larval Olympia oysters under different levels of ocean acidification; experiments conducted at Bodega Marine Laboratory (BML), UC Davis in June 2011TA was measured using automated Gran titration with duplicates (Metrohm 809) Metrohm 809
Effects of food on body condition of larval oysters under different levels of ocean acidification; experiments conducted at Bodega Marine Laboratory (BML), UC Davis in June 2011TA was measured using automated Gran titration with duplicates (Metrohm 809) Metrohm 809
Effects of food on percent metamorphosis of settling Olympia oysters under different levels of ocean acidification; experiments conducted at Bodega Marine Laboratory, UC Davis in June 2011TA was measured using automated Gran titration with duplicates (Metrohm 809) Metrohm 809
Calcium carbonate dissolution experiment Phase 1: Effect of elevated pCO2 on sediment dissolution.Mettler Toledo T50
Calcium carbonate dissolution experiment Phase 2: Effect of elevated pCO2 and NO3- on sediment dissolution.Mettler Toledo T50
Pleurochrysis carterae diel culture dynamics analyzed at Bigelow Laboratory from 2013 (OA Copes Coccoliths project)Automatic titrations controlled by Tiamo software (Metrohm, Riverview, FL, USA). Metrohm Titrando 888
Pleurochrysis carterae growth cycle culture dynamics analyzed at Bigelow Laboratory from 2013 (OA Copes Coccoliths project)Automatic titrations controlled by Tiamo software (Metrohm, Riverview, FL, USA). Metrohm Titrando 888
Carbonate chemistry in experimental cultures of Plocamium cartilagineum cultured at different temperatures and pCO2 levels (Seaweed OA Resilience project)Used to measure total alkalinity; equipped with Rondolino automated titration stand. Mettler Toledo T50
Carbonate chemistry over a time-course in pH drift experiments with Plocamium growth collected at Catalina Island, 2014-2015 (Seaweed OA Resilience project)Used to measure total alkalinity; equipped with Rondolino automated titration stand. Mettler Toledo T50
Porewater H2S from a multi-core sample taken on R/V Hugh Sharp cruise HRS1709 in the Chesapeake Bay in August 2017AS-ALK2 Apollo Scitech
Seasonal hydrography, abundance, and distribution of pteropods from MOCNESS and CTD casts during R/V Tioga cruises in the Gulf of Maine from 2013 to 2015Apollo SciTech alkalinity auto-titrator
O2 consumption of pteropods held for 1-14 days in three CO2 treatments with pteropods collected with a Reeve net during R/V Tioga cruises in the Gulf of Maine from 2013 to 2014Apollo SciTech alkalinity auto-titrator
Data from in situ pump profiler system collected on R/V Hugh R. Sharp cruise HRS1415 in August 2014AS-ALK2 Apollo Scitech titrator
Reconstructed genomes from North Pond, western flank of the Mid-Atlantic Ridge, from 2012-2014Fluids also were analyzed for dissolved silicon and nitrate using automated colorimetric analysis and pH was measured with an electrode before a potentiometric titration for the determination of alkalinity (Wheat et al., 2017). automated colorimetric analysis
Reef organism growth from in-situ experiments with submarine groundwater discharge (SGD) exposure treatments conducted in Mo'orea, French Polynesia in 2022 and 2023Mettler Toledo T5 autotitrator:  (accuracy < 0.5% off from  certified reference material, precision = 5 µmol kg-1) was used to measure total alkalinity. Mettler Toledo T5 autotitrator
Biomass-normalized dark respiration and net photosynthesis rates in coral and algae as a function of pCO2 tested in both 2011 and 2012, Moorea LTER siteopen cell, potentiometric titrations (automatic titrator T50, Mettler-Toledo)
Biomass-normalized dark respiration and net photosynthesis rates in coral and algae as a function of pCO2 where LEDR (light-enhanced dark respiration) was measured (OA coral adaptation project)open cell, potentiometric titrations (automatic titrator T50, Mettler-Toledo)
CTD rosette and Bottle data from R/V Hugh R. Sharp cruise HRS1314 in the Chesapeake Bay and coastal Atlantic Ocean in August 2013TA - Open cell Gran titration with semi-automatic AS-ALK2 Apollo Scitech titrator AS-ALK2 Apollo Scitech titrator
CTD data and analyses of bottles from CTD rosette samples collected on R/V Hugh R. Sharp cruise HRS1415 in August 2014AS-ALK2 Apollo Scitech titrator
CTD-associated variables, bottle salinity measurements, oxygen titrations, nutrient analyses, biogeochemical/biological variables, and DIC/Freon chemistry variables from R/V Roger Revelle cruise RR2004 along the 150W meridian from 30S to 60SVINDTA 3S
Discrete bottle sample measurements for carbonate chemistry, organic alkalinity and organic carbon from samples collected in Waquoit Bay and Vineyard Sound, MA in 2016
Discrete bottle sample measurements for carbonate chemistry from samples collected in the Sage Lot Pond salt marsh tidal creek in Waquoit Bay, MA from 2012 to 2015Apollo SciTech alkalinity auto-titrator (Model AS-ALK2)
Carbon export from San Dieguito Lagoon from samples for seawater carbonate biogeochemistry between April 2014 and January 2015The open-cell potentiometric acid titration system was developed by the laboratory of A.G. Dickson. Briefly, a known amount of seawater is added to an open cell temperature controlled beaker. Hydrochloric acid is added using a Methrom Dosimat to a pH of 3.5-4.0 and allowed to stabilize to remove CO2 gas formed by the addition of acid. Small aliquots of hydrochloric acid are then added to pH of ~ 3.0. The titration is monitored by a glass electrode and the total alkalinity of the sample is calculated using a non-linear least-squares method following Dickson et al. (2007). Open-cell potentiometric acid titration system
The effect of altered carbonate system chemistry on the abiotic formation of Transparent Exopolymer Particles (TEP) from UCSB MSI Passow Laboratory from 2009 to 2010 (OA - Ocean Acidification and Aggregation project) In brief, TA (μmol kg− 1) was measured within 4 hours after sampling by potentiometric titration (T50, Mettler Toledo) using certified acid titrant. Automatic titrator - T50, Mettler Toledo
The effect of altered carbonate system chemistry on the abiotic formation of Transparent Exopolymer Particles (TEP).from UCSB MSI Passow Lab from 2009 to 2010 (OA - Ocean Acidification and Aggregation project)In brief, TA (μmol kg− 1) was measured within 4 hours after sampling by potentiometric titration (T50, Mettler Toledo) using certified acid titrant. Automatic titrator - T50, Mettler Toledo
Aggregate Sinking Velocity from the CO2 Aggregation Experiment from UCSB Marine Science Institute Passow Lab from 2009 to 2010 (OA - Ocean Acidification and Aggregation project)TA was calculated from linear Gran plots (Gran 1952) after duplicate potentiometric titration (Brewer et al. 1986) using a TitroLine alpha plus (Schott Instruments, Mainz, Germany). Average precision was ± 5 μmol kg-1. Certified Reference Materials (CRMs, Batch No. 54) supplied by A. Dickson (Scripps Institution of Oceanography, USA) was used as a control. Automatic titrator - TitroLine alpha plus (Schott Instruments, Mainz, Germany)
Aggregate Size from the CO2 Aggregation Experiment from UCSB Marine Science Institute Passow Lab from 2009 to 2010 (OA - Ocean Acidification and Aggregation project)TA was calculated from linear Gran plots (Gran 1952) after duplicate potentiometric titration (Brewer et al. 1986) using a TitroLine alpha plus (Schott Instruments, Mainz, Germany). Average precision was ± 5 μmol kg-1. Certified Reference Materials (CRMs, Batch No. 54) supplied by A. Dickson (Scripps Institution of Oceanography, USA) was used as a control. Automatic titrator - TitroLine alpha plus (Schott Instruments, Mainz, Germany)
BioChemical results from the CO2 Aggregation Experiment from UCSB Marine Science Institute Passow Lab from 2009 to 2010 (OA - Ocean Acidification and Aggregation project)TA was calculated from linear Gran plots (Gran 1952) after duplicate potentiometric titration (Brewer et al. 1986) using a TitroLine alpha plus (Schott Instruments, Mainz, Germany). Average precision was ± 5 μmol kg-1. Certified Reference Materials (CRMs, Batch No. 54) supplied by A. Dickson (Scripps Institution of Oceanography, USA) was used as a control. Automatic titrator - TitroLine alpha plus (Schott Instruments, Mainz, Germany)
Carbonate System results from the CO2 Aggregation Experiment from UCSB Marine Science Institute Passow Lab from 2009 to 2010 (OA - Ocean Acidification and Aggregation project)TA was calculated from linear Gran plots (Gran 1952) after duplicate potentiometric titration (Brewer et al. 1986) using a TitroLine alpha plus (Schott Instruments, Mainz, Germany). Average precision was ± 5 μmol kg-1. Certified Reference Materials (CRMs, Batch No. 54) supplied by A. Dickson (Scripps Institution of Oceanography, USA) was used as a control. Automatic titrator - TitroLine alpha plus (Schott Instruments, Mainz, Germany)
General Information about the CO2 Aggregation Experiment from UCSB Marine Science Institute Passow Lab from 2009 to 2010 (OA - Ocean Acidification and Aggregation project)TA was calculated from linear Gran plots (Gran 1952) after duplicate potentiometric titration (Brewer et al. 1986) using a TitroLine alpha plus (Schott Instruments, Mainz, Germany). Average precision was ± 5 μmol kg-1. Certified Reference Materials (CRMs, Batch No. 54) supplied by A. Dickson (Scripps Institution of Oceanography, USA) was used as a control. Automatic titrator - TitroLine alpha plus (Schott Instruments, Mainz, Germany)
pH data from the CO2 Aggregation Experiment from UCSB Marine Science Institute Passow Lab from 2009 to 2010 (OA - Ocean Acidification and Aggregation project)TA was calculated from linear Gran plots (Gran 1952) after duplicate potentiometric titration (Brewer et al. 1986) using a TitroLine alpha plus (Schott Instruments, Mainz, Germany). Average precision was ± 5 μmol kg-1. Certified Reference Materials (CRMs, Batch No. 54) supplied by A. Dickson (Scripps Institution of Oceanography, USA) was used as a control. Automatic titrator - TitroLine alpha plus (Schott Instruments, Mainz, Germany)
Effects of ocean acidification on anti-predator behavior of snails from Bodega Bay, CA.Total alkalinity was measured using a Metrohm 855 autotitrator Metrohm 855 autotitrator
Spatial surveys of carbonate chemistry conducted in Kaneohe Bay, Hawaii from small boats during 2015 to 2017The open-cell potentiometric acid titration system was developed by the laboratory of A.G. Dickson. Briefly, a known amount of seawater is added to an open cell temperature controlled beaker. Hydrochloric acid is added using a Methrom Dosimat to a pH of 3.5-4.0 and allowed to stabilize to remove CO2 gas formed by the addition of acid. Small aliquots of hydrochloric acid are then added to pH of ~3.0. The titration is monitored by a glass electrode and the total alkalinity of the sample is calculated using a non-linear least-squares method following Dickson et al. (2007). Open-cell potentiometric acid titration system
Data from the spawning trial in a study of CO2 and temperature-specific reproductive traits in Menidia menidiaG20 Potentiometric Titrator (Mettler Toledo)
Data on egg production resulting from the spawning trial in a study of CO2 and temperature-specific reproductive traits in Menidia menidiaG20 Potentiometric Titrator (Mettler Toledo)
Partial pressure of carbon dioxide (pCO2) from in-situ flume experiments to manipulate pCO2 on shallow tropical coral reef communities at UCB Gump Research Station Moorea, French Polynesia in May of 2018TA generated using a Mettler Toledo T50 fitted with a DG 115-SC electrode. Mettler Toledo T50
Alkalinity and salinity from in-situ flume experiments to manipulate pCO2 on shallow tropical coral reef communities at UCB Gump Research Station Moorea, French Polynesia in May of 2018TA generated using a Mettler Toledo T50 fitted with a DG 115-SC electrode. Mettler Toledo T50
Survival data from static and fluctuating pCO2 x dissolved oxygen (DO) experiments on Menidia menidiaAlkalinity – Metler Toledo G20 Potentiometric Titrator calibrated with certified reference material from Dr. Andrew Dickson, University of California San Diego Metler Toledo G20 Potentiometric Titrator
Survival, length, and growth responses of M. menidia offspring from different females exposed to contrasting CO2 environments.Used to measure total alkalinity Mettler Toledo™ G20 Potentiometric Titrator
Data on hatching frequency and success from an experiment on CO2 sensitivity of Northern sand lance (Ammodytes dubius) embryos conducted in 2020Mettler Toledo G20 Potentiometric Titrator
Symbiodinium cell density, chlorophyll, respiration, photosynthetic yield in Seriatopora coral, Taiwan, 2010 (MCR LTER project, Climate_Coral_Larvae project)Open-cell autotitrator (Model DL50, Mettler-Toledo, USA) filled with certified acid titrant (from A. Dickson, Scripps Institution of Oceanography) and equipped with a DG115-SC pH probe (Mettler-Toledo). Automatic titrator
Seriatopora bleaching expt: Photosynthesis:irradiance (P/I) curves, Taiwan 2010 (MCR LTER project, Climate_Coral_Larvae project)Open-cell autotitrator (Model DL50, Mettler-Toledo, USA) filled with certified acid titrant (from A. Dickson, Scripps Institution of Oceanography) and equipped with a DG115-SC pH probe (Mettler-Toledo). Automatic titrator
Seriatopora bleaching expt: temperature, salinity, light in tanks, Taiwan 2010 (MCR LTER project, Climate_Coral_Larvae project)Open-cell autotitrator (Model DL50, Mettler-Toledo, USA) filled with certified acid titrant (from A. Dickson, Scripps Institution of Oceanography) and equipped with a DG115-SC pH probe (Mettler-Toledo). Automatic titrator
Seriatopora bleaching expt: water pH and carbonate chemistry, Taiwan 2010 (MCR LTER project, Climate_Coral_Larvae project)Open-cell autotitrator (Model DL50, Mettler-Toledo, USA) filled with certified acid titrant (from A. Dickson, Scripps Institution of Oceanography) and equipped with a DG115-SC pH probe (Mettler-Toledo). Automatic titrator
Data on laboratory cultures and statistical analysis code associated with the paper "Co-culture with Synechococcus facilitates the growth of Prochlorococcus under ocean acidification conditions" published in Environmental MicrobiologyAlkalinity was measured using a Mettler-Toledo titrator. Mettler-Toledo titrator
Synthesis Product for Ocean Time Series (SPOTS) Metrohm OMNIS Titrator used by HOT & RADCOR for Oxygen measurements Tritrino Winkler used by DYFAMED for Oxygen measurements Metrohm Titrando Dual Titrator used by HOT for Oxygen measurements Dosimat 665 used by HOT for Oxygen measurements VINDTA (3S) used by CVOO and OWSM for Total Alkalinity measurements Metrohm 794 used by GIFT for Total Alkalinity measurements Titration instruments
Total alkalinity from an experiment testing the suitability of high-density polyethylene (HDPE) for collection and long-term storage of total alkalinity samples using laboratory-manipulated oligotrophic Atlantic surface waterSamples were analyzed in the lab for total alkalinity using a custom designed open cell titration with non-linear least squares fitting designed and built by the laboratory of Andrew G. Dickson (University of California, San Diego) and described in detail in Dickson et al. (2003). custom designed open cell titration
Total alkalinity determined through experiments comparing usage of high-density polyethylene (HDPE) and borosilicate glass bottles for collection and storage of water samples collected on the GEOTRACES GP17-OCE cruise (R/V Roger Revelle RR2214)Samples were analyzed in the lab for total alkalinity using a custom-designed open cell titration with non-linear least squares fitting designed and built by the laboratory of Andrew G. Dickson (University of California, San Diego) and described in detail in Dickson et al. (2003). custom-designed open cell titration
Carbonate chemistry of Ulva lactuca culture pots testing the effects of pCO2 variability (Seaweed OA Resilience project)Used to measure total alkalinity. Mettler Toledo T50 equipped with Rondolino
Carbonate chemistry over a time course with Ulva in pH drift experiments (Seaweed OA Resilience project)Used to measure total alkalinity. Mettler Toledo T50 equipped with Rondolino
Data on unhatched embryos from an experiment on CO2 sensitivity of Northern sand lance (Ammodytes dubius) embryos conducted in 2018Mettler Toledo G20 Potentiometric Titrator
Water column data from samples collected on R/V Hugh Sharp cruise HRS1709 in the Chesapeake Bay in August 2017AS-ALK2 Apollo Scitech
Water column data from samples collected on R/V Hugh Sharp cruise HRS1803GL in the Chesapeake Bay during July-August 2018AS-ALK2 Apollo Scitech titrator
Weekly coral calcification rates in the CO2 manipulation experiment conducted on Porites corals from PalauAutomated gran titrations for TA were run on duplicate 1 ml samples using a Metrohm Titrando 808 and 730 Sample Changer. Metrohm Titrando 808 and 730 Sample Changer